Steen solution protects pulmonary microvascular endothelial cells and preserves endothelial barrier after lipopolysaccharide-induced injury

Ta, Huy Q.; Teman, Nicholas R.; Krön, Irving L.; Roeser, Mark E.; Laubach, Victor E.

doi:10.1016/j.jtcvs.2022.04.005

Cited by 7 publications

(5 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Чаще всего в работах различных авторов упоминается о растворе Стина (Steen TM ), который применяется в большинстве протоколов перфузии. Он обладает оптимальной осмолярностью, высоким содержанием декстрана и необходимыми антиоксидантными свойствами, что обеспечивает защиту эндотелия сосудов легких от макрофагов и активации лейкоцитов [13,14].…”

Section: методика экстракорпоральной нормотермической перфузии легкихunclassified

“…Он применяется в протоколе Лунда и Торонто. Это сбалансированная, с оптимальной осмолярностью, высоким содержанием декстрана и антиоксидантными свойствами буферизованная внеклеточная среда [13,14,22]. Раствор также имеет физиологические уровни электролитов для стабилизации эндотелия: обязателен низкий уровень калия, так как высокое его содержание деполяризует мембранный потенциал и повышает процессы перекисного окисления с образованием активных форм кислорода, что оказывает негативное влияние на функционирование трансплантата [24].…”

Section: перфузионный раствор используемый при экстракорпоральнойunclassified

“…Результаты данных исследований демонстрируют, что раствор значительно снижает вызванные ишемической реперфузией эндотелиальное воспаление и окислительный стресс, а также дисфункцию эндотелиального барьера. Таким образом, данные ряда проведенных исследований говорят о том, что Steen Solution сохраняет легочную эндотелиальную барьерную функцию, способствуя противовоспалительному воздействию путем регуляции окислительных процессов [14].…”

Section: перфузионный раствор используемый при экстракорпоральнойunclassified

See 2 more Smart Citations

Ex vivo lung perfusion in lung transplantation

Fabrika,

Tychina,

Bayramkulov

et al. 2024

Transplantologiâ (Mosk.)

View full text Add to dashboard Cite

Introduction. The number of lung transplants performed worldwide is not enough because of a shortage of suitable (ideal) donors, missed chances to use lungs from donors who died of cardiac arrest, the lack of resources to perform this technically complex operation in poor, developing countries and due to a number of other reasons.) The world literature sources contain information about an increase in the number of lung transplantations by using organs from non-ideal (suboptimal and marginal) donors. This became possible thanks to the technology of ex vivo normothermic perfusion of donor lungs.Aim. To demonstrate the possibilities in the assessment, therapy and restoration of the function of non-ideal (suboptimal and marginal) donor lungs by using the technique of ex vivo lung perfusion.Material and methods. We reviewed scientific articles published in the period from 2003 to 2023 in the PubMed and Google Scholar databases for the key query "ex vivo lung perfusion".Conclusion. The ex vivo lung perfusion technique is a promising and effective procedure for lung evaluation, recondition and regeneration for) transplantation. A rapid development of technologies for this treatment modality makes it possible to increase the number of lungs suitable for transplantation, reduce the number of post-transplant complications and mortality rates on the waiting list, and improve the outcomes of lung transplantations.

show abstract

Section: методика экстракорпоральной нормотермической перфузии легкихunclassified

Section: перфузионный раствор используемый при экстракорпоральнойunclassified

See 1 more Smart Citation

Ex vivo lung perfusion in lung transplantation

Fabrika,

Tychina,

Bayramkulov

et al. 2024

Transplantologiâ (Mosk.)

View full text Add to dashboard Cite

show abstract

“…Overall, increases in pulmonary edema and extravascular lung water have a direct negative correlative effect on lung organ quality. Steen is known for its protective effect on the pulmonary microvascular endothelium [ 48 ]. Saline and Cell culture media both induced pulmonary edema and lung injury.…”

Section: Bioimpedance For Non-invasive Tissue Evaluationmentioning

confidence: 99%

Real-Time Monitoring Using Multiplexed Multi-Electrode Bioelectrical Impedance Spectroscopy for the Stratification of Vascularized Composite Allografts: A Perspective on Predictive Analytics

et al. 2023

View full text Add to dashboard Cite

Vascularized composite allotransplantation addresses injuries to complex anatomical structures such as the face, hand, and abdominal wall. Prolonged static cold storage of vascularized composite allografts (VCA) incurs damage and imposes transportation limits to their viability and availability. Tissue ischemia, the major clinical indication, is strongly correlated with negative transplantation outcomes. Machine perfusion and normothermia can extend preservation times. This perspective introduces multiplexed multi-electrode bioimpedance spectroscopy (MMBIS), an established bioanalytical method to quantify the interaction of the electrical current with tissue components, capable of measuring tissue edema, as a quantitative, noninvasive, real-time, continuous monitoring technique to provide crucially needed assessment of graft preservation efficacy and viability. MMBIS must be developed, and appropriate models explored to address the highly complex multi-tissue structures and time-temperature changes of VCA. Combined with artificial intelligence (AI), MMBIS can serve to stratify allografts for improvement in transplantation outcomes.

show abstract

“…74 Instead of standard albumin, the Ohio group suggested the use of polymetric human serum albumin because of its ability to maximize intravascular retention in limiting pulmonary edema. 75 Other than antiedematous properties, recent studies uncovered protective effects of Steen on pulmonary endothelial barrier function, 76 and additional cytoprotective and antioxidant properties via inhibition of NADPH oxidase activity. 77 Because of the high costs of Steen, low-cost alternatives, such as Krebs-Henseleit-Albumin, the Papworth-Blood, and Custadiol-MP solutions have been successfully used in EVLP settings.…”

Section: Introductionmentioning

confidence: 99%

Technical Advances Targeting Multiday Preservation of Isolated Ex Vivo Lung Perfusion

Iskender

2024

Transplantation

View full text Add to dashboard Cite

Indications for ex vivo lung perfusion (EVLP) have evolved from assessment of questionable donor lungs to treatment of some pathologies and the logistics. Yet up to 3 quarters of donor lungs remain discarded across the globe. Multiday preservation of discarded human lungs on EVLP platforms would improve donor lung utilization rates via application of sophisticated treatment modalities, which could eventually result in zero waitlist mortality. The purpose of this article is to summarize advances made on the technical aspects of the protocols in achieving a stable multiday preservation of isolated EVLP. Based on the evidence derived from large animal and/or human studies, the following advances have been considered important in achieving this goal: ability to reposition donor lungs during EVLP; perfusate adsorption/filtration modalities; perfusate enrichment with plasma and/or donor whole blood, nutrients, vitamins, and amino acids; low-flow, pulsatile, and subnormothermic perfusion; positive outflow pressure; injury specific personalized ventilation strategies; and negative pressure ventilation. Combination of some of these advances in an automatized EVLP device capable of managing perfusate biochemistry and ventilation would likely speed up the processes of achieving multiday preservation of isolated EVLP.

show abstract

Steen solution protects pulmonary microvascular endothelial cells and preserves endothelial barrier after lipopolysaccharide-induced injury

Cited by 7 publications

References 38 publications

Ex vivo lung perfusion in lung transplantation

Ex vivo lung perfusion in lung transplantation

Real-Time Monitoring Using Multiplexed Multi-Electrode Bioelectrical Impedance Spectroscopy for the Stratification of Vascularized Composite Allografts: A Perspective on Predictive Analytics

Technical Advances Targeting Multiday Preservation of Isolated Ex Vivo Lung Perfusion

Contact Info

Product

Resources

About