Static decolorization of polysaccharides from the leaves of Rhododendron dauricum: Process optimization, characterization and antioxidant activities

Hu, Zhengyu; Yu, Ruiying; Sun, Jinfeng; Duan, Yuanqi; Zhou, Hongli; Zhou, Wei; Li, Gao

doi:10.1016/j.procbio.2022.06.025

Cited by 13 publications

(15 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As shown in Figure 4 , AcRDP-1 and AcRDP-2 were composed of Man, Glc, Gal, and Ara with molar ratios of 1.00:5.01:1.17:0.15 and 1.00:4.47:2.39:0.88, respectively. The results showed that the monosaccharide types of the two acetylated polysaccharides were the same as those of the unmodified polysaccharides in the previous study [ 3 ], and they consisted of four monosaccharides. However, the molar ratio of each monosaccharide of acetylated polysaccharides was changed.…”

Section: Resultssupporting

confidence: 67%

“…The signal peak at about 1070 cm −1 was ascribed to the symmetric stretching vibration of the C-O-C [ 32 ]. Compared to the unmodified polysaccharides that were reported in our previous study [ 3 ], the new absorption peak that was found at about 1740 cm −1 was attributed to the vibration of C=O of the O-acetyl group, and the enhanced absorption peak at around 1245 cm −1 was caused by the asymmetric stretching vibration of C-O-C in carbonyl groups, which proved that the two acetylated polysaccharides were successfully modified [ 6 , 33 ].…”

Section: Resultsmentioning

confidence: 54%

“…The polysaccharides from R. dauricum leaves (RDPs) were extracted and isolated according to the description of our previous work [ 3 ]. Briefly, the dried R. dauricum leaves were extracted with distilled water (30:1 mL/g) at 85 °C for 1.5 h. Then, the crude RDPs were distilled with water, and decolorized with D101 macroporous adsorption resin, and deproteinized with the Sevag solvent.…”

Section: Methodsmentioning

confidence: 99%

“…dauricum are of high economic value as a medical herb, containing various chemical constituents (e.g., volatile oil, polysaccharides, flavonoids), and is commonly applied for the treatment of pulmonary infectious diseases (e.g., cough, asthma, acute, and chronic bronchitis) [ 2 ]. Polysaccharides as the main bioactive component of R. dauricum leaves , our previous study showed that the two neutral polysaccharides that were derived from R. dauricum leaves (RDP-1: 1.0948 × 10 4 kDa and RDP-2: 1.1899 × 10 5 kDa) were composed of mannose, glucose, galactose, and arabinose [ 3 ]. However, the antioxidant activity of RDP-1 and RDP-2 is weak, which is unsatisfactory for the utilization of polysaccharides from R. dauricum leaves (RDPs).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Acetylation Modification, Characterization, and Anticomplementary Activity of Polysaccharides from Rhododendron dauricum Leaves

Sun

Jin

et al. 2022

Polymers

Self Cite

View full text Add to dashboard Cite

This study focuses on the acetylation modification of polysaccharides from Rhododendron dauricum leaves (RDPs) with a high degree of substitution (DS) and then discusses their characterization and biological activity. The optimum acetylation conditions of RDPs were optimized by response surface methodology, which were reaction time 3 h, reaction temperature 50 °C, and the liquid-solid ratio 16 mL/g. Under the optima schemes, two eco-friendly acetylated polysaccharides from R. dauricum leaves (AcRDP-1 with DS of 0.439 ± 0.025 and AcRDP-2 with DS of 0.445 ± 0.022) were prepared. The results of structural characterization showed that the AcRDP-1 (9.3525 × 103 kDa) and AcRDP-2 (4.7016 × 103 kDa) were composed of mannose, glucose, galactose, and arabinose with molar ratios of 1.00:5.01:1.17:0.15 and 1.00:4.47:2.39:0.88, respectively. Compared with unmodified polysaccharides, the arabinose content and molecular weight of the two acetylated polysaccharides decreased, and their triple helix conformation disappeared, and further improved their anticomplementary activity. The two acetylated polysaccharides showed stronger a complement inhibition effect than the positive drug by blocking C2, C3, C4, C5, C9, and factor B targets in the classical and alternative pathways. This research indicated that acetylation modification could effectively enhance the anticomplementary activity of RDPs, which is beneficial for the development and utilization of R. dauricum leaves.

show abstract

Section: Resultssupporting

confidence: 67%

Section: Resultsmentioning

confidence: 54%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Acetylation Modification, Characterization, and Anticomplementary Activity of Polysaccharides from Rhododendron dauricum Leaves

Sun

Jin

et al. 2022

Polymers

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition, the positive effect of the galactose content in polysaccharides on the AOA was reported [ 4 ], which is consistent with the data on the monosaccharide composition: the highest galactose content is observed in sample 100-4. According to [ 48 , 49 ], the polysaccharides with a relatively low molecular weight show a stronger ability to absorb DPPH radicals. It is important to note, however, that, although sample 70-4 has the highest molecular weight (33,142 g/mol), its AOA is similar to that of sample 100-4.…”

Section: Resultsmentioning

confidence: 99%

Composition and Structure of Aspen (Pópulus trémula) Hemicelluloses Obtained by Oxidative Delignification

et al. 2022

View full text Add to dashboard Cite

In this study, hemicelluloses of aspen wood (Pópulus trémula) were obtained by oxidative delignification in an acetic acid‒water‒hydrogen peroxide medium at temperatures of 70‒100 °C and a process time of 1‒4 h. The maximum polysaccharide yield of up to 9.68 wt% was reported. The composition and structure of the hemicelluloses were studied using a complex of physicochemical methods: gas and gel permeation chromatography, Fourier-transform infrared spectroscopy, 2D nuclear magnetic resonance spectroscopy, and thermogravimetric analysis. The xylose, mannose, galactose, and glucose monomer units were identified in the hemicelluloses by gas chromatography. The weight average molecular weight Mw of the products determined by gel permeation chromatography was found to range within 8932‒33,142 g/mol. The reported Fourier-transform spectra of the hemicelluloses contain all the bands characteristic of heteropolysaccharides; a weak lignin absorption signal in the spectra at 1500‒1510 cm−1 is attributed to a minor content of phenolic fragments in the structure of the obtained hemicelluloses. The use of thermogravimetric analysis established that the hemicelluloses isolated from aspen wood are resistant against heating to temperatures of up to 90–100 °C and, upon further heating up to 400 °C, start destructing at an increasing rate. The antioxidant activity of the hemicelluloses was examined using the compounds that mimic free radicals (1,1-diphenyl-2-picrylhydrazyl) and hydroxyl radicals (salicylic acid). It was found that the activity of all polysaccharides in neutralizing DPPH and hydroxyl radicals is lower than the absorption capacity of vitamin C at all the tested concentrations (0.5, 2, and 5 mg/mL) and attains 81.7 and 82.9%, respectively.

show abstract

Guide for application of macroporous adsorption resins in polysaccharides purification

Zhou,

Dai,

Zhang

et al. 2024

eFood

View full text Add to dashboard Cite

As a highly selective, recoverable, and low‐cost process, macroporous resins (MPRs) are of interest for the purification of bioactive components from natural products. This guide takes super‐crosslinked MPR as an example, and focuses on application of macroporous adsorption resin in polysaccharide purification, including pretreatment and regeneration of macroporous adsorption resin, various factors affecting the adsorption effect of macroporous adsorption resin, and adsorption mechanism of resin.

show abstract

Static decolorization of polysaccharides from the leaves of Rhododendron dauricum: Process optimization, characterization and antioxidant activities

Cited by 13 publications

References 47 publications

Acetylation Modification, Characterization, and Anticomplementary Activity of Polysaccharides from Rhododendron dauricum Leaves

Acetylation Modification, Characterization, and Anticomplementary Activity of Polysaccharides from Rhododendron dauricum Leaves

Composition and Structure of Aspen (Pópulus trémula) Hemicelluloses Obtained by Oxidative Delignification

Guide for application of macroporous adsorption resins in polysaccharides purification

Contact Info

Product

Resources

About