Staphylococcus aureus from atopic dermatitis skin alters cytokine production triggered by monocyte-derived Langerhans cell

Iwamoto, Kazumasa; Moriwaki, Masaya; Niitsu, Yoshie; Saino, M.; Takahagi, Shunsuke; Hisatsune, Junzo; Sugai, Motoyuki

doi:10.1016/j.jdermsci.2017.08.001

Cited by 35 publications

(37 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We show that LCs respond to S. aureus with production of a Th1/Th17-polarizing cytokine profile. These data corroborate finding by others, who have demonstrated that LCs internalize S. aureus and subsequently polarize T cells towards Th17 (Igyártó et al, 2011, Kobayashi et al, 2015, van der Aar et al, 2013, Iwamoto et al, 2017. We now elucidate that detection of S. aureus WTA β-GlcNAc is of critical importance for the induced response.…”

Section: Discussionsupporting

confidence: 92%

Staphylococcus aureuswall teichoic acid is a pathogen-associated molecular pattern that is recognized by langerin (CD207) on skin Langerhans cells

Diaz

Rumpret

et al. 2017

Preprint

View full text Add to dashboard Cite

SummaryStaphylococcus aureus is a major cause of skin and soft tissue infections and aggravator of the inflammatory skin disease atopic dermatitis (AD). Langerhans cells (LCs) initiate a Th17 response upon exposure to S. aureus, which contributes to host defense but also to AD pathogenesis. However, the molecular mechanisms underlying the unique pro-inflammatory capacities of S. aureus remain unclear. We demonstrate that human LCs directly interact with S. aureus through the pattern-recognition receptor langerin (CD207), which specifically recognizes the conserved β-N-acetylglucosamine (GlcNAc) modifications of wall teichoic acid (WTA) that are not expressed by other staphylococcal species. The WTA glycoprofile strongly influences the production of Th1-and Th17-polarizing cytokines by LCs. Specifically, β-GlcNAc activates LCs, whereas co-decoration of WTA with α-GlcNAc through the enzyme TarM, uniformly present in the AD-associated CC1 lineage, attenuates LC immune activation. Our findings provide important mechanistic insights into the role of S. aureus in inflammatory skin disease.

show abstract

Section: Discussionsupporting

confidence: 92%

Staphylococcus aureuswall teichoic acid is a pathogen-associated molecular pattern that is recognized by langerin (CD207) on skin Langerhans cells

Diaz

Rumpret

et al. 2017

Preprint

View full text Add to dashboard Cite

show abstract

“…Staphylococcus aureus is the most common cutaneous pathogen capable of evading host defense . S. aureus residing inside human keratinocytes was associated with atopic dermatitis, while S. aureus colonization in chronic diabetic foot ulcers contributes to impaired DNA repair mechanisms, which subsequently serves as a facilitator for the persistent, unresolved inflammatory state found in these wounds .…”

Section: Discussionmentioning

confidence: 99%

“…However, the presence of bacterial infection often impedes wound closure and barrier restoration . Staphylococcus aureus is the most common pathogen causing wound infections and is capable of evading host defense . While S. aureu s is the prevalent pathogen in multiple cutaneous conditions including non‐healing wounds and atopic dermatitis, the mechanisms by which this pathogen persists in skin infection remain largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Single cell analyses reveal specific distribution of anti‐bacterial molecule Perforin‐2 in human skin and its modulation by wounding and Staphylococcus aureus infection

Štrbo

Pastar

Romero

et al. 2019

Experimental Dermatology

View full text Add to dashboard Cite

Perforin‐2 (P‐2) is a recently described antimicrobial protein with unique properties to kill intracellular bacteria. We investigated P‐2 expression pattern and cellular distribution in human skin and its importance in restoration of barrier function during wound healing process and infection with the common wound pathogen Staphylococcus aureus. We describe a novel approach for the measurement of P‐2 mRNA within individual skin cells using an amplified fluorescence in situ hybridization (FISH) technique. The unique aspect of this approach is simultaneous detection of P‐2 mRNA in combination with immune‐phenotyping for cell surface proteins using fluorochrome‐conjugated antibodies. We detected P‐2 transcript in both hematopoietic (CD45+) and non‐hematopoietic (CD45−) cutaneous cell populations, confirming the P‐2 expression in both professional and non‐professional phagocytes. Furthermore, we found an induction of P‐2 during wound healing. P‐2 overexpression resulted in a reduction of intracellular S. aureus, while infection of human wounds by this pathogen resulted in P‐2 suppression, revealing a novel mechanism by which S. aureus may escape cutaneous immunity to cause persistent wound infections.

show abstract

“…While IL-1 produced by phagocytes is an important cytokine orchestrating host defense against S. aureus , IL-1β is an important proinflammatory cytokine that activates monocytes, macrophages, and neutrophils [39-42]. S. aureus from atopic dermatitis skin can alter cytokine production triggered by monocyte-derived Langerhans cells [43]. …”

Section: Skin Immune Systemmentioning

confidence: 99%

<b><i>Staphylococcus aureus</i></b> and Host Immunity in Recurrent Furunculosis

Nowicka

Grywalska

2019

Dermatology

View full text Add to dashboard Cite

Staphylococcus aureus is one of the severest and most persistent bacterial pathogens. The most frequent S. aureus infections include impetigo, folliculitis, furuncles, furunculosis, abscesses, hidradenitis suppurativa, and mastitis. S. aureus produces a great variety of cellular and extracellular factors responsible for its invasiveness and ability to cause pathological lesions. Their expression depends on the growth phase, environmental factors, and location of the infection. Susceptibility to staphylococcal infections is rooted in multiple mechanisms of host immune responses and reactions to bacterial colonization. Immunological and inflammatory processes of chronic furunculosis are based on the pathogenicity of S. aureus as well as innate and acquired immunity. In-depth knowledge about them may help to discover the whole pathomechanism of the disease and to develop effective therapeutic options. In this review, we focus on the S. aureus-host immune interactions in the pathogenesis of recurrent furunculosis according to the most recent experimental and clinical findings.

show abstract

Staphylococcus aureus from atopic dermatitis skin alters cytokine production triggered by monocyte-derived Langerhans cell

Abstract: S. aureus TF3378 on AD skin can skew T cell responses via LC toward imbalanced Th1/Th2 skin immunity.

Cited by 35 publications

References 40 publications

Staphylococcus aureuswall teichoic acid is a pathogen-associated molecular pattern that is recognized by langerin (CD207) on skin Langerhans cells

Staphylococcus aureuswall teichoic acid is a pathogen-associated molecular pattern that is recognized by langerin (CD207) on skin Langerhans cells

Single cell analyses reveal specific distribution of anti‐bacterial molecule Perforin‐2 in human skin and its modulation by wounding and Staphylococcus aureus infection

<b><i>Staphylococcus aureus</i></b> and Host Immunity in Recurrent Furunculosis

Contact Info

Product

Resources

About