Staphylococcal Resistance Patterns, blaZ and SCCmec Cassette Genes in the Nasopharyngeal Microbiota of Pregnant Women

Abstract

Antimicrobial resistance in Staphylococcus spp. colonising the nasopharynx can create risk factors of therapeutic treatment failure or prophylaxis in pregnant women. Resistance is mostly encoded on plasmids (e.g., blaZ gene for penicillinase synthesis) or chromosomes (e.g., mecA and mecC for methicillin resistance). The mecA gene is part of the chromosomal mec gene cassette (SCCmec), which is also located on the plasmid. The disc diffusion method for the selected drugs (beta-lactams, fluoroquinolones, streptogramins, aminoglicosides, macrolides, oxasolidinones, tetracyclines and other groups) was used. PCR for blaZ, mecA and mecC genes and SCCmec cassette detection and typing were performed. S. aureus (54.4%) and S. epidermidis (27.9%) were the most prevalent and showed the highest diversity of resistance profiles. The blaZ, mecA and mecC genes were reported in 95.6%, 20.6% and 1.5% of isolates, respectively. The highest resistance was found to beta-lactams, commonly used during pregnancy. Resistance to a variety of antimicrobials, including benzylpenicillin resistance in blaZ-positive isolates, and the existence of a very high diversity of SCCmec cassette structures in all staphylococci selected from the nasopharyngeal microbiota of pregnant women were observed for the first time. Knowledge of the prevalence of antimicrobial-resistant staphylococci in the nasopharynx of pregnant women may be important for the appropriate treatment or prophylaxis of this group of patients.

Methicillin-resistant coagulase-positive staphylococci in new, middle-aged, and old veterinary hospitals in southern Thailand: A preliminary study

“…Less than half of the isolated pathogens carried the mecA gene, whereas the majority of CoPS isolates contained the blaZ gene, consistent with previous reports [ 1 , 46 ]. Bacterial isolates with the mecA and blaZ genes in veterinary hospitals are related to those in human hospitals [ 47 , 48 ]. Beta-lactam antibiotics and trimethoprim/sulfonamide are the antimicrobial classes to which resistance is most often encountered [ 1 , 46 ], similar to a previous study by Ogunrinu et al .…”

Pendekatan One Health: Deteksi Gen blaZ dari Isolat Staphylococcus aureus Asal Peternakan Sapi Perah di Sulawesi Selatan

“…Gen blaZ dari isolat S. aureus pada ternak sapi perah di Kenya dilaporkan sebanyak (41,1%) sedangkan pada manusia gen blaZ dari isolat S. aureus dilaporkan sebanyak 51,5%. Keberadaan gen blaZ dapat menimbulkan ancaman terhadap pengobatan dan penanganan mastitis pada ternak sapi dan kesehatan manusia (Mbindyo et al, 2021;Andrzejczuk et al, 2023). Penyebaran resistansi penisilin terutama terjadi melalui penyebaran strain resistan (Lowy, 2003).…”