State of endothelial function, lipid spectrum and features of coronary vessels structure of rats with obesity and insulin resistance under iodine deficiency conditions

Todoriv, T. V.; Bagriy, Mykola; Voronych-Semchenko, N. M.

doi:10.15407/fz67.06.021

Cited by 2 publications

(3 citation statements)

References 12 publications

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“…Тварини 4-6-ї груп масою 150 -165 г перебували в умовах високофруктозного вигодовування (отримували замість питної води 20%-й розчин фруктози; «Голден Фарм», Україна) впродовж 8 тиж [13]. Тварини 2-ї та 5-ї груп (покоління, яке народжене після природного спарювання від щурів, які перебували на йододефіцитній дієті не менше ніж 2 міс, включаючи гестаційний період самиць)отримували дієту з обмеженим вмістом йоду від народження; 3-ї та 6-ї -отримували йододефіцитну дієту у статевозрілому віці впродовж 2 міс [18]. Щури контрольної групи знаходилися на стандартному харчовому раціоні віварію з вільним доступом до звичайної води.…”

Section: методикаunclassified

“…3). Зростання активності АсАТ у тварин на тлі висококалорійної дієти може відображати метаболічну гіперактивність клітин для забезпечення фізіологічних функцій, зважаючи на участь амінотрансферази у трансмембранному транспорті відновних потенціалів через мембрану мітохондрій та регенерацію НАДН [18,20].…”

Section: результати та їх обговоренняunclassified

“…У тварин із ІР на тлі постнатального йододефіциту розбіжності досліджуваних показників щодо ізольованих ІР та монойододефіциту були виражені менше. Загалом підвищення вмісту досліджуваних ферментів у сироватці крові щурів 5-ї та 6-ї дослідних груп відображає вираженість цитолітичного синдрому, високу ймовірність ускладненого перебігу та несприятливого прогнозу за умов коморбідної патології, зокрема, розвитку глікогенової гепатопатії [18,25]. Значення коефіцієнту де-Рітіса в інсулінорезистентних тварин за умов належного та обмеженого забезпечення йодом можуть відображати розвиток нетоксичного стеатозу та стеатогепатиту, що особливо важливо у разі поєднаної патології.…”

Section: результати та їх обговоренняunclassified

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Predictors of Hepatocytes Cytolysis of Insulin Resistant Rats Under Conditions of Pre- And Postnatal Iodine Deficiency

Stetseviat¹,

Voronych-Semchenko²,

Bahrii³

et al. 2023

Fiziol Zh

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Carbohydrate metabolism markers, thyroid profile indexes, transaminase activity and structural features of liver were studied, taking into account sexual dimorphism, in rats which were keeping on a high-fructose diet under conditions of adequate iodine supply and pre- and postnatal iodine deficiency. It was found that under fructose loading of animals with iodine deficiency the risk of insulin resistance (IR) development increases (HOMA-IR index in insulin-resistant male rats under conditions of pre- and postnatal iodine deficiency increases respectively by 22 and 13% compared to the data in animals that received a high-carbohydrate diet and adequate iodine supply). It is important, that in males and females with IR, the secretion of free triiodothyronine decreased by 36 and 40%, thyroid-stimulating hormone – by 28 and 34%, respectively, compared to the data of intact animals. The development of IR was accompanied by the activation of aminotransferases in blood serum (more in males). In particular, in male rats with IR under conditions of pre- and postnatal iodine deficiency, the activity of aspartate aminotransferase increased by 2.7 and 2.4 times, alanine aminotransferase – by 3.9 and 2.7 times, respectively, which led to a decrease of the de-Ritis index (more significantly against the background of prenatal iodine deficiency – by 25%) in relation to the data in animals that were on a standard diet. The activity of aminotransferases in rats with IR under conditions of iodine deprivation was higher than in animals with isolated iodine deficiency – by 11-56% and IR – by 13-47 %. In animals with IR, the presence of binucleated hepatocytes with a diffuse decrease in the number of glycogen granules in them and accumulation of glycoproteins in the wall of liver vessels were found. The foci of fatty dystrophy and pronounced zonal character of dystrophic processes in hepatocytes were identified in females, diffuse dystrophy prevailed in males. In the cytoplasm of hepatocytes of animals with IR under conditions of prenatal iodine deficiency, the lightening and the granularity, single granules of glycogen were observed. Under these conditions, the average perimeter (by 74%) and area (by three times) of hepatocytes, the average area of nucleus (by 3.1 times), the average perimeter (by 50%) and area (by 66%) of the nucleolus were increased compared to the control values. The structural changes were consistent with an increase of the transaminase activity. Thus, fructose loading and the development of IR acts as a trigger for cytolysis of hepatocytes, which increases under conditions of iodine deficiency, especially prenatal.

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Section: методикаunclassified

Section: результати та їх обговоренняunclassified

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Predictors of Hepatocytes Cytolysis of Insulin Resistant Rats Under Conditions of Pre- And Postnatal Iodine Deficiency

Stetseviat¹,

Voronych-Semchenko²,

Bahrii³

et al. 2023

Fiziol Zh

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Changes of endothelial function and oxidant status in insulin resistance and obesity under the conditions of iodine deficiency

Todoriv

Voronych-Semchenko

Didushko

2023

Mìžnarodnij endokrinologìčnij žurnal

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Background. The topicality of the theme is due to the significant prevalence of cardiovascular diseases and an increase in costs for medical care, the high risk of disability, which characterizes the medical and social component of the problem. Pathological changes can be a manifestation not only of a cardiovascular disorder, but also develop as a result of other diseases, including diabetes mellitus, obesity, and thyroid pathology. One of the main mechanisms of morbidity and mortality is macrovascular complications that can occur in endothelial dysfunction and oxidative stress. The purpose of the research is to study the peculiarities of changes in the parameters of the endothelial system and oxidant status in animals with insulin resistance and obesity under conditions of adequate iodine supply and iodine deficiency. Materials and methods. Study included 75 sexually mature rats having received a high-carbohydrate, high-fat diet under conditions of adequate and limited iodine supply, followed by analysis of markers of carbohydrate metabolism, thyroid status, indices of endothelial function, lipid peroxidation and antioxidant protection. Results. The development of insulin resistance and obesity in a diet loaded with fructose and fats is accompanied by the development of endothelial dysfunction: in the blood serum, the level of endothelin-1 increases and inducible NO-synthase (iNO-synthase) is activated, in the myocardium, the activity of iNO-synthase increases compared to the data in animals who received a standard diet. The development of oxidative stress in experimental animals characterizes an increase in the content of diene conjugates and thiobarbituric acid-reactive substances in blood serum and myocardium against the background of inhibition of serum antioxidant enzymes (catalase, superoxide dismutase, ceruloplasmin, glutathione peroxidase, glutathione reductase). The degree of endothelial dysfunction and the intensity of lipoperoxidation increase with hypothyroid dysfunction against the background of iodine deficiency. Conclusions. Metabolic disorders under the conditions of insulin resistance and obesity are characterized by the development of endothelial dysfunction and oxidative stress, which are the predictors of the development of cardiovascular risks. Their intensity depends on carbohydrate and thyroid homeostasis.

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State of endothelial function, lipid spectrum and features of coronary vessels structure of rats with obesity and insulin resistance under iodine deficiency conditions

Cited by 2 publications

References 12 publications

Predictors of Hepatocytes Cytolysis of Insulin Resistant Rats Under Conditions of Pre- And Postnatal Iodine Deficiency

Predictors of Hepatocytes Cytolysis of Insulin Resistant Rats Under Conditions of Pre- And Postnatal Iodine Deficiency

Changes of endothelial function and oxidant status in insulin resistance and obesity under the conditions of iodine deficiency

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