Starch phosphorylation and the in vivo regulation of starch metabolism and characteristics

You, Yuxian; Zhang, Mingyue; Yang, Wen; Cheng, Li; Liu, Yuntao; Li, Caiming; He, Jiyu; Wu, Wenjing

doi:10.1016/j.ijbiomac.2020.05.156

Cited by 13 publications

(7 citation statements)

References 65 publications

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“…As important modulators of protein function, cysteine modifications such as S -nitrosylation, S -sulfenylation and S -glutathionylation have received increasing attention in plants ( 7–9 ). Plant PTMs play critical roles in nearly all biological processes, such as signal transduction ( 4 , 10 ), metabolism ( 11 , 12 ), stress resistance ( 13 , 14 ), plant immunity ( 15 , 16 ) and other cellular events.…”

Section: Introductionmentioning

confidence: 99%

qPTMplants: an integrative database of quantitative post-translational modifications in plants

Han

Wang

Cao

et al. 2021

Nucleic Acids Research

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As a crucial molecular mechanism, post-translational modifications (PTMs) play critical roles in a wide range of biological processes in plants. Recent advances in mass spectrometry-based proteomic technologies have greatly accelerated the profiling and quantification of plant PTM events. Although several databases have been constructed to store plant PTM data, a resource including more plant species and more PTM types with quantitative dynamics still remains to be developed. In this paper, we present an integrative database of quantitative PTMs in plants named qPTMplants (http://qptmplants.omicsbio.info), which hosts 1 242 365 experimentally identified PTM events for 429 821 nonredundant sites on 123 551 proteins under 583 conditions for 23 PTM types in 43 plant species from 293 published studies, with 620 509 quantification events for 136 700 PTM sites on 55 361 proteins under 354 conditions. Moreover, the experimental details, such as conditions, samples, instruments and methods, were manually curated, while a variety of annotations, including the sequence and structural characteristics, were integrated into qPTMplants. Then, various search and browse functions were implemented to access the qPTMplants data in a user-friendly manner. Overall, we anticipate that the qPTMplants database will be a valuable resource for further research on PTMs in plants.

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Section: Introductionmentioning

confidence: 99%

qPTMplants: an integrative database of quantitative post-translational modifications in plants

Han

Wang

Cao

et al. 2021

Nucleic Acids Research

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“…Therefore, α-amylases with adoptions to high, medium, and low temperatures were invented via genetic engineering and bio-engineering. The maximum utilization of starch needs the combination of carbohydrases targeting specific metabolites under certain environmental conditions, instead of the effects of individuals [ 28 ]. In the present study, we used Box Behnken design to predict the optimistic combination of glucoamylase, pullulanase, maltase, thermostable α-amylase, medium temperature α-amylase, and cold-active α-amylase for starch digestion, represented by RSA and improved DMD, in diets for broilers aged 1 to 3 wk.…”

Section: Discussionmentioning

confidence: 99%

Optimization of exogenous carbohydrases supplemented in broiler diets using in vitro simulated gastrointestinal digestion and response surface methodology

Liu

Liu²,

Jiang

et al. 2021

PLoS ONE

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The present study aimed to explore the optimal zymogram of combination of 6 carbohydrases (glucoamylase, pullulanase, maltase, thermostable α-amylase, medium temperature α-amylase, and cold-active α-amylase) supplemented in corn-soybean based diet of broilers aged 1 to 3 wk for the maximum starch digestibility, by using in vitro simulated gastrointestinal digestion and response surface method. The third generation of simulated monogastric animal digestion system was used for in vitro digestion experiment. By using single factor completely random design, the optimal supplement levels of single carbohydras were determined by the reducing sugar release amount and improved dry matter digestibility, which were the parameters representing the starch digestibility of the diet. Additionally, Box-Behnken response surface method was used to predict the optimal combination of 6 carbohydrases. The results showed that the optimistic zymogram of 6 carbohydrases in corn-soybean based diet for broilers aged 1 to 3 wk were 297.39 U/g glucoamylase, 549.72 U/g pullulanase, 3.01 U/g maltase, 1,455.73 U/g thermostable α-amylase, 278.64 U/g medium temperature α-amylase, and 1,985.97 U/g cold-active α-amylase, and the associated reduced sugar release amount and improved dry matter digestibility were 215.98 mg/g, and 6.23%, respectively. Furthermore, we conducted in vitro digestion experiments with diets supplemented with the predicted optimistic zymogram and found that the experimental reduced sugar release amount and improved dry matter digestibility were 219.26 mg/g and 6.31% respectively, whose errors to the predicted optimistic reducing sugar release amount and the improved dry matter digestibility were 1.05% and 1.02%. To sum up, the predicted optimal zymogram of 6 carbohydrases in the present study were capable to improve the starch digestibility in diet for broilers aged 1 to 3 wk, which were represented by increased reduced sugar release amount and improved dry matter digestibility.

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“…Phosphorylation helps to improve the physiochemical properties of starch. Phosphorylation amount varies based on organ of plant and species [92]. Starch biosynthetic protein complexes were initially identified in the endosperm of wheat and were later found in wheat leaves [30].…”

Section: Regulation Of Protein Phosphorylationmentioning

confidence: 99%

Soluble Starch Synthase Enzymes in Cereals: An Updated Review

et al. 2021

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Cereal crops have starch in their endosperm, which has provided calories to humans and livestock since the dawn of civilization to the present day. Starch is one of the important biological factors which is contributing to the yield of cereal crops. Starch is synthesized by different enzymes, but starch structure and amount are mainly determined by the activities of starch synthase enzymes (SS) with the involvement of starch branching enzymes (SBEs) and debranching enzymes (DBEs). Six classes of SSs are found in Arabidopsis and are designated as soluble SSI-V, and non-soluble granule bound starch synthase (GBSS). Soluble SSs are important for starch yield considering their role in starch biosynthesis in cereal crops, and the activities of these enzymes determine the structure of starch and the physical properties of starch granules. One of the unique characteristics of starch structure is elongated glucan chains within amylopectin, which is by SSs through interactions with other starch biosynthetic enzymes (SBEs and DBEs). Additionally, soluble SSs also have conserved domains with phosphorylation sites that may be involved in regulating starch metabolism and formation of heteromeric SS complexes. This review presents an overview of soluble SSs in cereal crops and includes their functional and structural characteristics in relation to starch synthesis.

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Starch phosphorylation and the in vivo regulation of starch metabolism and characteristics

Cited by 13 publications

References 65 publications

qPTMplants: an integrative database of quantitative post-translational modifications in plants

qPTMplants: an integrative database of quantitative post-translational modifications in plants

Optimization of exogenous carbohydrases supplemented in broiler diets using in vitro simulated gastrointestinal digestion and response surface methodology

Soluble Starch Synthase Enzymes in Cereals: An Updated Review

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