Standardization of Eclipta Alba by HPTLC, HPLC and AAS

Bhattacharjee, Arka; Chakraborty, Arpan; Dutta, Debanchal; A, Sau; Chakraborty, Saikat; Samanta, Nilanjan; Ps, Biswas; Mukhopadhyay, Goutam

doi:10.4172/2153-2435.1000541

Cited by 4 publications

(3 citation statements)

References 4 publications

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“…HPTLC method was used to quantify the β-sitosterol reported by Bhattacharjee et al (2017) with minor modifications [23]. The CAMAG HPTLC system consisting of WINCATS software, LINOMAT V automatic sample applicator, an automatic development chamber, scanning densitometer CAMAG scanner 3, and photo documentation apparatus CAMAG reprostar 3 were used.…”

Section: Standardization and Quantification Of β-Sitosterol In Hair G...mentioning

confidence: 99%

“…HPLC method was performed to quantify the β-sitosterol reported by Bhattacharjee et al (2017) with some modifications [23]. RP-HPLC system (Shimadzu Prominence, Kyoto, Japan) equipped with two Shimadzu LC-20 UFLC reciprocating pumps, a variable Shimadzu SPD-M20A Prominence PDA detector and a rheodyne manual injector with a loop size of 20 μl was used.…”

Section: Standardization and Quantification Of β-Sitosterol In Hair G...mentioning

confidence: 99%

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Process Validation of Beta-Sitosterol Hair Gel Formulation and Evaluation of 5 Alpha Reductase Inhibition in Vitro for the Treatment of Androgenetic Alopecia

Chakraborty

Bhattacharjee

CHAKRABORTY³

et al. 2023

Int J App Pharm

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Objective: The present study was aimed to develop topical gel containing β-sitosterol using carbopol 940 as a gelling agent and to investigate 5 alpha reductase (5α-reductase) inhibitory activity of suitable gel formulation and compare it with a commercial product used topically for alopecia. Methods: Three different batches of β-sitosterol hair gel formulation were manufactured and evaluated. Additionally, the 5α-reductase inhibitory activity of the prepared formulation, finasteride as a positive control, was evaluated and compared to the commercial herbal formulation used. Results: According to the analytical findings of three different batches, the gel formulation is good in appearance, homogeneous, and easily spreadable. Based on findings from HPLC and HPTLC, the amount of β-sitosterol in those formulations complies with the label claim. By checking different critical parameters of those batches, we established the manufacturing process method validation and the process reproducibility. In vitro results showed the good 5α-reductase inhibitory potential of prepared gel formulation and then commercial product. The IC50 value of the prepared formulation was 118.960±0.634 (µg/ml) and standard beta-sitosterol 88.854±0.70 (µg/ml), whereas Finasteride (positive control) 224.372±3.103 (ng/ml). Conclusion: Thus, β-sitosterol formulation utilises a straightforward, low-cost production, less time-consuming process with minimal facility and equipment requirements. The formulation may be a promising candidate for future investigation into their antiandrogenic activities.

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Section: Standardization and Quantification Of β-Sitosterol In Hair G...mentioning

confidence: 99%

Section: Standardization and Quantification Of β-Sitosterol In Hair G...mentioning

confidence: 99%

Process Validation of Beta-Sitosterol Hair Gel Formulation and Evaluation of 5 Alpha Reductase Inhibition in Vitro for the Treatment of Androgenetic Alopecia

Chakraborty

Bhattacharjee

CHAKRABORTY³

et al. 2023

Int J App Pharm

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“…12 Standardization of β-sitosterol in E. alba Extracts using HPTLC The approach described by Bhattacharjee et al (2017) has been modified in some respects. 13 CAMAG HPTLC system includes the scanning densitometer, LINOMAT V automated sample applicator, and automatic development chamber, with WINCATS software. The image documentation tools CAMAG Reprostar 3 and CAMAG Scanner 3 were utilized.…”

Section: Qualitative Evaluation Of E Alba Extractsmentioning

confidence: 99%

Exploring the Potential of Eclipta alba: A Promising Approach for Hair Treatment Management through 5-Alpha Reductase Inhibition

Chakraborty¹,

Bhattacharjee²,

Mondal³

et al. 2023

IJPQA

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A class of medications known as 5 alpha reductase (5α-reductase or 5αR) inhibitors is used to treat male pattern hair loss and benign prostatic hyperplasia. This study shows that Eclipta alba has 5αR inhibitory action that is helpful in the treatment of androgenic diseases. For 5αR enzyme inhibition evaluation, E. alba was extracted using methanol and petroleum ether. Further phytochemical screening can be done. Phytosterols test negatively found in methanol extract during phytochemical screening but positive in petroleum ether extract of E. alba. HPTLC data of different extracts was performed based on the phytochemical screening found. According to the HPTLC analysis, petroleum ether extract of E. alba contained 0.11% of β-sitosterol, while the methanolic extract had a higher concentration of 4.75%. The inhibitory activity of these plant extracts against 5αR was examined in comparison to the commonly used 5αR inhibitor, finasteride. IC50 measurements for petroleum ether extract of E. alba and β-sitosterol (a chemical biomarker derived from the plant material) were established as 150.76 ± 4.56 and 77.09 ± 3.07 μg/mL, correspondingly. These results indicate their potential as compelling contenders worthy of deeper exploration regarding their anti-androgenic properties. The notable abundance of β-sitosterol in the petroleum ether extract of E. alba enhances its potential for significant biological activity, particularly in terms of inhibiting the 5αR enzyme.

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A high-performance thin-layer chromatography method for the simultaneous determination of quercetin and gallic acid in Eclipta alba and Guiera senegalensis

Khalid

Alqarni

Foudah

et al. 2021

JPC-J Planar Chromat

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Standardization of Eclipta Alba by HPTLC, HPLC and AAS

Cited by 4 publications

References 4 publications

Process Validation of Beta-Sitosterol Hair Gel Formulation and Evaluation of 5 Alpha Reductase Inhibition in Vitro for the Treatment of Androgenetic Alopecia

Process Validation of Beta-Sitosterol Hair Gel Formulation and Evaluation of 5 Alpha Reductase Inhibition in Vitro for the Treatment of Androgenetic Alopecia

Exploring the Potential of Eclipta alba: A Promising Approach for Hair Treatment Management through 5-Alpha Reductase Inhibition

A high-performance thin-layer chromatography method for the simultaneous determination of quercetin and gallic acid in Eclipta alba and Guiera senegalensis

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