2014
DOI: 10.1016/j.aquaculture.2014.01.020
|View full text |Cite
|
Sign up to set email alerts
|

Standardization of fertilization protocols for the European eel, Anguilla anguilla

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
77
0
1

Year Published

2015
2015
2024
2024

Publication Types

Select...
6
2
1

Relationship

2
7

Authors

Journals

citations
Cited by 81 publications
(78 citation statements)
references
References 28 publications
0
77
0
1
Order By: Relevance
“…At the facility, eels were acclimatized to saltwater (36‰) and temperature (20°C) in 300 L tanks in RAS systems. The experimental protocol was established according to Butts et al (2014). Thus, female eels received weekly injections of salmon pituitary extract (SPE Argent Chemical Laboratories, Washington, USA) at 18.75 mg / kg body weight for four months to induce vitellogenesis, followed by one dihydroxyprogesterone (17a,20ß-dihydroxy-4-pregnen-3-one; Sigma-Aldrich Denmark A/S) injection at 2 mg per/kg body weight to induce final oocyte maturation and ovulation.…”
Section: Experimental Maturationmentioning
confidence: 99%
“…At the facility, eels were acclimatized to saltwater (36‰) and temperature (20°C) in 300 L tanks in RAS systems. The experimental protocol was established according to Butts et al (2014). Thus, female eels received weekly injections of salmon pituitary extract (SPE Argent Chemical Laboratories, Washington, USA) at 18.75 mg / kg body weight for four months to induce vitellogenesis, followed by one dihydroxyprogesterone (17a,20ß-dihydroxy-4-pregnen-3-one; Sigma-Aldrich Denmark A/S) injection at 2 mg per/kg body weight to induce final oocyte maturation and ovulation.…”
Section: Experimental Maturationmentioning
confidence: 99%
“…Sperm concentration was measured using spermatocrit (Haematokrit 210, Andreas Hettich GmbH & Co. KG, Tuttlingen, Germany), obtained from spinning milt for 10 min at 6000 g (Sørensen et al, 2013). After motility analyses, milt was diluted in artificial seminal plasma, P1 medium, described by Asturiano et al (2014) (Butts et al, 2014) at a ratio of 1:99. Diluted milt was kept in sterile culture flasks at 20˚C, prior to fertilization (within 2 h post-stripping).…”
Section: Broodstock and Gamete Managementmentioning
confidence: 99%
“…Later, from 2010 to 2014, the European project PRO-EEL (Reproduction of European eel: towards a self-sustained aquaculture) was performed in order to expand the knowledge base on European eel reproduction and to develop standardised protocols for the controlled production of viable eggs and the culture of larvae. Within the framework of this European project, the Towkiewicz group from Denmark was able to produce larvae, which remained alive for up to 22 days (Butts et al, 2014). Also, Pérez et al from the Aquaculture and Biodiversity of Valencia group (GAB) obtained the first fertilization, hatching and larvae in Spain .…”
Section: Eel Reproduction In Captivitymentioning
confidence: 99%