Stem Cell-Based (Auto)Grafting: From Innovative Research Toward Clinical Use in Regenerative Medicine

Balint, Bela; Obradović, Slobodan; Todorović, Milena; Pavlović, Mirjana; Mihaljević, Biljana

doi:10.5772/54473

Cited by 3 publications

(63 citation statements)

References 50 publications

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“…They are also capable of differentiation into all cell types -that is have an option to "switch" into different cell lineages. Thus, inside SC compartment, embryonic SCs are the most "promising", but also the most controversial cell category (2)(3)(4)(5)(6).…”

Section: Stem Cells -Biology and Subtypesmentioning

confidence: 99%

“…SCs are thought to reside in a specific area of tissues ("niche") where they may remain inactive ("non-dividing") for many years until they are activated by some disease or tissue injury. They can be found in the bone marrow (BM), peripheral blood (PB), blood vessels, fat tissue, skeletal muscles, skin, liver, etc (3)(4)(5)(6)(7)(8)(9)(10)(11). Typically, adult SCs are capable of making identical copies (self-renewal) and generate cell types of the tissue in which they reside.…”

Section: Stem Cells -Biology and Subtypesmentioning

confidence: 99%

“…Typically, adult SCs are capable of making identical copies (self-renewal) and generate cell types of the tissue in which they reside. However, a number of investigations over the last two decades have raised the possibility that SCs from one tissue may be able to give rise to cell types of some different tissue ("SC-plasticity") (2)(3)(4)(5)(6).…”

Section: Stem Cells -Biology and Subtypesmentioning

confidence: 99%

“…Hematopoietic SCs could be characterized as cells having an extensive, but well-balanced self-renewal potential, proliferative and differentiation capacity, as well as ability for cell plasticity. Various populations of SCs expresses CD34 antigen, consequently they are named also as CD34 + cells (2,5,11). In the BM about 2-4% of total nucleated cells (TNCs) express the CD34 antigen.…”

Section: Stem Cells -Biology and Subtypesmentioning

confidence: 99%

“…Immature (more primitive) SC ish expressed CD90 antigen, which is also exposed by 1 -4% of fetal liver cells, as well as umbilical cord blood (UCB), BM and few PB cells. These cells (CD34 + /CD90 + subtype, named as CD90 + SC ish ) are responsible for stable and long-term BM repopulation (engraftment) with complete hematopoietic reconstitution (2,5,9).…”

Section: Stem Cells -Biology and Subtypesmentioning

confidence: 99%

See 4 more Smart Citations

From nucleated to ex vivo manipulated stem cells: An updated biological and clinical synopsis

Balint¹,

Pavlović²,

Todorović³

2020

Medicinska reč

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Hematopoietic stem cells (SCs) are responsible for the production and replacement (proliferation) of an extensive quantity of functionally competent blood cells (differentiation) during the entire life, while simultaneously maintaining the ability to reproduce themselves (self-renewal). A complex network of interactive substances and factors organize and protect the survival, maturation and multiplication of SCs. Hemobiological events in the bone marrow (BM) are synchronized and balanced by the extracellular matrix and microenvironment provided by stromal cells. These cells-including macrophages, fibroblasts, dendritic, endothelial and other cells-stimulate SCs by producing specific hematopoietic growth factors. Other cytokines secreted by stromal cells regulate the adhesion molecules positioned on SCs, allowing them to remain in the BM or migrate to an area where the respective cell type is needed. Thus, hematopoietic SCs could be defined as cells with high proliferative capacity and extensive potential to differentiate into all blood cells or some somatic cell types (SC plasticity)-such as cardiomyocytes, myocytes, osteocytes, chondrocytes, hepatocytes, and even endothelial cells. Recent increasing clinical use of cell-mediated therapeutic approaches has resulted in increased needs for SCs, but in superior operating procedures during their ex vivo manipulations. The aim of cell harvestings is to obtain a higher SC yield and improved viability or clonogenicity. The goal of optimized cryoinvestigation protocols is to get a minimized cell damages (cryoinjury). Despite the fact that different SC collection protocols and cell freezing practice are already in routine use, a lot of questions related to the optimal SC ex vivo manipulations are still unresolved. This review summarizes fundamental knowledge and methodological approaches, and recapitulates data enabling progress on constantly evolving research frontiers in the SC area. The studies (including also our investigations) that evaluated the efficiency and safety of SC-treatment (transplants and regenerative medicine) will be also concisely presented.

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Section: Stem Cells -Biology and Subtypesmentioning

confidence: 99%

Section: Stem Cells -Biology and Subtypesmentioning

confidence: 99%

Section: Stem Cells -Biology and Subtypesmentioning

confidence: 99%

Section: Stem Cells -Biology and Subtypesmentioning

confidence: 99%

Section: Stem Cells -Biology and Subtypesmentioning

confidence: 99%

See 3 more Smart Citations

From nucleated to ex vivo manipulated stem cells: An updated biological and clinical synopsis

Balint¹,

Pavlović²,

Todorović³

2020

Medicinska reč

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Stem cells: Haemobiology and clinical data summarising: A critical review

Balint¹,

Pavlović²,

Todorović³

2020

Scripta Medica

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Stem cells (SC) are the unique and "key-cells" in the human body "working" as a source of producing a large number (proliferation) of mature (differentiation) cells inside different tissues ("cytopoiesis") - while at the same time maintaining the ability to "reproduce" themselves (self-renewal). These events are balanced by interactive signals from the extracellular matrix, as well as microenvironment provided by stromal cells. On the other hand, SC plasticity (so-called "inter-systemic plasticity") is the ability of the most "primitive" (immature) adult SCs to switch to novel identities. The phrase SC plasticity also involves phenotypic potential of these cells, broader than spectrum of phenotypes of differentiated cells in their original tissues. Recent increasing clinical use of cell-mediated therapeutic approaches has resulted in enlarged needs for both, higher quantity of SCs and improved operating procedures during extracorporeal manipulations. The aim of harvesting procedures is to obtain the best SC yield and viability. The goal of optimised cryopreservation is to minimise cellular thermal damages during freeze/thaw process (cryoinjury). Despite the fact that different SC collection, purification and cryopreservation protocols are already in routine use - a lot of problems related to the optimal SC extracorporeal manipulations are still unresolved. The objective of this paper is to provide an integral review of early haemobiological and cryobiological research in the unlimited "SC-field" with emphasis on their entities, recent cell-concepts, extracorporeal manipulative and "graft-engineering" systems. Their therapeutic relevance and efficacy in "conventional" SC transplants or regenerative medicine will be briefly summarised. Finally, in this paper original results will not be pointed out - related to neither SC transplants nor regenerative medicine - but a light will be shed on some of them.

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A stem cell overview: From evolving hemobiological concepts to (auto)grafting in clinical practice

Balint¹,

Pavlović²,

Marković³

et al. 2022

Srpski medicinski časopis Lekarske komore

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Conventional hematopoietic stem cell transplantation is a well-known treatment method for numerous acquired and congenital hematopoietic disorders, disorders of the immune system, as well as certain metabolic disorders. Stem cells (SCs) can be defined as cells capable of self-renewal with a high proliferative capacity and the potential to differentiate into functionally competent mature cells. Stem cells can be divided into embryonic SCs (ESCs) and tissue-specific or adult SCs - such as bone marrow (BM) stem cells, peripheral blood (PB) stem cells, and SCs derived from umbilical cord blood (UCB), as well as other non-hematopoietic or somatic SCs. SCs in adults are characteristically considered to be restricted in their regenerative and differentiative potential, while embryonic stem cells are 'true' totipotent/pluripotent cells, due to their ability to develop into endoderm, ectoderm, or mesoderm - all three embryonic tissue types in the human body. They are the most promising, but also the most controversial type of potentially transplantable SCs. Immature hematopoietic SCs have the potential of differentiating, not only into all blood cells, but also into some somatic cell types (SC plasticity). In different clinical settings, the transplantation of immature stem cells leads to the repopulation of recipient bone marrow, with subsequent complete, stable, and long-term reconstitution of hematopoiesis. Given that immature stem cells are also capable of homing to different tissues, autologous stem cell implantation into a damaged and/or ischemic area induces their colonizing and consecutive transdifferentiating into cell lineages of the host organ, including neovascularization. Thus, they are clinically applicable in the field of regenerative medicine for the treatment of myocardial, brain, vascular, liver, pancreatic, and other tissue damage. The purpose of this overview is to recapitulate the key developments in the rapidly evolving area of stem cell research, as well as to review the use of SCs in conventional transplantations and in regenerative medicine. Additionally, a brief critical evaluation of our own stem cell research will be summarized.

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Stem Cell-Based (Auto)Grafting: From Innovative Research Toward Clinical Use in Regenerative Medicine

Cited by 3 publications

References 50 publications

From nucleated to ex vivo manipulated stem cells: An updated biological and clinical synopsis

From nucleated to ex vivo manipulated stem cells: An updated biological and clinical synopsis

Stem cells: Haemobiology and clinical data summarising: A critical review

A stem cell overview: From evolving hemobiological concepts to (auto)grafting in clinical practice

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