State transitions revisited—a buffering system for dynamic low light acclimation of Arabidopsis

Tikkanen, Mikko; Piippo, Mirva; Suorsa, Marjaana; Sirpiö, Sari; Mulo, Paula; Vainonen, Julia P.; Vener, Alexander V.; Allahverdiyeva, Yagut; Aro, Eva‐Mari

doi:10.1007/s11103-006-9088-9

Cited by 147 publications

(185 citation statements)

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“…The levels of NDH subunits and the Cyt b 6 f subunit were slightly up-regulated at higher light intensity in the wild type (Fig. 5, A and B), which is in line with a previous report (Tikkanen et al, 2006). However, the levels of NDH subunits were significantly reduced in lhca5 lhca6 seedlings exposed to high-light conditions compared with those cultured in low-light conditions (Fig.…”

Section: Accumulation Of Monomeric Ndh Under High-light Conditionssupporting

confidence: 91%

Supercomplex Formation with Photosystem I Is Required for the Stabilization of the Chloroplast NADH Dehydrogenase-Like Complex in Arabidopsis

Peng

Shikanai

2011

Plant Physiology

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In higher plants, the chloroplast NADH dehydrogenase-like complex (NDH) interacts with photosystem I (PSI) to form the NDH-PSI supercomplex via two minor light-harvesting complex I (LHCI) proteins, Lhca5 and Lhca6. Previously, we showed that in lhca5 and lhca6, NDH still associates with PSI to form smaller versions of the NDH-PSI supercomplex, although their molecular masses are far smaller than that of the full-size NDH-PSI supercomplex. In this study, we show that the NDH complex is present in the monomeric form in Arabidopsis (Arabidopsis thaliana) lhca5 lhca6, implying that NDH interacts with multiple copies of PSI. NDH subunit levels were slightly reduced in immature leaves and more drastically (approximately 50%) in mature leaves of the lhca5 lhca6 double mutant compared with the wild type. Chlorophyll fluorescence analyses detected NDH activity of lhca5 lhca6, suggesting that the supercomplex formation is not essential for NDH activity. However, the severe phenotypes of the lhca5 lhca6 proton gradient regulation5 triple mutant in both plant growth rate and photosynthesis suggest that the function of NDH was impaired in this mutant in vivo. Accumulation of NDH subunits was drastically reduced in lhca5 lhca6 when the light intensity was shifted from 50 to 500 mmol photons m 22 s 21 . Furthermore, the half-life of NDH subunits, especially that of NDH18, was shorter in monomeric NDH than in the NDH-PSI supercomplex under the high-light conditions. We propose that NDH-PSI supercomplex formation stabilizes NDH and that the process is especially required under stress conditions.

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Section: Accumulation Of Monomeric Ndh Under High-light Conditionssupporting

confidence: 91%

Supercomplex Formation with Photosystem I Is Required for the Stabilization of the Chloroplast NADH Dehydrogenase-Like Complex in Arabidopsis

Peng

Shikanai

2011

Plant Physiology

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“…Analysis of the chlorophyll a/b ratio and F s /F m parameters indeed indicated that the stn7 mutant lacks not only state transitions but also the LTR (Bonardi et al, 2005;Tikkanen et al, 2006). Thus, it appears that the STN7 kinase represents a common redox sensor and/or signal transducer for both responses, supporting earlier suggestions that the two processes are subject to regulatory coupling (Allen, 1995;Allen and Pfannschmidt, 2000;Pursiheimo et al, 2001).…”

Section: Introductionsupporting

confidence: 70%

“…Moreover, plant development and fitness are only marginally affected in A. thaliana mutants impaired in state transitions (Lunde et al, 2000;Bonardi et al, 2005;Tikkanen et al, 2006), even under fluctuating light (Bellafiore et al, 2005) or field conditions (Frenkel et al, 2007) when the ability to adapt to illumination changes should become crucial. Therefore, it has been speculated that state transitions play a less prominent role in land plants than in green algae (Finazzi et al, 2002;Finazzi, 2005), becoming important only under low light conditions (Tikkanen et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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ArabidopsisSTN7 Kinase Provides a Link between Short- and Long-Term Photosynthetic Acclimation

et al. 2009

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Flowering plants control energy allocation to their photosystems in response to light quality changes. This includes the phosphorylation and migration of light-harvesting complex II (LHCII) proteins (state transitions or short-term response) as well as long-term alterations in thylakoid composition (long-term response or LTR). Both responses require the thylakoid protein kinase STN7. Here, we show that the signaling pathways triggering state transitions and LTR diverge at, or immediately downstream from, STN7. Both responses require STN7 activity that can be regulated according to the plastoquinone pool redox state. However, LTR signaling does not involve LHCII phosphorylation or any other state transition step. State transitions appear to play a prominent role in flowering plants, and the ability to perform state transitions becomes critical for photosynthesis in Arabidopsis thaliana mutants that are impaired in thylakoid electron transport but retain a functional LTR. Our data imply that STN7-dependent phosphorylation of an as yet unknown thylakoid protein triggers LTR signaling events, whereby an involvement of the TSP9 protein in the signaling pathway could be excluded. The LTR signaling events then ultimately regulate in chloroplasts the expression of photosynthesis-related genes on the transcript level, whereas expression of nuclear-encoded proteins is regulated at multiple levels, as indicated by transcript and protein profiling in LTR mutants.

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“…For example, by subjecting Arabidopsis plants to alternative periods of low light and high light (HL), LHCII is phosphorylated during the low light and dephosphorylated during the HL periods [17]. The loss of STN7 in plants subjected to this fluctuating light regime leads to a severe decrease in growth, indicating that STN7 has an important role in response to environmental changes [5,18]. The loss of STN8 resulted in slower growth in rice [19], but not in Arabidopsis [9].…”

Section: Introductionmentioning

confidence: 99%

Natural variation in phosphorylation of photosystem II proteins in Arabidopsis thaliana : is it caused by genetic variation in the STN kinases?

Flood

Yin

Herdean

et al. 2014

Phil. Trans. R. Soc. B

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Reversible phosphorylation of photosystem II (PSII) proteins is an important regulatory mechanism that can protect plants from changes in ambient light intensity and quality. We hypothesized that there is natural variation in this process in Arabidopsis ( Arabidopsis thaliana ), and that this results from genetic variation in the STN7 and STN8 kinase genes. To test this, Arabidopsis accessions of diverse geographical origins were exposed to two light regimes, and the levels of phospho-D1 and phospho-light harvesting complex II (LHCII) proteins were quantified by western blotting with anti-phosphothreonine antibodies. Accessions were classified as having high, moderate or low phosphorylation relative to Col-0. This variation could not be explained by the abundance of the substrates in thylakoid membranes. In genotypes with atrazine-resistant forms of the D1 protein, low D1 and LHCII protein phosphorylation was observed, which may be due to low PSII efficiency, resulting in reduced activation of the STN kinases. In the remaining genotypes, phospho-D1 levels correlated with STN8 protein abundance in high-light conditions. In growth light, D1 and LHCII phosphorylation correlated with longitude and in the case of LHCII phosphorylation also with temperature variability. This suggests a possible role of natural variation in PSII protein phosphorylation in the adaptation of Arabidopsis to diverse environments.

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State transitions revisited—a buffering system for dynamic low light acclimation of Arabidopsis

Abstract: Due to an unfortunate misunderstanding, the given names and surnames of all authors have been interchanged in the official publication. The correct authors' names are listed above and below.

Cited by 147 publications

References 0 publications

Supercomplex Formation with Photosystem I Is Required for the Stabilization of the Chloroplast NADH Dehydrogenase-Like Complex in Arabidopsis

Supercomplex Formation with Photosystem I Is Required for the Stabilization of the Chloroplast NADH Dehydrogenase-Like Complex in Arabidopsis

ArabidopsisSTN7 Kinase Provides a Link between Short- and Long-Term Photosynthetic Acclimation

Natural variation in phosphorylation of photosystem II proteins in Arabidopsis thaliana : is it caused by genetic variation in the STN kinases?

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