Statistical Anatomy of Microvascular Networks in Living Mouse Cortex

Mihelic, Samuel A.; Engelmann, Shaun; Zhou, Annie; Jafari, Chakameh Z.; Sullender, Colin T.; Santorelli, Adam; Dunn, Andrew K.

doi:10.1364/translational.2022.jm3a.57

Cited by 1 publication

(1 citation statement)

References 2 publications

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“…In future work, we hope to address this limitation by making use of specific variants of ICG [37] which have higher clearance time, enabling 3D vascular analysis. A three-dimensional structure of the brain is important to understand the structure of the brain in healthy [38, 39] and diseased mice [40].…”

Section: Discussionmentioning

confidence: 99%

Non-Degenerate Two-Photon Imaging of Deep Rodent Cortex using Indocyanine Green in the water absorption window

Tomar,

Engelmann,

Woods

et al. 2024

Preprint

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We present a novel approach for deep vascular imaging in rodent cortex at excitation wavelengths susceptible to water absorption using two-photon microscopy with photons of dissimilar wavelengths. We demonstrate that non-degenerate two-photon excitation (ND-2PE) enables imaging in the water absorption window from 1400-1550 nm using two synchronized excitation sources at 1300 nm and 1600 nm that straddle the absorption window. We explore the brightness spectra of indocyanine green (ICG) and assess its suitability for imaging in the water absorption window. Further, we demonstrate in vivo imaging of the rodent cortex vascular structure up to 1.2 mm using ND-2PE. Lastly, a comparative analysis of ND-2PE at 1435 nm and single-wavelength, two-photon imaging at 1300 nm and 1435 nm is presented. Our work extends the excitation range for fluorescent dyes to include water absorption regimes and underscores the feasibility of deep two-photon imaging at these wavelengths.

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