Statistical Optimization of Prodigiosin Production by Plackett-burman Design for Bacteria Isolated from Indian Marine Soil

Abstract

The current investigation was conducted to maximise the production of the natural anticancer drug from the microbe isolated from the marine soil sample of the Coromandel Coast of the Bay of Bengal region of India. Yellow to red colour pigmented microbes separated by crowd plate method. Bacteria are producing strong colour product subjected to future study. The isolated strains were detected based on biochemical, morphological, and genetic characteristics. Pigment formation was found to be influenced strongly by conditions of the environment. The water-insoluble pigment extracted by acidified methanol and showed maximum absorbance at 535nm. A statistical screening procedure was adopted to select the optimum condition to produce the pigment. The carbon, nitrogen, medium pH, growth condition temperature and revolution of agitation were screened using the response surface methodology statistical model. The near optimum conditions for the production medium were affected by the concentration of peanut, L-proline, percentage inoculum pH and incubation time. When these conditions were employed yield increased as two-fold as the concentration of prodigiosin 789 mg/l.

An extensive review of marine pigments: sources, biotechnological applications, and sustainability

“… 2019 ). This purification can be achieved by the interaction between enzyme and substrate, antigen and antibody, and receptors and proteins, due to ionic, hydrophobic, hydrogen, and disulfide bond linkage (Kavitha and Damodharan 2021 ; Aman Mohammadi et al. 2022 ).…”

“…5) and can be found in several marine microbes (Darshan and Manonmani 2015). Marine bacteria that synthesize this and related compounds include Serratia, Zooshikella, Streptomyces, Actinomadura, Pseudomonas, Pseudoalteromonas, Hahella, and Vibrio (Zhang et al 2017;Kongkapan et al 2020;Ramesh et al 2020;Setiyono et al 2020;Kavitha and Damodharan 2021;Metwally et al 2021). Serratia rubidaea RAM_Alex isolated from bivalve mollusks, and Zooshikella sp., isolated from the pristine Andaman islands, India, produced prodigiosin that showed antibacterial activity against Staphylococcus aureus and E. coli (Ramesh et al 2020;Metwally et al 2021).…”

“…Affinity chromatography is based on the reversible interaction between the molecules of interest and a specific ligand coupled to a chromatography matrix that presents specific affinity (Praveen et al 2019). This purification can be achieved by the interaction between enzyme and substrate, antigen and antibody, and receptors and proteins, due to ionic, hydrophobic, hydrogen, and disulfide bond linkage (Kavitha and Damodharan 2021;Aman Mohammadi et al 2022). Ni-NTA affinity chromatography and Superdex-200 gel filtration chromatography have been used to purify the thermophilic β-carotene 15,15′-monooxygenase BCMO 7211 isolated from the marine bacterium Candidatus Pelagibacter sp.…”