Staphylococcus Aureus Ve Koagülaz Negatif Stafilokokların Vankomisin Ve Teikoplanin Duyarlılıklarının Araştırılmasında Otomatize Sistem İle E-Test Yönteminin Karşılaştırılması

Abstract

Objective Staphylococcus are the leading agent of treatment difficulties in both community-acquired and hospital-acquired infections. The formation of methicillin resistance has made the use of glycopeptide antibiotics compulsory in the treatment. Glycopeptide antibiotics have been widely used in methicillin-resistant Staphylococcal infections, but the rapid and accurate identification of the susceptibility of these antibiotics is of great importance, since the susceptibility of intermediate susceptible and resistant strains to glycopeptides has begun to be known in recent years.Materials and Methods In our study, susceptibilities of vancomycin and teicoplanin in 100 Staphylococcus aureus and 100 coagulase negative Staphylococcus strains isolated from various clinical samples sent to Kahramanmaras Sutcu Imam University Medical Faculty Hospital Medical Microbiology Laboratory were investigated by VITEK-2 (BioMeriéux, France) automated system and E-test method. The sensitivities of antibiotics were evaluated according to CLSI criteria.Results All strains were found sensitive to vancomycin and teicoplanin by e-test method. According to the automated system, all strains were susceptible to vancomycin, whereas one MRSA strain was resistant, one MRCNS strain was intermediate susceptible, and seven MSCNS were intermediate susceptible.Conclusion According to our study, glycopeptide antibiotics can still be safely used in the treatment of methicillin-resistant Staphylococcus. However, the use of these antibiotics should be cautious because of the reporting of indermetidate susceptible and resistant strains. For this reason, it is of great importance to determine the most appropriate methods for accurate and rapid determination of glycopeptide antibiotic susceptibility. ( Sakarya Med J 2017, 7(2):92-96 )