Staphylococcus aureus from 152 cases of bovine, ovine and caprine mastitis investigated by Multiple-locus variable number of tandem repeat analysis (MLVA)

Abstract

Staphylococcus aureus is one of the main etiological agents of mastitis in ruminants. In the present retrospective study, we evaluated the potential interest of a previously described automated multiple loci Variable Number of Tandem Repeats (VNTR) Assay (MLVA) comprising 16 loci as a first line tool to investigate the population structure of S. aureus from mastitis. We determined the genetic diversity of S. aureus strains from cases of clinical and subclinical mastitis in dairy cattle (n = 118, of which 16 were methicillin-resistant), sheep (n = 18) and goats (n = 16). The 152 strains could be subdivided into 115 MLVA genotypes (including 14 genotypes for the ovine strains and 15 genotypes for the caprine strains). This corresponds to a discriminatory index (D) value of 0.9936. Comparison with published MLVA data obtained using the same protocol applied to strains from diverse human and animal origins revealed a low number (8.5%) of human-related MLVA genotypes among the present collection. Eighteen percent of the S. aureus mastitis collection belonged to clonal complexes apparently not associated with other pathological conditions. Some of them displayed a relatively low level of diversity in agreement with a restricted ecological niche. These findings provide arguments suggesting that specific S. aureus lineages particularly adapted to ruminant mammary glands have emerged and that MLVA is a convenient tool to provide a broad overview of the population, owing to the availability via internet of databases compiling published MLVA genotypes.Electronic supplementary materialThe online version of this article (doi:10.1186/s13567-014-0097-4) contains supplementary material, which is available to authorized users.

Phenotypic and genotypic characterization of Staphylococcus aureus isolates in milk from flocks diagnosed with subclinical mastitis

“…They include methods of comparative typing based on standard electrophoretic bands, typing methods of libraries and genome sequencing. Among the typing methods based on fingerprint, PCR-RFLP, RAPD-PCR, ERIC-PCR, BOX-PCR, and REP-PCR have been described as simple and quick techniques for taxonomic and epidemiological analyses of several species of the genus Staphylococcus (Bergonier et al, 2014;Idil and Bilkay, 2014;Rahman et al, 2016;Ławniczek-Wałczyk et al, 2017). Epidemiological molecular studies have contributed significantly to the understanding of the sources, routes of transmission and prognosis for many pathogens that cause bovine mastitis, as well as to understand the mechanisms of adaptation to the host and the causes of the disease (Supré et al, 2011;McMillan et al, 2016).…”

Puerarin Exerts an Antiinflammatory Effect by Inhibiting NF-kB and MAPK Activation inStaphylococcus aureus-Induced Mastitis

“…S. aureus is one of the most prevalent etiologic agents in bovine mastitis (Bergonier et al, 2014), which leads to an inflammatory disease characterized by congestion, hardness of the udder, and formation of milk clots (Alluwaimi et al, 2003). Mammary epithelial cells play a key role as defenders (Gilbert et al, 2013).…”

Isolation and Molecular Identification of Staphylococcus Spp. From Bovine Mastitis Milk Samples.

“…In a view of this, the emergence of methicillin resistance among Staphylococcus spp. isolated from bovine milk has been reported in different countries and continents (Bergonier et al 2014). …”

“…A DNA marker of known DNA fragment sizes (500 bp ladder) was run alongside the specimens to aid in identification of the products. Desired amplified product was cut from the gel and purified using QIAGEN gel purification kit (QIAGEN, Germany) (Bergonier et al 2014). …”