Status and future of Quantitative Microbiological Risk Assessment in China

Dong, Qingli; Barker, G. C.; Gorris, L.G.M.; Tian, Maoyi; Song, Xiaoyu; Malakar, Pradeep K.

doi:10.1016/j.tifs.2014.12.003

Cited by 41 publications

(23 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The integrated distribution used in this study, which combined two cumulative distributions into a discrete distribution, has been frequently used by Chinese QMRA researchers . Also, a unique advantage of this distribution is that it could simultaneously describe the bacterial contamination in positive and negative samples.…”

Section: Methodscontrasting

confidence: 80%

Prevalence ofVibrio parahaemolyticusin seafood products from hypermarkets in Shanghai

Zhang

Lou

et al. 2016

J. Sci. Food Agric.

View full text Add to dashboard Cite

The low prevalence and burden found indicated that seafood from hypermarkets may not be an important risk source for V. parahaemolyticus infection in Shanghai, and more attention should be paid to other areas for selling seafood, such as farmlands or farmers' markets. The simple and effective lognormal distribution is recommended as a better choice for describing V. parahaemolyticus contamination in future risk assessment studies. © 2016 Society of Chemical Industry.

show abstract

Section: Methodscontrasting

confidence: 80%

Prevalence ofVibrio parahaemolyticusin seafood products from hypermarkets in Shanghai

Zhang

Lou

et al. 2016

J. Sci. Food Agric.

View full text Add to dashboard Cite

show abstract

“…Microbiological hazard analysis and control of fresh seafood products remained a challenge due to microbial contamination and growth (Macé et al 2013). Predictive microbiology model and microbiological risk No significant difference was found in the same behavior parameters between PMA TaqMan-based multiplex real-time PCR and plate counting method (p>0.05) assessment were regarded as important approaches to effectively solve this challenge (Dong et al 2015). The traditional predictive model was based on microbial growth data obtained by culture-dependent methods, where the process was rather time-consuming and labor-intensive (Ye et al 2013;Wang et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Quantifying viable Vibrio parahaemolyticus and Listeria monocytogenes simultaneously in raw shrimp

Zhang

Liu

Lou

et al. 2015

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

A novel TaqMan-based multiplex real-time PCR method combined with propidium monoazide (PMA) treatment was firstly developed for the simultaneous quantification of viable Vibrio parahaemolyticus and Listeria monocytogenes in raw shrimp. The optimization of PMA concentration showed that 100 μM was considered optimal to effectively inhibit 10(8) CFU/mL dead cells of both bacteria. The high specificity of this method was confirmed on tests using 96 target and non-target strains. The optimized assay could detect as low as 10(1)-10(2) CFU/g of each strain on the artificially contaminated shrimp, and its amplification efficiencies were up to 100 and 106 % for V. parahaemolyticus and L. monocytogenes, respectively. Furthermore, this assay has been successfully applied to describe the behavior of these two pathogens in raw shrimps stored at 4 °C. In conclusion, this PMA TaqMan-based multiplex real-time PCR technique, where the whole procedure takes less than 5 h, provides an effective and rapid tool for monitoring contamination of viable V. parahaemolyticus and L. monocytogenes in seafood, improving seafood safety and protecting public health.

show abstract

“…Most Campylobacter infections are associated with consumption of poultry and by‐products that have been contaminated during processing (Hermans and others ; Wagenaar and others ). There are reports to date assessing the risk of Campylobacter in broiler chickens (Dong and others ), and raw poultry meat is considered a high‐risk raw material with regard to the presence of Campylobacter spp. The evisceration stage is the most important step for preventing slaughter contamination, and Campylobacter detection may be employed to indicate microbiological safety (Jacxsens and others ).…”

Section: Characteristics Of Campylobacter Sppmentioning

confidence: 99%

Molecular Detection, Typing, and Quantification of Campylobacter spp. in Foods of Animal Origin

Frasão

Marin

Conté-Júnior

2017

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

Abstract:The most frequently reported zoonosis and the main bacterial foodborne disease infection in humans is caused by Campylobacter spp., and C. jejuni and C. coli are the most common types. These bacteria can be found in the intestinal tracts of cattle, dogs, cats, sheep, poultry and pigs. The isolation of this microorganism is laborious because it requires specific media and a low oxygen concentration for growth. Additionally, differentiation between species through conventional bacteriology is difficult, as there are few different biochemical characteristics among the various species. Molecular microbiological techniques have become more important and are now broadly applied to help overcome difficulties in the identification, differentiation, and quantification of this pathogen. To date, there have been advances in the development and use of molecular techniques for the identification of microorganisms in foodstuffs. Tools such as pulsed-field gel electrophoresis and multilocus sequence typing are the most commonly used for typing. For the identification and confirmation of species, polymerase chain reaction (PCR) is crucial. Quantification by real-time PCR has wide applicability. To identify strains and antimicrobial resistance genes, sequencing technologies have been applied. This review builds on the discussion about the main and most widely used molecular methods for Campylobacter, as well as methods showing better potential for the classification, identification, and quantification of this important pathogen.

show abstract

Status and future of Quantitative Microbiological Risk Assessment in China

Cited by 41 publications

References 42 publications

Prevalence ofVibrio parahaemolyticusin seafood products from hypermarkets in Shanghai

Prevalence ofVibrio parahaemolyticusin seafood products from hypermarkets in Shanghai

Quantifying viable Vibrio parahaemolyticus and Listeria monocytogenes simultaneously in raw shrimp

Molecular Detection, Typing, and Quantification of Campylobacter spp. in Foods of Animal Origin

Contact Info

Product

Resources

About