Standardization of an Effective Protocol for in vitro Culture of  Lilium longiflorum Thunb cv. Pavia

Bhandari, Narendra Singh; Aswath, C.

doi:10.20546/ijcmas.2018.704.131

Cited by 4 publications

(4 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Tender explants show more phytotoxic effects of sterilants compared to mature ones and hence less survival. Bhandari and Aswath (2018) used three different forms of explants to create an efficient in-vitro culture protocol for Lilium longiflorum Cv. ‘Pavia’, including leaves, outer and inner bulb scales.…”

Section: Discussionmentioning

confidence: 99%

“…Lilium is cultivated extensively in a wide range of climates. In the world floriculture trade, it is ranked fourth among the top ten cut flowers ( Bhandari et al, 2018 ). Lilium is regarded as a popular floral trade cut flower, and several firms of breeding are working to develop new varieties of species from various divisions of the genus.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Standardization of in vitro micropropagation procedure of Oriental Lilium Hybrid Cv. ‘Ravenna’

Rafiq

Rather

Bhat

et al. 2021

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

Micropropagation protocol of Oriental Hybrid Lilium cv. Ravenna was developed using bulb scale segments (Basal and Tip) as explants. Surface sterilization of healthy bulb scales with carbendazim 200 ppm for 30 min, then 0.1 percent mercuric chloride for 10 min, then 70% ethyl alcohol for 30 s was superior to all other treatments in recording highest culture asepsis (77.08%) and higher explant survival (86.12%). Explant survival was higher in basal segments (88.54%) compared to tip segments (85.52%). Highest culture establishment was recorded in basal scale segments (68.26%) followed by tip scale segments (55.21%). MS medium augmented with 0.50 mgl −1 Naphthalene acetic acid and 2.0 mgl −1 . 6-Benzylamino Purine recorded maximum culture establishment (76.17%), highest bulblet number/explant (5.52) with maximum length of shoots (2.20 cm) and number of leaves (3.39). This treatment combination of growth regulators resulted in highest shoot proliferation (83.33%) along with maximum shoot number (2.41explant −1 ), shoot length (2.35 cm) and leaf number (5.44) of micro shoots during proliferation stage. Rooting of explants was superior with Indole-3-butyric acid compared to Naphthalene acetic acid. Highest rooting of 92.71% along with maximum number of primary roots shoot −1 (12.06), maximum primary root length (3.17 cm) was documented in Murashige and Skoog medium added with Indole-3-butyric acid 1.50 mgl −1 with best ex vitro survival rate (98.96%) of rooted plantlets during primary hardening in perlite + vermiculite (1:1) mixture.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Standardization of in vitro micropropagation procedure of Oriental Lilium Hybrid Cv. ‘Ravenna’

Rafiq

Rather

Bhat

et al. 2021

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

show abstract

“…Pendekatan melalui teknik kultur jaringan secara in vitro merupakan upaya perbanyakan tanaman yang potensial untuk mendukung pengadaan benih L. longiflorum. Teknik ini dapat memproduksi tanaman dengan kualitas yang baik (Kanchanapoom et al 2011), menjanjikan bibit secara massal, cepat dan memiliki keseragaman genetik yang bebas dari kontaminan (Minarsih et al 2016), serta terbukti paling efisien untuk propagasi dan multiplikasi pada genus Lilium (Bhandari & Aswath 2018).…”

Section: Pendahuluanunclassified

Induksi Kalus Sisik Umbi Lilium longiflorum Thunb. oleh Auksin dan Sitokinin, serta Respons Pertumbuhannya Secara In Vitro

Ekawati¹,

Anggraeni

Prawestri

et al. 2022

agrosainstek

View full text Add to dashboard Cite

Lilium longiforum Thunb. adalah florikultura potensial untuk dikembangkan di bidang industri farmasi dan florikultura. Perbanyakan L. longiflorum secara generatif sulit dilakukan dan perbanyakan vegetatif dengan kultur jaringan jauh lebih efektif. Oleh karena itu, diperlukan sebuah protokol perbanyakan L. longiflorum secara in vitro yang efisien. Tujuan penelitian ini adalah mengamati induksi kalus dari eksplan sisik umbi dari planlet L. longiflorum dan respons pertumbuhannya terhadap penambahan auksin dan sitokinin dalam media kultur. Respons sisik umbi pada induksi kalus diuji dengan dua perlakuan, yaitu MS + 3,0 mg L-1 2,4-D + 0,5 mg L-1 BAP dengan inkubasi dalam keadaan 24 jam gelap (perlakuan 1) dan MS + 1,5 mg L-1 2,4-D + 1,0 mg L-1 BAP dengan fotoperiode 16/8 jam (perlakuan 2), selama 28 minggu. Kemudian, respons regenerasi kalus menjadi tunas diuji dengan penanaman kalus pada media regenerasi (MS + 3,4 mg L-1 BAP + 0,09 mg L-1 NAA) selama 12 minggu. Hasil penelitian menunjukkan bahwa eksplan pada perlakuan 2 lebih responsif untuk menginduksi kalus dari sisik umbi L. longiflorum dibandingkan eksplan pada perlakuan 1. Kalus yang dihasilkan bertekstur kompak dan berwarna hijau kekuningan dengan tingkat kesintasan 100% dan daya proliferasi yang tinggi. Media regenerasi berhasil meregenerasikan kalus menjadi tunas sebesar 100%, meskipun tidak terdapat pertumbuhan akar dalam penelitian ini. Perlakuan MS + 1,5 mg L-1 2,4-D + 1,0 mg L-1 BAP dengan fotoperiode 16/8 jam direkomendasikan sebagai sebuah protokol yang efektif dalam pengembangan L. longiflorum.

show abstract

“…The plants produce distinctive aromatic flowers and can be grown in multiple climatic conditions in the world (Askari et al, 2018;Youssef et al, 2019). Due to its great importance, it has been used for a long time to make bouquets (Alam et al, 2013), used as potted plants, decorating homes, and as cut flowers that last a long time (Bhandari and Aswath, 2018), and it also has a great export value . Historically, the majority of lily species have been employed as culinary or medicinal plants (Tang et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Supplementing Organo-Bio-Stimulants Into Growing Media Improves Growth, Biochemical Response, Flower Productivity and Quality of Lily Bulbs

Sewedan,

Youssef

2023

Scient. J. of Flowers and Ornament. Plants

View full text Add to dashboard Cite

Addition of organo-bio-stimulants may have major impact on bio-chemical attributes of growing media and flower productivity. The current study evaluated the response of lily bulbs for growth, some bio-chemical characteristics, and flowering quality to various growing media which included sand, clay, sand: clay (1:1 v/v), or sand: clay: compost (1:1:1 v/v) supplemented with Humicil and/or Power max. Results proved that the use of growing media led to significant differences in growth, flowering, and chemical component parameters of lily plants. However, there was superiority in these traits due to planting in a medium consisting of a mixture of sand: clay: compost (1:1:1 v/v), followed by sand: clay (1:1 v/v), then clay media. On contrast, planting in sand soil was coincided with a decrease in above-mentioned characteristics compared to the other media. The addition of various organo-bio-stimulants coincided with an improvement in growth, flowering and chemical component measurements. Moreover, adding the mixture of Humicil with Power max, followed by Power max alone showed the greatest effect on improving measurements. The combination between growing media and stimulants showed a significant difference in all measurements of plant growth, flowering and chemical components of lily plants. However, the highest values of these traits coincided with the combination between a mixture of sand: clay: compost (1:1:1 v/v) with the mixture of Humicil and Power max.

show abstract

Standardization of an Effective Protocol for in vitro Culture of Lilium longiflorum Thunb cv. Pavia

Cited by 4 publications

References 5 publications

Standardization of in vitro micropropagation procedure of Oriental Lilium Hybrid Cv. ‘Ravenna’

Standardization of in vitro micropropagation procedure of Oriental Lilium Hybrid Cv. ‘Ravenna’

Induksi Kalus Sisik Umbi Lilium longiflorum Thunb. oleh Auksin dan Sitokinin, serta Respons Pertumbuhannya Secara In Vitro

Supplementing Organo-Bio-Stimulants Into Growing Media Improves Growth, Biochemical Response, Flower Productivity and Quality of Lily Bulbs

Contact Info

Product

Resources

About