Statistical optimization of a novel extracellular alkaline and thermostable amylase production from thermophilic Actinomadura keratinilytica sp. Cpt29 and its potential application in detergent industry

Kherouf, Marwa; Habbeche, Amina; Benamia, Fatiha; Saoudi, Boudjema; Kerouaz, Bilal; Ladjama, Ali

doi:10.1016/j.bcab.2021.102068

Cited by 8 publications

(3 citation statements)

References 48 publications

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“…The obtained data revealed that the optimum conditions for amylase production by Rhizopus stolonifer were 30 • C, a pH value of 6, and an incubation time of 8 days, using yeast extract (3 g/L) and an inoculum size of 2 mL/25 mL. Vidyalakshmi et al [28] stated that incubation temperature is considered one of the significant parameters which considerably influences amylase production [29]. Interestingly, many studies on amylase production using different fungal isolates were performed with mesophiles that grow best at temperatures between 25 and 37 • C [30].…”

Section: Enhancement Of Amylase Enzyme Production On Potato Peel Wastesmentioning

confidence: 99%

Enzymatic-Based Hydrolysis of Digested Potato Peel Wastes by Amylase Producing Fungi to Improve Biogas Generation

et al. 2023

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Potato peel wastes are generated in high quantities from potato processing industries. They are pollutants to the environment, and they release greenhouse gases into the atmosphere. The present study assessed the potentiality of hydrolyzing potato wastes by amylase-producing fungi to improve biogas generation from potato peels through the anaerobic digestion process. Different fungal isolates were screened for amylase production on potato wastes, and the highest amylase producer was selected for optimizing the efficacy of producing amylases in high quantities to efficiently allow the conversion of potato organic matter into fermentable sugars that are utilized for the anaerobic digestion process. The best amylase producers were those derived from Rhizopus stolonifer (32.61 ± 0.89 U/mL). The highest cumulative methane yield from hydrolyzed potato peel was 65.23 ± 3.9 mL CH4/g and the methane production rate was 0.39 mL CH4/h, whereas the highest biogas yield from unhydrolyzed potato wastes was 41.32 ± 2.15 mL CH4/g and the biogas production rate was 0.25 mL CH4/h. Furthermore, it was found that the two combined sequential stages of anaerobic digestion (biogas production) followed by biodiesel production (enzymatic esterification) were the most effective, recording 72.36 ± 1.85 mL CH4/g and 64.82% biodiesel of the total analytes. However, one-pot fermentation revealed that biogas yield was 22.83 ± 2.8 mL CH4/g and the biodiesel extracted was 23.67% of the total analytes. The insights of the current paper may increase the feasibility of potato peel-based biorefinery through the biological hydrolysis strategy of potato wastes using eco-friendly enzymes.

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Section: Enhancement Of Amylase Enzyme Production On Potato Peel Wastesmentioning

confidence: 99%

Enzymatic-Based Hydrolysis of Digested Potato Peel Wastes by Amylase Producing Fungi to Improve Biogas Generation

et al. 2023

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“…Several reports exist of successful RSM application, with methodologies like the Box-Behnken design, Plackett-Burhman factorial design, and central composite design (CCD), for process parameter optimization and attainment of enhanced enzyme production ([ [34] , [39] , [40] , [41] ]; Paul et al., 2021). RSM has been applied successfully for enhanced alpha-amylase production [ [4] , [42] , [43] , [44] , [45] , [46] ].…”

Section: Introductionmentioning

confidence: 99%

Detergent-stable amylase production by Paenibacillus lactis strain OPSA3 isolated from soil; optimization by response surface methodology

Ugwuoji

Nwagu

Ezeogu

2023

Biotechnology Reports

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“…These enzymes catalyze the reactions at low temperatures, reduce the release of harmful components to the environment, and enhance the ability to removing the harsh stains and improve the cloth softness, whiteness, and color (Al-ghanayem, 2020). Numerous examples can be cited from the literature indicating the high potential of alkaline, detergent-compatible α-amylases as an environmentally friendly component in stain removal and brightness of fabric (Hmidet et al, 2019;Allala et al, 2020;Kherouf et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Highly Efficient Computationally Derived Novel Metagenome α-Amylase With Robust Stability Under Extreme Denaturing Conditions

et al. 2021

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α-Amylases are among the very critical enzymes used for different industrial purposes. Most α-amylases cannot accomplish the requirement of industrial conditions and easily lose their activity in harsh environments. In this study, a novel α-amylase named PersiAmy1 has been identified through the multistage in silico screening pipeline from the rumen metagenomic data. The long-term storage of PersiAmy1 in low and high temperatures demonstrated 82.13 and 71.01% activities after 36 days of incubation at 4 and 50°C, respectively. The stable α-amylase retained 61.09% of its activity after 180 min of incubation at 90°C and was highly stable in a broad pH range, showing 60.48 and 86.05% activities at pH 4.0 and pH 9.0 after 180 min of incubation, respectively. Also, the enzyme could resist the high-salinity condition and demonstrated 88.81% activity in the presence of 5 M NaCl. PersiAmy1 showed more than 74% activity in the presence of various metal ions. The addition of the detergents, surfactants, and organic solvents did not affect the α-amylase activity considerably. Substrate spectrum analysis showed that PersiAmy1 could act on a wide array of substrates. PersiAmy1 showed high stability in inhibitors and superb activity in downstream conditions, thus useful in detergent and baking industries. Investigating the applicability in detergent formulation, PersiAmy1 showed more than 69% activity after incubation with commercial detergents at different temperatures (30–50°C) and retained more than 56% activity after incubation with commercial detergents for 3 h at 10°C. Furthermore, the results of the wash performance analysis exhibited a good stain removal at 10°C. The power of PersiAmy1 in the bread industry revealed soft, chewable crumbs with improved volume and porosity compared with control. This study highlights the intense power of robust novel PersiAmy1 as a functional bio-additive in many industrial applications.

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Statistical optimization of a novel extracellular alkaline and thermostable amylase production from thermophilic Actinomadura keratinilytica sp. Cpt29 and its potential application in detergent industry

Cited by 8 publications

References 48 publications

Enzymatic-Based Hydrolysis of Digested Potato Peel Wastes by Amylase Producing Fungi to Improve Biogas Generation

Enzymatic-Based Hydrolysis of Digested Potato Peel Wastes by Amylase Producing Fungi to Improve Biogas Generation

Detergent-stable amylase production by Paenibacillus lactis strain OPSA3 isolated from soil; optimization by response surface methodology

Highly Efficient Computationally Derived Novel Metagenome α-Amylase With Robust Stability Under Extreme Denaturing Conditions

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