Stem-cell-capturing collagen scaffold promotes cardiac tissue regeneration

Shi, Chunying; Li, Qingguo; Zhao, Yannan; Chen, Wei; Chen, Bing; Xiao, Zhifeng; Lin, Hang; Nie, Ling; Wang, Dongjin; Dai, Jianwu

doi:10.1016/j.biomaterials.2010.12.026

Cited by 107 publications

(89 citation statements)

References 35 publications

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“…Shi et al . investigated this concept by covalently conjugating the anti-stem cell antigen-1 (Sca-1) monoclonal antibody on the surface of collagen scaffolds [54]. This antibody was selected since Sca-1 protein is the most common surface marker expressed in hematopoietic, cardiac, and skeletal muscle stem cells [55].…”

Section: Strategies To Achieve Stem and Progenitor Cell Recruitmentmentioning

confidence: 99%

Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration

Pacelli

Basu

Whitlow

et al. 2017

Advanced Drug Delivery Reviews

132

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A leading strategy in tissue engineering is the design of biomimetic scaffolds that stimulate the body’s repair mechanisms through the recruitment of endogenous stem cells to sites of injury. Approaches that employ the use of chemoattractant gradients to guide tissue regeneration without external cell sources are favored over traditional cell-based therapies that have limited potential for clinical translation. Following this concept, bioactive scaffolds can be engineered to provide a temporally and spatially controlled release of biological cues, with the possibility to mimic the complex signaling patterns of endogenous tissue regeneration. Another effective way to regulate stem cell activity is to leverage the inherent chemotactic properties of extracellular matrix (ECM)-based materials to build versatile cell-instructive platforms. This review introduces the concept of endogenous stem cell recruitment, and provides a comprehensive overview of the strategies available to achieve effective cardiovascular and bone tissue regeneration.

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Section: Strategies To Achieve Stem and Progenitor Cell Recruitmentmentioning

confidence: 99%

Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration

Pacelli

Basu

Whitlow

et al. 2017

Advanced Drug Delivery Reviews

132

View full text Add to dashboard Cite

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“…Thus, introduction of Cys into the polymeric chain through genetic engineering allows for unique control over the location and the number of the chemically functional thiols in each collagen molecule. Previous studies have introduced sulfhydryls to collagen using Trauts reagent, which reacts with primary amines; 47 however, this strategy does not enable control over the number or locations of reactive sites. This lack of control could potentially lead to heterogeneity of cross-links or immobilized factors, resulting in heterogeneous local properties (e.g., irregular porosity or mechanics, local gradients of growth factor) within the scaffold.…”

Section: Discussionmentioning

confidence: 99%

“…We demonstrated that collagen variants with TGF-β attached to non-native cysteines induced αSMA expression in NIH/3T3 fibroblasts to a greater degree than collagen without the cysteines, supporting a greater extent of myofibroblast differentiation. Other bioactive molecules, such as antibodies for cell capture 47 or VEGF to promote vascularization, 52 could be utilized in lieu of TGF-β to customize the cellular microenvironment.…”

Section: Discussionmentioning

confidence: 99%

Expanding Functionality of Recombinant Human Collagen Through Engineered Non-Native Cysteines

et al. 2014

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Collagen is the most abundant protein in extracellular matrices and is commonly used as a tissue engineering scaffold. However, collagen and other biopolymers from native sources can exhibit limitations when tuning mechanical and biological properties. Cysteines do not naturally occur within the triple-helical region of any native collagen. We utilized a novel modular synthesis strategy to fabricate variants of recombinant human collagen that contained 2, 4, or 8 non-native cysteines at precisely defined locations within each biopolymer. This bottom-up approach introduced capabilities using sulfhydryl chemistry to form hydrogels and immobilize bioactive factors. Collagen variants retained their triple-helical structure and supported cellular adhesion. Hydrogels were characterized using rheology, and the storage moduli were comparable to fibrillar collagen gels at similar concentrations. Furthermore, the introduced cysteines functioned as anchoring sites, with TGF-β1-conjugated collagens promoting myofibroblast differentiation. This approach demonstrates the feasibility to produce customdesigned collagens with chemical functionality not available from native sources.

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“…Porous collagen has been used for many types of tissue engineering, such as cardiac and cartilaginous tissue engineering, and the pore size of porous collagen can be easily controlled for cell attachment, penetration and proliferation. In addition, the open and connected pores in porous collagen allow cells to rapidly grow into the scaffold, after which the scaffold is digested by enzymes and replaced by ECM produced by cells [16][17][18]. Gel-like collagen has been used to treat and improve soft-tissue defects (e.g., via skin regeneration) by injection due to its high plasticity and nonporous structure.…”

Section: Introductionmentioning

confidence: 99%

Characterization and implantation of a novel foamy type of collagen into SD rats to regenerate tissue by slowing down the collagen degradation rate

Yao

Liao

Liang

2016

International Journal of Polymeric Materials and Polymeric Biom

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2016) Characterization and implantation of a novel foamy type of collagen into SD rats to regenerate tissue by slowing down the collagen degradation rate, International Journal of Polymeric Materials and Polymeric Biomaterials, 65:10, 508-515To link to this article: http://dx. ABSTRACTCollagen has high biocompatibility and biodegradability and therefore is an ideal natural polymer biomaterial for tissue regeneration, such as gel-like and porous collagens. However, the limitation of gel-like collagen is unsuitability for cell/tissue ingrowth and the limitation of porous collagen is quick degradation rate. Here, the authors propose a novel type of foamy collagen to address the previous limitations. Foamy collagen with a closed/nonconnective porous structure was formed using foaming technology and not using toxic crosslinking reagents. This research aimed to investigate the macro-/ microstructure, the in vitro/vivo degradation rate, and the tissue regeneration feasibility of foamy collagen. For in vitro degradation rate, porous collagen was completely degraded by enzyme, whereas 91.5% and 72.1% of gel-like and foamy collagens, respectively, remained intact. In vivo degradation rate had a similar trend as in vitro data. After implantation of the collagens in Sprague Dawley rats, immune cells were observed at the periphery of the three types of collagen at day 3. Fibroblast ingrowth was observed in foamy and porous collagen groups at day 7. Neocapillary formation and tissue regeneration were observed in foamy and porous collagen groups at day 14, but nearly none in gel-like collagen group. In conclusion, the authors believe that foamy collagen is promising for application of soft-tissue regeneration. GRAPHICAL ABSTRACT ARTICLE HISTORY

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Stem-cell-capturing collagen scaffold promotes cardiac tissue regeneration

Cited by 107 publications

References 35 publications

Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration

Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration

Expanding Functionality of Recombinant Human Collagen Through Engineered Non-Native Cysteines

Characterization and implantation of a novel foamy type of collagen into SD rats to regenerate tissue by slowing down the collagen degradation rate

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