Static Headspace GC-MS Analysis for Volatile Components in Eleocharis Tuberosa Peel

Abstract

Abstract. Volatile components of Eleocharis tuberosa peels were extracted with absolute ether by ultrasonic wave assisted evaporating. The experiment mainly researches on three positive factors solid-liquid ratio, extraction time and ultrasonic power influencing the extraction efficiency. According to the results of one factor optimization, the orthogonal experiment L 9 (3 4 ) was designed to confirm the optimum extraction conditions. The volatile chemical constituents of the extract were separated and identified by GC-MS. The optimal extraction conditions were as follows: solid-liquid ratio was 1:25 g/mL, extraction time was 60 min and ultrasonic power was 250 w. Confirmed by the practice, the average extraction rate was 3.25%, the conditions shown that the method was credible and reliable. There were 10 volatile compositions in the extract from E. tuberosa peels by the ultrasonic assisted extraction method. The relative content of volatile compositions were 2,6-Di-tert-butyl-4-methoxyphenol (45.24%), Ageratochromene II (23.17%), 3,5-Bis(1,1-dimethylethyl) phenol (9.18%), 9,12-Octadecadienoic acid ethyl ester (5.84%), Ethyl palmitate (4.94%), 1-Linolenoylglycerol (4.11%), Dimethyl phthalate (3.30%), 1,3-Dimethyl-5-tert-butyl-2-acetophenone (2.10%), Phenylacetaldehyde (1.42%), Cedrol (0.70%).