Staphylococcus borealis sp. nov., isolated from human skin and blood

Pain, Maria Ronessen; Wolden, Runa; Jaén‐Luchoro, Daniel; Salvà‐Serra, Francisco; Piñeiro-Iglesias, Beatriz; Karlsson, Roger; Klingenberg, Claus; Cavanagh, Jorunn Pauline

doi:10.1099/ijsem.0.004499

Cited by 28 publications

(20 citation statements)

References 54 publications

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“…haemolyticus have been reclassified as Staph. borealis [ 29 ]. Therefore, molecular techniques may have limitations including insufficient discriminatory power in the case of closely related species or the lack of quality of sequences deposited in the GenBank [ 30 ], as also observed in this work for gap gene sequencing.…”

Section: Discussionmentioning

confidence: 99%

Species identification by MALDI-TOF MS and gap PCR–RFLP of non-aureus Staphylococcus, Mammaliicoccus, and Streptococcus spp. associated with sheep and goat mastitis

Rosa

Penati

Fusar-Poli

et al. 2022

Vet Res

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Staphylococci and streptococci are common causes of intramammary infection in small ruminants, and reliable species identification is crucial for understanding epidemiology and impact on animal health and welfare. We applied MALDI-TOF MS and gap PCR–RFLP to 204 non-aureus staphylococci (NAS) and mammaliicocci (NASM) and to 57 streptococci isolated from the milk of sheep and goats with mastitis. The top identified NAS was Staphylococcus epidermidis (28.9%) followed by Staph. chromogenes (27.9%), haemolyticus (15.7%), caprae, and simulans (6.4% each), according to both methods (agreement rate, AR, 100%). By MALDI-TOF MS, 13.2% were Staph. microti (2.9%), xylosus (2.0%), equorum, petrasii and warneri (1.5% each), Staph. sciuri (now Mammaliicoccus sciuri, 1.0%), arlettae, capitis, cohnii, lentus (now M. lentus), pseudintermedius, succinus (0.5% each), and 3 isolates (1.5%) were not identified. PCR–RFLP showed 100% AR for Staph. equorum, warneri, arlettae, capitis, and pseudintermedius, 50% for Staph. xylosus, and 0% for the remaining NASM. The top identified streptococcus was Streptococcus uberis (89.5%), followed by Strep. dysgalactiae and parauberis (3.5% each) and by Strep. gallolyticus (1.8%) according to both methods (AR 100%). Only one isolate was identified as a different species by MALDI-TOF MS and PCR–RFLP. In conclusion, MALDI-TOF MS and PCR–RFLP showed a high level of agreement in the identification of the most prevalent NAS and streptococci causing small ruminant mastitis. Therefore, gap PCR–RFLP can represent a good identification alternative when MALDI-TOF MS is not available. Nevertheless, some issues remain for Staph. haemolyticus, minor NAS species including Staph. microti, and species of the novel genus Mammaliicoccus.

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Section: Discussionmentioning

confidence: 99%

Species identification by MALDI-TOF MS and gap PCR–RFLP of non-aureus Staphylococcus, Mammaliicoccus, and Streptococcus spp. associated with sheep and goat mastitis

Rosa

Penati

Fusar-Poli

et al. 2022

Vet Res

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“…felis (). Although, 16S rRNA gene sequences often lack sufficient variation to differentiate between species of the genus Staphylococcus in pairwise comparisons [8–11]. Indeed, neither H8/1 T nor H16/1A T could be differentiated from S.…”

Section: Full-textmentioning

confidence: 99%

“…2). Although, 16S rRNA gene sequences often lack sufficient variation to differentiate between species of the genus Staphylococcus in pairwise comparisons [8][9][10][11]. Indeed, neither H8/1 T nor H16/1A T could be differentiated from S. devriesei or S. felis type strains respectively on the basis of 16 rRNA gene sequence, Table 1.…”

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confidence: 99%

Staphylococcus caledonicus sp. nov. and Staphylococcus canis sp. nov. isolated from healthy domestic dogs

Newstead

Harris

Goodbrand

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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Two strains, H8/1T and H16/1AT, of Gram-stain-positive, coagulase-negative staphylococci were isolated from separate healthy domestic dogs in Scotland. Both strains were genome sequenced and their inferred DNA–DNA hybridisation indicates that H8/1T and H16/1AT represent two novel species of the genus Staphylococcus . On the basis of the results of genome sequence analysis (genome blast distance phylogeny and single nucleotide polymorphism analysis) H8/1T is most closely related to Staphylococcus devriesei and H16/1AT most closely related to Staphylococcus felis . Also, average nucleotide identity distinguished H8/1T and H16/1AT from S. devriesei and S. felis as did minor phenotypic differences. On the basis of these results, it is proposed that H8/1T and H16/1AT represent novel species with the respective names Staphylococcus caledonicus and Staphylococcus canis. The type strain of S. caledonicus is H8/1T (=NCTC 14452T=CCUG 74789T). The type strain of S. canis is H16/1AT (=NCTC 14451T=CCUG 74790T)

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“…Device-related infections are one of the most common manifestations of CoNS infections, causing high morbidity and mortality [ 6 , 7 ]. With the advent of modern tools in routine microbiological diagnostics such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), pathogen detection has experienced a remarkable refinement within the past decade [ 8 , 9 ]. However, reliable species identification by MALDI-TOF MS relies greatly on the quality and accuracy of the employed database.…”

Section: Introductionmentioning

confidence: 99%

Staphylococcus massiliensis isolated from human blood cultures, Germany, 2017–2020

Last

Lepper

Jung

et al. 2022

Eur J Clin Microbiol Infect Dis

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Clinical and laboratory data on newly described staphylococcal species is rare, which hampers decision-making when such pathogens are detected in clinical specimens. Here, we describe Staphylococcus massiliensis detected in three patients at a university hospital in southwest Germany. We report the discrepancy of microbiological findings between matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, 16S-rRNA polymerase chain reaction, and whole-genome sequencing for all three isolates. Our findings highlight the diagnostic pitfalls pertinent to novel and non-model organisms in daily microbiological practice, in whom the correct identification is dependent on database accuracy.

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Staphylococcus borealis sp. nov., isolated from human skin and blood

Cited by 28 publications

References 54 publications

Species identification by MALDI-TOF MS and gap PCR–RFLP of non-aureus Staphylococcus, Mammaliicoccus, and Streptococcus spp. associated with sheep and goat mastitis

Species identification by MALDI-TOF MS and gap PCR–RFLP of non-aureus Staphylococcus, Mammaliicoccus, and Streptococcus spp. associated with sheep and goat mastitis

Staphylococcus caledonicus sp. nov. and Staphylococcus canis sp. nov. isolated from healthy domestic dogs

Staphylococcus massiliensis isolated from human blood cultures, Germany, 2017–2020

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