Steady and pulsatile shear stress induce different three-dimensional endothelial networks through pseudopodium formation

Abe, Yoshinori; Sudo, Ryo; Ikeda, Mariko; Tanishita, Kazuo

doi:10.1007/s12573-012-0056-5

Cited by 2 publications

(5 citation statements)

References 43 publications

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“…In a parallel-plate flow chamber, ECs on a collagen matrix under flow conditions promoted the growth of vascular networks within the matrix compared to static conditions (Ueda et al, 2004). In addition, steady FSS promoted the formation of interconnected and stable vascular networks, while pulsatile FSS induced retraction of vascular networks, resulting in the formation of unstable networks (Abe et al, 2013b). Furthermore, a combination of FSS and angiogenic factors, including S1P, enhanced EC invasion into a collagen matrix via phosphorylation of Akt and matrix metalloproteinase (MMP)-2 activation (Kang et al, 2008).…”

Section: In Vitro Angiogenesis Modelsmentioning

confidence: 99%

Progress and challenges in vascular tissue engineering using self-organization/pre-designed approaches

Watanabe

Sudo

2021

JBSE

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Progress and challenges in vascular tissue engineering using self-organization/pre-designed approaches IntroductionCurrently, organ transplantation is the most effective therapy for end-stage organ failure. However, the demand for life-saving organ transplants far exceeds the supply of available organs, including the kidney, liver, heart, and lung owing to organ shortage (Lauerer et al., 2016). To address this problem, tissue engineering has offered potential strategies for the construction of solid tissues and organs using cells, signaling factors, and scaffolds (Langer and Vacanti, 1993). Over the last several decades, various approaches have been developed for the construction of organ-specific tissues by utilizing cell culture platforms, such as porous scaffolds and decellularized matrices, and scaffold-free culture methods, including cell sheet engineering and spheroid culture (Uygun et al., 2010;Bao et al., 2011;No et al., 2012;Damania et al., 2014). Despite great progress, limitations remain for medical and clinical applications of tissue-engineered constructs owing to the lack of functional vascular networks, which may result in tissue dysfunction, such as hemorrhage, clot formation, and tissue hypoxia.Functional vascular networks should be constructed in tissue-engineered constructs. Establishing a hierarchical tubular network is required to supply oxygen and nutrients to the tissue constructs. In particular, following morphological

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Section: In Vitro Angiogenesis Modelsmentioning

confidence: 99%

Progress and challenges in vascular tissue engineering using self-organization/pre-designed approaches

Watanabe

Sudo

2021

JBSE

Self Cite

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“…Normal sprout development requires a precise balance between migration and proliferation. An imbalance between these two processes may lead to detached tip cells [ 81 ] or to tortuous vessels [ 82 ]. The formation of a lumen is concomitant with sprout elongation, with stalk cells of lumenized sprouts expressing luminal–abluminal polarity [ 83 – 85 ].…”

Section: Early Phases Of Sprouting Angiogenesismentioning

confidence: 99%

“…For instance, unlike steady shear, oscillatory shear stimulates tubulogenesis in venous and microvascular ECs but not in arterial ECs [ 109 ]. Interestingly, pulsatility appears to be insufficient to induce angiogenesis on its own [ 206 ], with pulsatile shear even limiting sprouting compared with steady shear [ 81 ]. Fluctuations may also affect mass transport and autocrine signalling [ 229 ], with effects on angiogenesis that remain unknown.…”

Section: Role Of Fluid Mechanics In Angiogenic Sproutingmentioning

confidence: 99%

“…On the other hand, compared with steady flow, RPF and OF induce Notch upregulation while NPF does not [ 147 ]. However, the overall picture is clouded by results from a three-dimensional network formation study that reported no significant differences in the number of branches and bifurcation points under pulsatile flows of different magnitudes and frequencies [ 81 ].…”

Section: Role Of Fluid Mechanics In Angiogenic Sproutingmentioning

confidence: 99%

“…During the sprout elongation phase, the effects of unsteady shear stress and pressure on the necessary balance between migration and proliferation remain unclear. While 1 Hz non-reversing pulsatile shear appears to maximize EC proliferation [ 239 ], it has been shown to disrupt the migration-proliferation equilibrium, leading to detached tip cells [ 81 ]. The effects of pressure fluctuations on sprout elongation are equally complex, with pulsatile pressures with amplitudes of 40 mm Hg either promoting or impairing EC proliferation, depending on the mean pressure value [ 240 , 241 ].…”

Section: Role Of Fluid Mechanics In Angiogenic Sproutingmentioning

confidence: 99%

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Mechanical regulation of the early stages of angiogenesis

Barrasa-Ramos

Dessalles

Hautefeuille

et al. 2022

J. R. Soc. Interface.

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Favouring or thwarting the development of a vascular network is essential in fields as diverse as oncology, cardiovascular disease or tissue engineering. As a result, understanding and controlling angiogenesis has become a major scientific challenge. Mechanical factors play a fundamental role in angiogenesis and can potentially be exploited for optimizing the architecture of the resulting vascular network. Largely focusing on in vitro systems but also supported by some in vivo evidence, the aim of this Highlight Review is dual. First, we describe the current knowledge with particular focus on the effects of fluid and solid mechanical stimuli on the early stages of the angiogenic process, most notably the destabilization of existing vessels and the initiation and elongation of new vessels. Second, we explore inherent difficulties in the field and propose future perspectives on the use of in vitro and physics-based modelling to overcome these difficulties.

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Steady and pulsatile shear stress induce different three-dimensional endothelial networks through pseudopodium formation

Cited by 2 publications

References 43 publications

Progress and challenges in vascular tissue engineering using self-organization/pre-designed approaches

Progress and challenges in vascular tissue engineering using self-organization/pre-designed approaches

Mechanical regulation of the early stages of angiogenesis

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