Steady state organ distribution and metabolism of thyroxine and 3,5,3'-triiodothyronine in intestines, liver, kidneys, blood, and residual carcass of the rat in vivo.

Abstract

We have directly measured the relative distribution and steady state pool sizes of T4 and T3 in blood and extrathyroidal tissues of the whole euthyroid male rat, plus several steady state T3 and T4 metabolism indices in the whole animal, in eight rats infused sc for 7 days with labeled 125I-T3 (T3*) or 125I-T4 (T4*) via implanted minipumps. Liver, kidneys, complete intestine, and residual carcass homogenate extracts and sera were chromatographed on both Sephadex and HPLC. Total tissue T3* and T4* in steady state were assessed and corrected for hormone trapped in residual blood in tissues using radioactive albumin as a vascular marker. Labeled Triac* and/or Tetrac* were prominent among steady state metabolites of T3* or T4* in intestines, feces, and residual carcass, but not in liver or kidneys. Extrathyroidal steady state T3 and T4 pools in residual carcass were 52.8 +/- 5.74 (SD) % of total T3 and 41.2 +/- 4.57% of total T4. The largest extrathyroidal organ pools containing exchangeable T3 and T4 were intestines, with 33.1 +/- 5.46% of total (extrathyroidal) T3 and 18.1 +/- 2.80% of total T4, whereas liver and kidney pools, previously reported as the largest measured, had only 8.0 +/- 1.45% and 2.30 +/- 0.26% of total T3 and 8.72 +/- 1.56% and 1.01 +/- 0.111% of total T4, respectively. Whole blood contained 3.6 +/- 0.385% of total T3 and 31.2 +/- 1.70% of total T4. Also, 21.3 +/- 8.2% of total body T4 was converted to T3 in the whole rat (residual carcass+intestines+liver+kidneys), the production rate of T4 was PR4 = 37.1 +/- 5.28 ng/h.100 g BW and the plasma appearance rate of T3 (PR3min) was 10.6 +/- 2.83 ng/h.100 g BW. T3 production from extrathyroidal T4 was CR3-4 = 6.63 +/- 0.94 ng/h and the minimum T3 secretion rate (SR3min) was 4.02 +/- 0.94 ng/h, each per 100 g BW, indicating that the thyroid secretes more than 38% of whole body T3 in euthyroid steady state. T4 and T3 plasma clearance rates (PCR) were 1.01 +/- 0.29 (T4) and 23.7 +/- 4.36 (T3) ml/h.100 g BW.(ABSTRACT TRUNCATED AT 400 WORDS)

Influence of Iodine-Deficiency on Thyroid Hormones Homeostasis in Rats

“…[ 13 C 9 ] T 4 was administered intravenously to ID and control rats at a dose of 1.5 µg/500 µL/kg (n=5). The dose was determined based on the relative distribution and steady state pool sizes of T 4 in blood and extra-thyroidal tissues 12) and the detection limit of our method. Serum samples were obtained 5 min after [ 13 C 9 ] T 4 administration, then every 24 h for 2 weeks.…”

Triiodothyroacetic acid in health and disease

“…These findings were confirmed by incubation of rat kidney mitochondrial Robbins & Rall (1955), Christensen (1960), Green & Ingbar (1961), Oppenheimer (1974), Burger & Vallotton (1975), Chopra (1976), Gavin et al (1977Gavin et al ( , 1980, Nakamura et al (1978), Burger et al (1979), Pittman et al (1980), , Menegay et al (1989), Lopresti & Dlott (1992) TA 3 42-244 40 6.5 ± 0.5 222-298 TTR 5.2 ± 1.5 Rat T 3 768 -~2 4.2-7.3 2.3-2.5 Wilkinson et al (1959), Gosling et al (1976, Cavalieri et al (1984), Nguyen et al (1993), Liang et al (1997) TA 3 402 -~1 3.5-9.4…”

Influence of thyroidectomy on thyroxine metabolism and turnover rate in rats

“…[ 13 C 9 ]T 4 was administered intravenously to Tx and control rats at a dose of 1 . 5 mg/500 ml/kg (nZ5; Nguyen et al 1993). Serum samples were obtained 5 min after [ 13 C 9 ]T 4 administration, then at every 24 h for 2 weeks, and every 48 h for an additional week.…”