Standardized hot water extract from the leaves of <i>Hydrangea serrata</i> (Thunb.) Ser. alleviates obesity <i>via</i> the AMPK pathway and modulation of the gut microbiota composition in high fat diet-induced obese mice

Han, Hee-Soo; Lee, Hwi Ho; Gil, Hyo Sun; Chung, Kwansoo; Kim, Jeon Kyung; Kim, Dong Hyun; Yoon, Jimin; Chung, Eun Kyoung; Lee, Jong Kil; Yang, Woong Mο; Shin, Yeonjong; Ahn, Hye Shin; Lee, Sun Hee; Lee, Kyung Tae

doi:10.1039/d0fo02185g

Cited by 18 publications

(23 citation statements)

References 50 publications

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“…Ser. (WHS) using high-fat diet (HFD)induced obese mice and found that WHS may exert anti-obesity effects [23]. In addition to poor diet, genetic factors can contribute to the development of obesity [24]; thus, we further expanded our investigation on the weight loss effect of daily intake of WHS and the underlying molecular mechanisms in genetically obese db/db mice in this study.…”

Section: Introductionmentioning

confidence: 95%

“…Total cellular and tissue protein were extracted as previously described [23]. The protein concentration was quantified by Bradford assay (Bio-Rad Laboratories, Inc., Hercules, CA, USA) according to the manufacturer's instructions.…”

Section: Protein Extraction and Western Blotmentioning

confidence: 99%

“…Oil Red O staining was conducted as described previously [23]. Briefly, 3T3-L1 cells were fully differentiated in the presence or absence of WHS as described above.…”

Section: Oil Red O Stainingmentioning

confidence: 99%

“…A part of subcutaneous fat and liver were fixed with 4% buffered formaldehyde and stained with hematoxylin and eosin (H&E) as described previously [23]. The stained slides were analyzed under a microscope (Olympus, Tokyo, Japan).…”

Section: Histological Analysismentioning

confidence: 99%

“…The equal concentrations of purified products were pooled together and subjected to pyrosequencing using Illumina iSeq 100 Sequencing System (Illumina, San Diego, CA, USA). The detailed procedures were described previously [23].…”

Section: Microbiome Sampling and Microbiome Taxonomic Profiling (Mtp)mentioning

confidence: 99%

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Standardized Hydrangea serrata (Thunb.) Ser. Extract Ameliorates Obesity in db/db Mice

Han

Chung

Shin³

et al. 2021

Nutrients

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We previously reported the potential anti-obesity effects of the water extract of Hydrangea serrata (Thunb.) Ser. leaves (WHS) in high-fat diet-induced obese mice. As an extension of our previous study, we investigated the anti-adipogenic and anti-obesity effects of WHS and its underlying molecular mechanisms in 3T3-L1 preadipocytes and genetically obese db/db mice. WHS attenuated the gene expression of adipogenic transcription factors, CCAAT/enhancer binding protein (C/EBP)α, peroxisome proliferator-activated receptor (PPAR)γ, and sterol regulatory element binding protein (SREBP)-1. Moreover, WHS inhibited the mitotic clonal expansion of preadipocytes by inducing G1 cell cycle arrest. Oral administration of WHS alleviated body weight gain and body fat accumulation in vivo. In addition, adipocyte hypertrophy and liver steatosis were ameliorated by WHS treatment. WHS reduced C/EBPα, PPARγ, and SREBP-1 expression and activated AMPKα phosphorylation in both white adipose tissue (WAT) and liver tissue. WHS also mildly upregulated the expression of thermogenic proteins, including uncoupling protein-1, PPARs, PPARγ coactivator-1α, and sirtuin-1, in brown adipose tissue (BAT). Furthermore, WHS altered the gut microbiota composition to resemble that of wild-type mice. Taken together, our findings suggest that WHS could alleviate adiposity by inhibiting adipogenesis in WAT and the liver and modulating the gut microbiota.

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Section: Introductionmentioning

confidence: 95%

Section: Protein Extraction and Western Blotmentioning

confidence: 99%

“…Oil Red O staining was conducted as described previously [23]. Briefly, 3T3-L1 cells were fully differentiated in the presence or absence of WHS as described above.…”

Section: Oil Red O Stainingmentioning

confidence: 99%

Section: Histological Analysismentioning

confidence: 99%

Section: Microbiome Sampling and Microbiome Taxonomic Profiling (Mtp)mentioning

confidence: 99%

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Standardized Hydrangea serrata (Thunb.) Ser. Extract Ameliorates Obesity in db/db Mice

Han

Chung

Shin³

et al. 2021

Nutrients

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Yellow leaf green tea modulates the AMPK/ACC/SREBP1c signaling pathway and gut microbiota in high‐fat diet‐induced mice to alleviate obesity

Tian,

Pan,

Zhou

et al. 2024

J Sci Food Agric

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BackgroundYellow leaf green tea (YLGT) is a new variety of Camellia sinensis (L.) O. Ktze, which has yellow leaves and the unique qualities of ‘three green through three yellow’. This study aimed to investigate the anti‐obesity effect of YLGT in mice fed a high‐fat diet (HFD) and to explore the potential mechanisms by regulating the AMPK/ACC/SREBP1c signaling pathways and gut microbiota.ResultsThe results showed that YLGT aqueous extract reduced body weight, hepatic inflammation, fat accumulation and hyperlipidemia in HFD‐induced C57BL/6J mice, and accelerated energy metabolism, reduced fat synthesis, and suppressed obesity by activating the AMPK/CPT‐1α signaling pathway and inhibiting the FAS/ACC/SREBP‐1c signaling pathway. Fecal microbiota transplantation experiment further confirmed that the alteration of gut microbiota, e.g., increasing unclassified_Muribaculaceae and decreasing Colidextribacter, might be an important cause of YLGT water extract inhibiting obesity.ConclusionIn conclusion, YLGT has a broad application prospect in the treatment of obesity and the development of anti‐obesity function beverages.This article is protected by copyright. All rights reserved.

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Flammulina velutipes mycorrhizae dietary fiber improves lipid metabolism disorders in obese mice through activating AMPK signaling pathway mediated by gut microbiota

et al. 2021

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Standardized hot water extract from the leaves of Hydrangea serrata (Thunb.) Ser. alleviates obesity via the AMPK pathway and modulation of the gut microbiota composition in high fat diet-induced obese mice

Abstract: WHS prevented the HFD-induced obesity via regulating AMPK and AKT/mTOR signaling pathway. WHS might be a potential dietary supplement for alleviating obesity.

Cited by 18 publications

References 50 publications

Standardized Hydrangea serrata (Thunb.) Ser. Extract Ameliorates Obesity in db/db Mice

Standardized Hydrangea serrata (Thunb.) Ser. Extract Ameliorates Obesity in db/db Mice

Yellow leaf green tea modulates the AMPK/ACC/SREBP1c signaling pathway and gut microbiota in high‐fat diet‐induced mice to alleviate obesity

Flammulina velutipes mycorrhizae dietary fiber improves lipid metabolism disorders in obese mice through activating AMPK signaling pathway mediated by gut microbiota

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