Staphylococcus aureus induces IL-1β expression through the activation of MAP kinases and AP-1, CRE and NF-κB transcription factors in the bovine mammary gland epithelial cells

Kim, Kyoung Whun; Im, Jino; Jeon, Jun Ho; Lee, Hong-Gu; Yun, Cheol‐Heui; Han, Seung Hyun

doi:10.1016/j.cimid.2011.04.004

Cited by 29 publications

(20 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We did not detect a significant change in IL-1β gene expression in the bMECs challenged with live S. aureus (MOI 30:1, 2 h) (Figure 6A), but the secretion of IL-1β in the S. aureus -challenged bMECs decreased (Figure 6B). Opposite to our results, Kim et al (2011) showed that the level of IL-1β mRNA and the cytokine secretion were up-regulated in a bovine mammary epithelial cell line (MAC-T cell) stimulated with heat-killed S. aureus (10 8 CFU/ml) for 3 h. The 0.25 mM NaO treatment slightly decreased the IL-1β secretion, and the infection alone did not change this effect. A similar result was observed at the mRNA level (Figure 6).…”

Section: Discussioncontrasting

confidence: 79%

“…In this sense, the antimicrobial response of bMECs treated with 1 mM NaO (24 h) is more effective than in 0.25 mM NaO-treated cells. We analyzed the gene expression of cytokines and chemokines in bMECs that might be modulated by S. aureus challenge (Lahouassa et al, 2007; Kim et al, 2011; Schukken et al, 2011; Alva-Murillo et al, 2014b). IL-1β is a pro-inflammatory cytokine that plays a critical role in the host defense against infection (Bannerman, 2009; Günther et al, 2011), and together with TNF-α is responsible for early inflammatory responses (Günther et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Sodium Octanoate Modulates the Innate Immune Response of Bovine Mammary Epithelial Cells through the TLR2/P38/JNK/ERK1/2 Pathway: Implications during Staphylococcus aureus Internalization

Alva-Murillo

Ochoa‐Zarzosa

López‐Meza

2017

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Bovine mammary epithelial cells (bMECs) contribute to mammary gland defense against invading pathogens, such as Staphylococcus aureus (intracellular facultative), which is recognized by TLR2. In a previous report, we showed that sodium octanoate [NaO, a medium chain fatty acid (C8)] induces (0.25 mM) or inhibits (1 mM) S. aureus internalization into bMECs and differentially regulates the innate immune response (IIR). However, the molecular mechanisms have not been described, which was the aim of this study. The results showed that α5β1 integrin membrane abundance (MA) was increased in 0.25 mM NaO-treated cells, but TLR2 or CD36 MA was not modified. When these receptors were blocked individually, 0.25 mM NaO-increased S. aureus internalization was notably reduced. Interestingly, in this condition, the IIR of the bMECs was impaired because MAPK (p38, JNK, and ERK1/2) phosphorylation and the activation of transcription factors related to these pathways were decreased. In addition, the 1 mM NaO treatment induced TLR2 MA, but neither the integrin nor CD36 MA was modified. The reduction in S. aureus internalization induced by 1 mM NaO was increased further when TLR2 was blocked. In addition, the phosphorylation levels of the MAPKs increased, and 13 transcriptional factors related to the IIR were slightly activated (CBF, CDP, c-Myb, AP-1, Ets-1/Pea-3, FAST-1, GAS/ISRE, AP-2, NFAT-1, OCT-1, RAR/DR-5, RXR/DR-1, and Stat-3). Moreover, the 1 mM NaO treatment up-regulated gene expression of IL-8 and RANTES and secretion of IL-1β. Notably, when 1 mM NaO-treated bMECs were challenged with S. aureus, the gene expression of IL-8 and IL-10 increased, while IL-1β secretion was reduced. In conclusion, our results showed that α5β1 integrin, TLR2 and CD36 are involved in 0.25 mM NaO-increased S. aureus internalization in bMECs. In addition, 1 mM NaO activates bMECs via the TLR2 signaling pathways (p38, JNK, and ERK1/2), which improves IIR before S. aureus invasion. Additionally, NaO (1 mM) might exert anti-inflammatory effects after bacterial internalization.

show abstract

Section: Discussioncontrasting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Sodium Octanoate Modulates the Innate Immune Response of Bovine Mammary Epithelial Cells through the TLR2/P38/JNK/ERK1/2 Pathway: Implications during Staphylococcus aureus Internalization

Alva-Murillo

Ochoa‐Zarzosa

López‐Meza

2017

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…* P <0.05 is significantly different from LPS. [26]. Additional studies are necessary to develop new prophylactic and therapeutic approaches for mastitis and have been performed in the mouse model.…”

Section: Discussionmentioning

confidence: 98%

Bergenin Plays an Anti-Inflammatory Role via the Modulation of MAPK and NF-κB Signaling Pathways in a Mouse Model of LPS-Induced Mastitis

et al. 2014

View full text Add to dashboard Cite

Mastitis is a major disease in humans and other animals and is characterized by mammary gland inflammation. It is a major disease of the dairy industry. Bergenin is an active constituent of the plants of genus Bergenia. Research indicates that bergenin has multiple biological activities, including anti-inflammatory and immunomodulatory properties. The objective of this study was to evaluate the protective effects and mechanism of bergenin on the mammary glands during lipopolysaccharide (LPS)-induced mastitis. In this study, mice were treated with LPS to induce mammary gland mastitis as a model for the disease. Bergenin treatment was initiated after LPS stimulation for 24 h. The results indicated that bergenin attenuated inflammatory cell infiltration and decreased the concentration of NO, TNF-α, IL-1β, and IL-6, which were increased in LPS-induced mouse mastitis. Furthermore, bergenin downregulated the phosphorylation of nuclear factor-kappaB (NF-κB) and mitogen-activated protein kinases (MAPK) signaling pathway proteins in mammary glands with mastitis. In conclusion, bergenin reduced the expression of NO, TNF-α, IL-1β, and IL-6 proinflammatory cytokines by inhibiting the activation of the NF-κB and MAPKs signaling pathways, and it may represent a novel treatment strategy for mastitis.

show abstract

“…The present result also showed that the expression of OPN were up-regulated concomitantly with an increase in NF-kB activity in vivo and in vitro. In the bovine mammary gland epithelial cells, Staphylococcus aureus also induces IL-1beta expression through the activation of NF-kB transcription factors [28]. It is not surprising that NF-kB is active in many inflammatory diseases because NF-kB controls many genes involved in inflammation.…”

Section: Discussionmentioning

confidence: 99%

LPS induces pro-inflammatory response in mastitis mice and mammary epithelial cells: Possible involvement of NF-κB signaling and OPN

Xiao

Wang

Liu

et al. 2015

Pathologie Biologie

View full text Add to dashboard Cite

Staphylococcus aureus induces IL-1β expression through the activation of MAP kinases and AP-1, CRE and NF-κB transcription factors in the bovine mammary gland epithelial cells

Cited by 29 publications

References 41 publications

Sodium Octanoate Modulates the Innate Immune Response of Bovine Mammary Epithelial Cells through the TLR2/P38/JNK/ERK1/2 Pathway: Implications during Staphylococcus aureus Internalization

Sodium Octanoate Modulates the Innate Immune Response of Bovine Mammary Epithelial Cells through the TLR2/P38/JNK/ERK1/2 Pathway: Implications during Staphylococcus aureus Internalization

Bergenin Plays an Anti-Inflammatory Role via the Modulation of MAPK and NF-κB Signaling Pathways in a Mouse Model of LPS-Induced Mastitis

LPS induces pro-inflammatory response in mastitis mice and mammary epithelial cells: Possible involvement of NF-κB signaling and OPN

Contact Info

Product

Resources

About