Stationary Phases for Packed Column Supercritical Fluid Chromatography

Insights into chiral recognition mechanisms in supercritical fluid chromatography V. Effect of the nature and proportion of alcohol mobile phase modifier with amylose and cellulose tris-(3,5-dimethylphenylcarbamate) stationary phases

“…Many papers have been published on the SFC separation of enantiomers using CO 2 mixed with alcohol solvents [12,19,21]. Alcohol modifiers affect the quality of the enantioseparation in numerous ways and the changes are difficult to predict.…”

Theory and Practice of UHPLC and UHPLC–MS

“…To prompt the elution and shorten the analysis time of polar and/or ionizable compounds, the addition of a small amount of a more polar organic modifier (cosolvent) is often needed (Tarafder, 2016;. The role of the organic modifier in SFC involves several functions (Tarafder, 2016;Poole, 2012;Berger, 1997;Strubinger et al, 1991):…”

“…For the conditions in pSFC, significant amounts of both CO 2 and an organic modifier are adsorbed by silica and chemically bonded stationary phases and function as components of the stationary phase (Poole, 2012). Typical organic modifiers most commonly employed in SFC include not only alcohols, namely methanol, ethanol, and isopropanol but also other solvents, such as acetonitrile or some less polar solvents may be used.…”

“…The concentration of additives in the mobile phase depends on the nature of the analytes and stationary phase and should be optimized during method development. Although the mode of action is not always clearly elucidated for additives, they are supposed to act via several different mechanisms, as was discussed in detail in recently published papers (Tarafder, 2016;Poole, 2012;Wen and Olesik, 2000):…”

Evaluation of an amide‐based stationary phase for supercritical fluid chromatography

“…A historical perspective on the development of SFC, the current state of the art, and how the technique has gained popularity are readily available in the current literature . Today, SFC is mostly practiced using CO 2 as the mobile phase with methanol, or other alcohols , as cosolvent modifiers that can be adjusted during the separation by gradient elution, if necessary, to increase mobile phase elution strength .…”