Static Magnetic Fields Effects on Polysaccharides Production by Different Microalgae Strains

BACKGROUND: Tea polysaccharide conjugate (TPC) is a naturally occurring active substance that is extracted from tea. Owing to its benefits in enhancing human immunity and antioxidant effects, TPC is widely used in culinary products. The binding mode of polysaccharides and proteins in TPC, however, has not been well studied; it may be closely related to their functional properties, especially emulsification. RESULTS:The molecular weights and monosaccharide compositions of TPC were determined by ion chromatography and highperformance gel permeation chromatography. Although the functional groups of polysaccharides and proteins were confirmed by infrared spectroscopy, the presence of proteins could not be detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis and ultraviolet spectroscopy. It was hypothesized that the hydrophobic groups of the proteins in TPC were wrapped by polysaccharide chains, thus making the proteins undetectable. The rheology and interfacial protein adsorption results show that TPC forms a viscoelastic film at the oil-water interface to prevent the aggregation of oil droplets, thereby enhancing the stability of the emulsion. Based on these structural and emulsifying properties of TPC, the binding mode of polysaccharides and proteins along with their phase behavior at the oil-water interface of the emulsion was speculated.CONCLUSION: In TPC, the hydrophilic groups of the proteins are linked to polysaccharides by covalent interactions, where the hydrophobic groups are wrapped with the polysaccharide chains with the help of hydrophobic forces to form a hydrophobic core. The unique binding of polysaccharides and proteins in TPC enhances its amphiphilic properties, which can be effectively distributed at the oil-water interface and form stable emulsions.

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“…Glyoxylate of TPC was evaluated with 3‐phenylphenol, as described by Deamici et al ., using galactose aldehyde acid as standard 10 …”

Section: Methodsmentioning

confidence: 99%

Structural properties of Phoenix oolong tea polysaccharide conjugates and the interfacial stability in nanoemulsions

Lin

Zhu

et al. 2023

J Sci Food Agric

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“…Finally, in a recent study, [ 182 ] have studied the effect of a static magnetic field on Limnospira indica PCC8005 (formerly known as Arthrospira indica PCC8005) EPS production and composition. If static magnetic fields have an impact on the growth rate, pigment content, and intracellular polysaccharide accumulation, it seems that the EPS production yield is not increased.…”

Section: Culture Strategies For Eps Production and Impact On Compositionmentioning

confidence: 99%

Exopolysaccharides from Microalgae and Cyanobacteria: Diversity of Strains, Production Strategies, and Applications

Laroche

2022

Marine Drugs

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Microalgae and cyanobacteria are photosynthetic organisms that can produce/accumulate biomolecules with industrial interest. Among these molecules, EPSs are macromolecular polysaccharidic compounds that present biological activities and physico-chemical properties, allowing to consider their valorization in diverse commercial markets, such as cosmetic, therapeutic, nutraceutic, or hydrocolloids areas. The number of microalgae and cyanobacteria strains described to produce such EPSs has increased in recent years as, among the 256 producing strains gathered in this review, 86 were published in the last 10 years (~33%). Moreover, with the rise of research on microalgae EPSs, a variety of monosaccharides compositions have been discovered, highlighting the versatility of these organisms. If some production strategies can be applied to increase EPS production yields, it appears that case by case studies are needed to promote EPS synthesis by a strain, as many responses exist. This paper proposes an up-to-date state of the art of the diversity of microalgae and cyanobacteria EPS-producing strains, associated to the variability of compositions. The strategies for the production and extraction of the polymers are also discussed. Finally, an overview of the biological activities and physico-chemical properties allow one to consider their use on several commercial markets.

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“…Monosaccharide compositions of polysaccharides were evaluated by High Pressure Anion Exchange Chromatography (HPAEC) on an ICS 3000 (Dionex, Sunnyvale, CA, USA) equipped with pulsed amperometric detection (PAD) and AS 50 autosampler. as previously described [6,9,27,87]. It was assembled with a guard CarboPac PA1-column (4 × 50 mm) and analytical CarboPac PA1-column (4 × 250 mm).…”

Section: Monosaccharides Compositionmentioning

confidence: 99%

Exploring the Diversity of Red Microalgae for Exopolysaccharide Production

2022

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Microalgae constitute a remarkable biological diversity but a limited number of them have been the object of study for their ability to produce exoplysaccharides (EPS). Among them, the red marine microalgae Porphyridium or Rhodella produce sulphated EPS, exhibiting some biological activities with potential interest in the pharmaceutical and cosmetic industries. EPS from Porphyridium and Rhodella being relatively similar in their composition, it has long been considered that all the red microalgae produced similar EPS and no attention was paid to other red microalgae. The objective of our work was then to explore the diversity of red microalgae for the production of EPS, focusing in this first step on the screening of the strains for their ability to produce EPS and preliminary structural characterization. The study was conducted with 11 microalgae strains belonging to the proteorhodophytina subphylum. All microalgae were able to produce EPS, released in the culture medium (strains belonging to Porphyridiophyceae and Rhodellophyceae classes) or remaining bound to the cells (strains from Stylonematophyceae class). The analysis of monosaccharides composition was found significantly different, with for instance high levels of glucuronic acids in the EPS from C. japonica and N. cyanea, but also strong differences in the sulphation degrees of polymers (between 1.2 and 28.7% eq. SO4).

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Static Magnetic Fields Effects on Polysaccharides Production by Different Microalgae Strains

Cited by 24 publications

References 61 publications

Structural properties of Phoenix oolong tea polysaccharide conjugates and the interfacial stability in nanoemulsions

Structural properties of Phoenix oolong tea polysaccharide conjugates and the interfacial stability in nanoemulsions

Exopolysaccharides from Microalgae and Cyanobacteria: Diversity of Strains, Production Strategies, and Applications

Exploring the Diversity of Red Microalgae for Exopolysaccharide Production

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