Starch accumulation is associated with active growth in A. tequilana

Zavala-García, Laura E.; Sánchez-Segura, Lino; Dios, Emmanuel Ávila de; Pérez-López, Arely V.; Simpson, June

doi:10.1016/j.plaphy.2018.08.011

Cited by 10 publications

(11 citation statements)

References 35 publications

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“…Transcriptome analysis of the vegetative to reproductive transition in A. tequilana revealed no differential expression for starch metabolism related genes (Zavala- Garcia et al, 2018) whereas fructan related genes are highly expressed in SAM tissue in comparison to leaf tissue. In particular a 6G-FFT isoform is specifically and strongly expressed at the initial stage of the reproductive phase (Avila De Dios et al, 2019).…”

Section: Agave Fructansmentioning

confidence: 91%

“…In Agave leaves starch accumulation is largely limited to stomatal guard cells with minimal accumulation in other leaf cells (Zavala- Garcia et al, 2018). The presence of oligofructans containing 3-5 fructan residues (3-5 degrees of polymerization, D.P.)…”

Section: Agave Fructansmentioning

confidence: 99%

“…Oligofructans and/or sucrose transported from leaves are either metabolized to starch that accumulates in the peripheral meristem region between the leaf base and the stem (Zavala- Garcia et al, 2018; needed whereas degradation of fructans is carried out by fructan exohydrolases (FEH) that may be specific for β 1→2 or β 1→6 linkages or act on both. FT and FEH in common with vacuolar and cell wall invertases are members of Plant Glycoside Hydrolase Family 32 (PGHF32).…”

Section: Agave Fructansmentioning

confidence: 99%

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The Sweet Taste of Adapting to the Desert: Fructan Metabolism in Agave Species

Pérez-López

Simpson

2020

Front. Plant Sci.

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Over 70% of Agave species, (159 of 206) are found in Mexico and are well adapted to survive under hot, arid conditions, often in marginal terrain, due to a unique combination of morphological and physiological attributes. In the pre-Columbian era agaves were also key to human adaptation to desert terrain. In contrast to other species such as cacti or resurrection plants, Agaves store carbohydrates in the form of fructan polymers rather than starch or sucrose, however, properties specific to fructans such as a strong hydration shell, the ability to be transported through phloem, variable composition throughout the Agave life-cycle and accumulation in succulent tissues and flowers suggest a potential for multiple functional roles. This mini-review summarizes current knowledge of molecular and biochemical aspects of fructan metabolism in Agave species.

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Section: Agave Fructansmentioning

confidence: 91%

Section: Agave Fructansmentioning

confidence: 99%

Section: Agave Fructansmentioning

confidence: 99%

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The Sweet Taste of Adapting to the Desert: Fructan Metabolism in Agave Species

Pérez-López

Simpson

2020

Front. Plant Sci.

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“…This hypothesis is supported by recent observations using terahertz spectroscopy that show a large hydration capacity for agave fructans 13 . In contrast starch is thought to accumulate only transiently in specific tissues 14 and has not been associated with tolerance to dehydration. In order to explore the association between carbohydrates and water content in A. victoriae-reginae leaves, transverse sections of white basal leaf tissue were stained by either PAS (an non-specific stain for carbohydrates) or lugol (a specific stain for starch).…”

Section: Resultsmentioning

confidence: 99%

“…Following the sectioning, samples were drawn in 1 mL of toluidine blue solution (0.05% w/v in phosphate buffer 1 M at pH 6.8) for 3 min, then washed for 1 min in deionized water and visualized under bright field microscopy (OLYMPUS BX60 microscope and Leica E24HD stereoscope). To identify total carbohydrates on fresh tissue sections, Periodic Acid-Schiff (PAS) and Lugol staining were carried out and visualized as described in 14 .…”

Section: Methods Plant Materialmentioning

confidence: 99%

Three-dimensional water mapping of succulent Agave victoriae-reginae leaves by terahertz imaging

Singh

Pérez-López²,

Simpson³

et al. 2020

Sci Rep

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While terahertz imaging has been used before for the determination of water content in vegetative tissue, most studies have either presented measurements of the temporal evolution of water content at a single-point of the plant or have presented two-dimensional images of leaves, demonstrating the potential of the technique, but relatively little of such information has been used to support biologically relevant conclusions. in this article we introduce terahertz time-domain spectroscopic imaging as a technique for the determination of the three-dimensional distribution of water in succulent plant tissues. We present the first three-dimensional water mapping of an agave leaf, which demonstrates an unprecedented capability to study the water retention mechanisms within succulent plants. We found that agave leaves are composed of a low-hydration outer tissue layer, defined by the outermost layer of vascular tissue that surrounds a high-hydration tissue, the carbohydrate rich hydrenchyma. The findings are supported by histological images and the correlation between the water content and carbohydrate presence is consistent with recently published findings of a remarkably large hydration shell associated with agave fructans.

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