Staphylococcus lugdunensis small colony variant conversion resulting in chronic prosthetic joint infection

Dahyot, Sandrine; Lebeurre, Jérémie; Hansmann, Yves; Oustau, C. Ronde; Prévost, Gilles

doi:10.1016/j.medmal.2017.05.005

Cited by 10 publications

(11 citation statements)

References 8 publications

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“…Staphylococcus lugdunensis is responsible for infections ranging from minor skin and soft tissue infections (Bocher et al, 2009; Arias et al, 2010) to invasive diseases such as infective endocarditis (Non and Santos, 2017), bone and joint infections (Douiri et al, 2016; Lourtet-Hascoët et al, 2016; Argemi et al, 2017a), prosthetic device-infections (Anguera, 2005; Shah et al, 2010) and vascular catheter-related infections (Ebright et al, 2004), both in healthy host and immune-compromised patients. One of the characteristics of S. lugdunensis infections is the development of deep-seated abscess lesions that cannot be cleared without surgical intervention associated with effective antibiotic therapy (Arias et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Comparative Genome Analysis of Staphylococcus lugdunensis Shows Clonal Complex-Dependent Diversity of the Putative Virulence Factor, ess/Type VII Locus

et al. 2019

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Staphylococcus lugdunensis is a commensal bacterium of human skin that has emerged as a virulent Coagulase-Negative Staphylococcus in both community-acquired and healthcare associated infections. Genotyping methods have shown a clonal population structure of this pathogen but failed to identify hypervirulent lineages. Here, complete genomes of three pathogenic and three carriage S. lugdunensis strains were obtained by Single-Molecule sequencing (PacBio) and compared to 15 complete genomes available in GenBank database. The aim was to identify (i) genetic determinants specific to pathogenic or carriage strains or specific to clonal complexes (CCs) defined by MultiLocus Sequence Typing, and (ii) antibiotic resistance genes and new putative virulence factors encoded or not by mobile genetic elements (MGE). Comparative genomic analysis did not show a strict correlation between gene content and the ability of the six strains to cause infections in humans and in a Galleria mellonella infection model. However, this study identified new MGEs (five prophages, two genomic islands and one plasmid) and genetic variations of some putative virulence-associated loci, especially in CC3 strains. For a clonal population, high variability and eight CC-dependent genetic organizations were observed for the ess locus, which encodes a putative type VII secretion system (T7SS) homologous to that of S. aureus. Further phenotypic and functional studies are needed to characterize this particular CC3 and to evaluate the role of T7SS in the virulence of S. lugdunensis.

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Section: Introductionmentioning

confidence: 99%

Comparative Genome Analysis of Staphylococcus lugdunensis Shows Clonal Complex-Dependent Diversity of the Putative Virulence Factor, ess/Type VII Locus

et al. 2019

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“…Staphylococcus lugdunensis is a coagulase-negative Staphylococcus (CoNS) belonging to the normal human skin microbiota 1 , 2 that is increasingly recognized as a virulent pathogen in both community-acquired and healthcare associated infections 3 , 4 . This commensal bacterium has mainly been associated with serious infections, such as skin and soft tissue infections 5 , 6 , bone and joint infections 7 , 8 , but also bacteremia and endocarditis 9 , 10 . Thus, the pathogenicity of S .…”

Section: Introductionmentioning

confidence: 99%

Multiple-Locus Variable Number Tandem Repeat Analysis (MLVA) and Tandem Repeat Sequence Typing (TRST), helpful tools for subtyping Staphylococcus lugdunensis

Dahyot¹,

Lebeurre

François

et al. 2018

Sci Rep

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Staphylococcus lugdunensis is an emergent virulent coagulase-negative Staphylococcus that is increasingly responsible for severe infections. In an attempt to generate informative sequence data for subtyping S. lugdunensis, we selected and sequenced seven polymorphic variable number of tandem repeats (VNTRs) to develop two new methods: a classic length-based multiple-locus VNTR analysis (MLVA) method and a tandem repeat sequence typing (TRST) method. We assessed their performances compared to two existing methods, multilocus sequence typing (MLST) and multivirulence-locus sequence typing (MVLST) for 128 isolates from diverse clinical settings and geographical origins. The clustering achieved by the four methods was highly congruent, with MLVA discriminating within clonal complexes as defined by MLST. Indeed, MLVA was highly discriminant compared to MLST and MVLST in terms of number of genotypes as well as diversity indexes. Sequencing of the seven VNTRs showed that they were stable, and analysis of sequence polymorphisms provided superior discriminatory power. The typeability, reproducibility, and epidemiological concordance of these new methods were excellent. Of note, no link between clustering and clinical settings was identified. This study demonstrates that MLVA and TRST provide valuable information for molecular epidemiological study of S. lugdunensis, and represent promising tools to distinguish between strains of homogenous lineages in this clonal species.

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“…More recently, a case of normal variant S lugdunensis PJI who was initially treated by DAIR, developed a persistent infection 1 year later, and SCVs were isolated [30]. This highlights the importance of not underestimating the pathogenicity of S lugdunensis .…”

Section: Discussionmentioning

confidence: 99%

“…[29]28(58-78)13 M, 15 F4 CVD, 2 cancer, 1 DM, 1 rheumatoid disease3-56 wk16 TKR, 10 THR, 1 foot, 1 shoulderArgemi et al. [30]170F2 yTKRCVD, cardiovascular disease; DM, diabetes mellitus; F, female; HBV, hepatitis B; M, male; MG, myasthenia gravis; No., number of patients; THR, total hip replacement; TKR, total knee replacement.…”

Section: Discussionmentioning

confidence: 99%

Small-colony variant of Staphylococcus lugdunensis in prosthetic joint infection

2018

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Prosthetic joint infection is usually caused by staphylococci. Among the coagulase-negative staphylococci, Staphylococcus lugdunensis is important because it behaves as a pathogen similar to S aureus. It also develops biofilms, and the biofilm phenotype can appear as small-colony variants. Although genetically indistinguishable, they differ in size and antibiotic susceptibility from the parent strain and are responsible for chronic persistent infection and failure of antibiotic treatment. They can also lead to misinterpretation of results. The patient reported here underwent total knee replacement and 2 years later presented with prosthetic joint infection. Tissue samples and prosthesis taken at revision grew S lugdunensis, the majority of which were small-colony variants. Recommendations are made for their detection and identification.

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Staphylococcus lugdunensis small colony variant conversion resulting in chronic prosthetic joint infection

Cited by 10 publications

References 8 publications

Comparative Genome Analysis of Staphylococcus lugdunensis Shows Clonal Complex-Dependent Diversity of the Putative Virulence Factor, ess/Type VII Locus

Comparative Genome Analysis of Staphylococcus lugdunensis Shows Clonal Complex-Dependent Diversity of the Putative Virulence Factor, ess/Type VII Locus

Multiple-Locus Variable Number Tandem Repeat Analysis (MLVA) and Tandem Repeat Sequence Typing (TRST), helpful tools for subtyping Staphylococcus lugdunensis

Small-colony variant of Staphylococcus lugdunensis in prosthetic joint infection

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