STAT3 Knockdown Induces Tumor Formation by MDA-MB-231 Cells

Banerjee, Kasturi; Pru, Cindy A.; Pru, James K.; Resat, Haluk

doi:10.31487/j.cor.2018.10.002

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…Western blot analysis. Western blot analysis was performed to measure the protein levels of relative mRNAs in PBMCs from patients with VIT or healthy controls as previously described (21). Proteins were lysed and extracted using RIPA buffer (P0013C; Beyotime Institute of Biotechnology) shortly after cell collection, and protein concentration was measured using a BCA protein kit (Sigma-Aldrich; Merck KGaA).…”

Section: Rna Isolation and Reverse Transcription-quantitative (Rt-q)mentioning

confidence: 99%

MicroRNA‑21‑5p protects melanocytes via targeting STAT3 and modulating Treg/Teff balance to alleviate vitiligo

Huo

Liu

et al. 2020

Mol Med Rep

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Vitiligo (VIT) is caused by loss and degradation of functional epidermal melanocytes. Studies have indicated that melanocyte destruction may be associated with an imbalance between regulatory T cells (Treg cells) and effector T cells (Teff cells). The current study aimed to investigate the molecular mechanism through which Treg/Teff balance affects VIT pathogenesis. To explore this, peripheral blood mononuclear cells were isolated from patients with VIT and healthy individuals. The present study revealed that the proportions of CD4 + T cells, Treg cells and T helper 1 (Th1) cells were decreased in patients with VIT, but those of Teff cells (Th17 and Th22 cells) were increased; additionally, Foxp3 expression was decreased, but the expression levels of interferon-γ, interleukin (IL)-17A and IL-22 were increased. Furthermore, in patients with VIT, microRNA (miR)-21-5p expression was decreased, while that of STAT3 was increased. Further in vitro experiments in CD4 + T cells revealed that STAT3 was targeted by miR-21-5p. Functional analysis further indicated that miR-21-5p overexpression in Th17-polarized CD4 + T cells decreased the proportion of Teff cells and associated cytokines, such as IL-17A and IL-22, but increased the proportion of Treg cells and Foxp3. However, the effects of miR-21-5p overexpression were partly reversed by STAT3 overexpression. Increased apoptosis of melanocytes was detected after co-culture with Th17-polarized CD4 + T cells in the presence of a miR-21-5p mimic. However, this indirect effect of the miR-21-5p mimic on melanocytes was decreased via STAT3 overexpression. Therefore, miR-21-5p may protect melanocytes via targeting STAT3 and regulating Treg/Teff balance. The current findings may provide a possible treatment method for managing VIT.

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Section: Rna Isolation and Reverse Transcription-quantitative (Rt-q)mentioning

confidence: 99%

MicroRNA‑21‑5p protects melanocytes via targeting STAT3 and modulating Treg/Teff balance to alleviate vitiligo

Huo

Liu

et al. 2020

Mol Med Rep

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“…e eight included articles [25][26][27][28][29][30][31][32] were published between 2010 and 2017 in China, and all were case-control studies. Figure 1 shows a flowchart of the literature screening process.…”

Section: Literature Search Resultsmentioning

confidence: 99%

“…However, established opinions on the tumor-promoting effects of STAT3 have been frequently challenged. Banerjee et al [25] found that STAT3 has a dual role in breast cancer since STAT3 promotes breast cancer under certain conditions, but it may also be a negative regulator of certain types of breast cancer.…”

Section: Discussionmentioning

confidence: 99%

Role of STAT3 Expression in Thyroid Cancer: A Meta-Analysis and Systematic Review Based on the Chinese Population

Liang

Zhang

Song

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Signal transduction and activator of transcription 3 (STAT3) is an oncogene with transcriptional activity. In recent years, there have been several studies concerning the clinicopathological significance of the expression of the STAT3 protein in thyroid cancer. However, the results are still inconsistent. In this study, we conducted a meta-analysis to evaluate the relationship between the expression of STAT3 protein and thyroid cancer susceptibility and its clinicopathological characteristics. Methods. We searched the China National Knowledge Infrastructure (CNKI) database, Chinese Biomedical Literature Database (CBM), Chinese Scientific and Journal Database (VIP), Wanfang, PubMed, and EMBASE. The time frame of the publication search was from the establishment of each of the databases until December 2021. We performed a meta-analysis to quantitatively evaluate the relationship between the expression of the STAT3 protein in thyroid cancer and its clinicopathological characteristics. Results. A total of eight articles were included in the meta-analysis, covering 448 thyroid cancer patients and 227 controls. Results indicated that the expression of STAT3 protein in thyroid cancer tissue is highly expressed (OR = 14.41, 95% CI (6.94, 29.91), p < 0.001). Besides, we also discovered that STAT3 protein is negatively correlated with thyroid cancer tumor diameter and TNM stage (OR = 0.13, 95% CI (0.05, 0.33), p < 0.001; OR = 0.40, 95% CI (0.24, 0.67), p < 0.001) and positively correlated with lymph node metastasis (OR = 2.83, 95% CI (1.08, 7.46), p = 0.035). However, STAT3 expression is not related to gender (OR = 0.88, 95% CI (0.54, 1.44), p = 0.609), age (OR = 0.54, 95% CI (0.21, 1.36), p = 0.191), capsular invasion (OR = 2.98, 95% CI (0.23, 38.29), p = 0.403), or tumor multiplicity (OR = 0.25, 95% CI (0.003, 19.28), p = 0.533). Conclusions. This study reveals that STAT3 protein expression is significantly related to the susceptibility and clinicopathological characteristics of thyroid cancer. It also suggests that STAT3 may be a potential predictor of the clinical progression of thyroid cancer.

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Cysteine cathepsins L and X differentially modulate interactions between myeloid-derived suppressor cells and tumor cells

Jakoš

Pišlar

Fonović

et al. 2020

Cancer Immunol Immunother

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STAT3 Knockdown Induces Tumor Formation by MDA-MB-231 Cells

Cited by 6 publications

References 25 publications

MicroRNA‑21‑5p protects melanocytes via targeting STAT3 and modulating Treg/Teff balance to alleviate vitiligo

MicroRNA‑21‑5p protects melanocytes via targeting STAT3 and modulating Treg/Teff balance to alleviate vitiligo

Role of STAT3 Expression in Thyroid Cancer: A Meta-Analysis and Systematic Review Based on the Chinese Population

Cysteine cathepsins L and X differentially modulate interactions between myeloid-derived suppressor cells and tumor cells

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