Statistical modelling of eugenol benzoate synthesis using Rhizomucor miehei lipase reinforced nanobioconjugates

Manan, Fatin Myra Abd; Rahman, Ida Nurhazwani Abd; Marzuki, Nur Haziqah Che; Mahat, Naji Arafat; Huyop, Fahrul; Wahab, Roswanira Abdul

doi:10.1016/j.procbio.2015.12.002

Cited by 40 publications

(28 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Effect Of Reaction Parametersmentioning

confidence: 99%

“…In the chart, it is possible to observe positive and negative values; this implies that the independent variables that present influence under the response in a positive way, the higher the value, the higher the percentage in esters conversion. And for the variables that present a negative influence, the higher the negative value, the lower the percentage in esters conversion [74,75]. The interaction between the independent variables and the dependent variable is shown by the three-dimensional response of the fatty acid ethyl esters (FAEE) yield in Figure 3, in which several response surfaces are created for the relationship between the effects of two parameters on the FAEE yield while the third invariable.…”

Section: Effect Of Reaction Parametersmentioning

confidence: 99%

See 1 more Smart Citation

Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil (Orbignya sp.) via RSM

et al. 2020

View full text Add to dashboard Cite

Residual oil from babassu (Orbignya sp.), a low-cost raw material, was used in the enzymatic esterification for biodiesel production, using lipase B from Candida antarctica (Novozym® 435) and ethanol. For the first time in the literature, residual babassu oil and Novozym® 435 are being investigated to obtain biodiesel. In this communication, response surface methodology (RSM) and a central composite design (CCD) were used to optimize the esterification and study the effects of four factors (molar ratio (1:1–1:16, free fatty acids (FFAs) /alcohol), temperature (30–50 °C), biocatalyst content (0.05–0.15 g) and reaction time (2–6 h)) in the conversion into fatty acid ethyl esters. Under optimized conditions (1:18 molar ratio (FFAs/alcohol), 0.14 g of Novozym® 435, 48 °C and 4 h), the conversion into ethyl esters was 96.8%. It was found that after 10 consecutive cycles of esterification under optimal conditions, Novozym® 435 showed a maximum loss of activity of 5.8%, suggesting a very small change in the support/enzyme ratio proved by Fourier Transform Infrared (FTIR) spectroscopy and insignificant changes in the surface of Novozym® 435 proved by scanning electron microscopy (SEM) after the 10 consecutive cycles of esterification.

show abstract

Section: Effect Of Reaction Parametersmentioning

confidence: 99%

Section: Effect Of Reaction Parametersmentioning

confidence: 99%

Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil (Orbignya sp.) via RSM

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Like any other formulations used in detergents or in enzyme-assisted manufacturing processes (WAHAB et al, 2012a;WAHAB et al, 2014;MOHAMAD et al, 2015a;MANAN et al,. 2016), the constituents proposed in our enzymebased formulation is considerably less toxic than that found in chemical fungicides.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, manufacturing processes that use natural enzymes as catalysts are typically carried out under near ambient conditions i.e. (1 atm, 30-50 o C, pH 6-8) (KARBOUNE et al, 2008;MARZUKI et al, 2015a;MARZUKI et al, 2015b;MOHAMAD et al, 2015b;MARZUKI et al, 2015c;MANAN et al, 2016;KHALILI et al, 2017), similar to conditions under agricultural settings. For these reasons, an enzyme-based fungicide i.e.…”

Section: Introductionmentioning

confidence: 99%

Assessments on the catalytic and kinetic properties of beta-glucosidase isolated from a highly efficient antagonistic fungus Trichoderma harzianum

Khalili¹,

Huyop²,

Javed³

et al. 2018

Biosci. J.

Self Cite

View full text Add to dashboard Cite

Due to the toxicity and inefficiency of chemical fungicides to control infestation of Macrophomina phaseolina (Tassi) Goid which causes charcoal rot in plants, a biotechnological approach using βglucosidase (EC.3.2.1) as the alternative bioactive ingredient in fungicide is hereby, proposed. The extracellular enzyme was isolated from a highly efficient fungal antagonist, Trichoderma harzianum T12. The highly similar molecular masses obtained using SDS-PAGE (96 kDa) and MALDI-TOF mass spectrometry (98.3 kDa) affirmed that the β-glucosidase was purified to homogeneity. Consequently, optimum catalytic parameters that rendered the highest enzyme activity were found to be: 45˚C, pH 7, inoculum size of 10 % (w/v), supplementation with metal ions Zn 2+ and Mn 2+ ions, and Tween 80. Addition of wheat bran and (NH 4 ) 2 SO 4 as carbon and nitrogen sources also improved enzyme activity. BLASTn showed the sequence of β-glucosidase T12 was highly identical to other β-glucosidases viz. T. harzianum strain IOC-3844 (99%), T. gamsii and T. virens bgl1 (86 %) as well as T. reesei strain SJVTR and T. viride strain AS 3.3711 (84 %). Kinetic assessment showed that β-glucosidase T12 catalyzes hydrolytic activity is characterized by a K m of 0.79 mM and V max of 8.45 mM min -1 mg -1 protein, with a corresponding k cat of 10.69 s -1 .

show abstract

“…Another crucial aspect is the optimization of the fungal cultivation media to produce b-glucosidase. This is because the conventional approach of optimization processes is laborious due to screening processes that involve a large number of variables [16,17]. Moreover, the approach is incapable of detecting the true optimum [18] following its one-factor-at-a-time design, which is unable to explicitly illustrate the complete interactive effects of various factors on the response [19].…”

Section: Introductionmentioning

confidence: 99%

Optimization of cultivation conditions in banana wastes for production of extracellular β-glucosidase byTrichoderma harzianumRifai efficient forin vitroinhibition ofMacrophomina phaseolina

Khalili

Huyop

Manan

et al. 2017

Biotechnology & Biotechnological Equipment

Self Cite

View full text Add to dashboard Cite

The present study was carried out to optimize the physical and nutritional parameters for cultivation of Trichoderma harzianum Rifai for production of extracellular b-glucosidase, an alternative to chemcial fungicides to control Macrophomina phaseolina, which causes charcoal rot disease in crops. Response surface methodology by the Box-Behnken design (BBD) based on four factors (temperature, carbon sources, inoculum size and pH) was used for optimization of the cultivation conditions to produce b-glucosidase using agro-industrial banana wastes. The highest b-glucosidase activity (1483.27 U/mL) was attained under optimized cultivation conditions: »36 C and pH 7.3, using carbon source and inoculum size of 10% (w/v) and 5% (w/v), respectively. It is noteworthy that the low deviation values (0.09¡0.44%) in the verification experiments (R1, R2 and R3) inferred the generated model was accurate to predict optimal cultivation conditions of T. harzianum Rifai. Likewise, the obtained diameters of inhibition zones ranging from 58.00¡38.66 mm following treatment with b-glucosidase was found comparable to other in vitro tests using pure T. harzianum isolates and chemical fungicides. Hence, the findings indicated that it was feasible to use b-glucosidase as a greener alternative to chemical fungicides for control of M. phaseolina infection and consequently, for protection of crops against charcoal rot disease.

show abstract

Statistical modelling of eugenol benzoate synthesis using Rhizomucor miehei lipase reinforced nanobioconjugates

Cited by 40 publications

References 55 publications

Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil (Orbignya sp.) via RSM

Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil (Orbignya sp.) via RSM

Assessments on the catalytic and kinetic properties of beta-glucosidase isolated from a highly efficient antagonistic fungus Trichoderma harzianum

Optimization of cultivation conditions in banana wastes for production of extracellular β-glucosidase byTrichoderma harzianumRifai efficient forin vitroinhibition ofMacrophomina phaseolina

Contact Info

Product

Resources

About