Stem bromelain—A new protease preparation from pineapple plants

Heinicke, R. M.; Gortner, Willis A.

doi:10.1007/bf02860437

Cited by 104 publications

(44 citation statements)

References 9 publications

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“…The mature pineapple stem contains the highest concentration of bromelain (Heinicke and Gortner 1957). However, pineapple wastes (peel, core, crown, and leaves), which are a by-product in the pineapple processing industry, are also rich in bromelain and are still underutilized.…”

Section: Purification Strategies Of Bromelainmentioning

confidence: 99%

“…Heinicke and Gortner (1957) and Soares et al (2011) reported the use of acetone and ethanol as the precipitating agents, respectively. Surprisingly, ethanol precipitation yielded 2.07-fold purification, compared to 4.44-fold obtained by ammonium precipitation (Soares et al 2011).…”

Section: Precipitationmentioning

confidence: 99%

“…This growth is entrenched with the rising general awareness of the need to protect the environment from the impact of chemical industrialization. Recently, bromelain has drawn attention in diverse industrial applications owing to its properties and higher commercial values (Heinicke and Gortner 1957). Bromelain is a protease derived from the stem and fruit of pineapples (Ananas comosus).…”

Section: Introductionmentioning

confidence: 99%

“…Stem bromelain can be isolated from stem juices by means of precipitation and centrifugation (Devakate et al 2009;Heinicke and Gortner 1957). Moreover, using chromatographic methods, other basic proteolytic (ananain, comosain, F4, F5, and F9) and acidic components of stem bromelain fraction A (SBA/a) and fraction B (SBA/b) have been partially and fully purified (Feijoo-Siota and Villa 2011;Harrach et al 1995Harrach et al , 1998Murachi et al 1964;Napper et al 1994;Rowan et al 1990;Wharton 1974).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Bromelain: an overview of industrial application and purification strategies

Arshad

Amid

Yusof

et al. 2014

Appl Microbiol Biotechnol

169

134

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This review highlights the use of bromelain in various applications with up-to-date literature on the purification of bromelain from pineapple fruit and waste such as peel, core, crown, and leaves. Bromelain, a cysteine protease, has been exploited commercially in many applications in the food, beverage, tenderization, cosmetic, pharmaceutical, and textile industries. Researchers worldwide have been directing their interest to purification strategies by applying conventional and modern approaches, such as manipulating the pH, affinity, hydrophobicity, and temperature conditions in accord with the unique properties of bromelain. The amount of downstream processing will depend on its intended application in industries. The breakthrough of recombinant DNA technology has facilitated the large-scale production and purification of recombinant bromelain for novel applications in the future.

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Section: Purification Strategies Of Bromelainmentioning

confidence: 99%

Section: Precipitationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bromelain: an overview of industrial application and purification strategies

Arshad

Amid

Yusof

et al. 2014

Appl Microbiol Biotechnol

169

134

View full text Add to dashboard Cite

show abstract

“…The enzyme extracted from the stem is called stem Bromelain (EC 3.4.22.32) and from fruit is called fruit Bromelain (EC 3.4.22.33) (Babu et al, 2008). It is made up of 212 amino acids and the molecular weight is 33 kDa (Heinicke and Gortner, 1957;Murachi and Neuratii, 1960). Bromelain is inhibited by Hg ++ , Ag + , Cu ++ , antitrypsin, estatin A and B, iodoacetate (Harrach et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Optimisation of Bromelain Enzyme Extraction from Pineapple (<i>Ananas comosus</i>) and Application in Process Industry

Mohan

Sivakumar

Rangasamy

et al. 2016

American Journal of Biochemistry and Biotechnology

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Bromelain is a general name for a family of sulfhydryl proteolytic enzymes obtained from Ananas comosus, the pineapple plant. The objective of the present study is to compare the activity of Bromelain from pineapple fruit and peel and to check the efficiency of this crude Bromelain enzyme in unhairing step in leather processing. The crude Bromelain was extracted and activity of enzyme was measured using tyrosine as standard and expressed in Units/mL of enzyme. The effect of preservatives such as sodium benzoate and sodium metabisulphite on the activity of the enzyme was studied for one week. The effect of pH (3-5) was studied using suitable pH buffer solutions. The kinetics of the proteolytic activity of the enzyme was studied using casein as substrate. It was found that the optimum pH of the crude extract was 4.5. In general enzyme activity got affected by the use of preservatives. The kinetic studies show that the activity of enzyme was increasing during initial period. The unhairing activity of crude extract of pH 4.5 was also studied using cow hide pieces. After 24 h treatment with the extracted crude Bromelain enzyme, good unhairing efficacy was achieved for leather making.

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Biology and postharvest physiology of pineapple

Lobo

Yahia

2016

Handbook of Pineapple Technology

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Stem bromelain—A new protease preparation from pineapple plants

Cited by 104 publications

References 9 publications

Bromelain: an overview of industrial application and purification strategies

Bromelain: an overview of industrial application and purification strategies

Optimisation of Bromelain Enzyme Extraction from Pineapple (<i>Ananas comosus</i>) and Application in Process Industry

Biology and postharvest physiology of pineapple

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