Stem Cell Culture Under Simulated Microgravity

Anıl-İnevi, Müge; Sarigil, Oyku; Kizilkaya, Melike; Meşe, Gülistan; Tekin, H. Cumhur; Özçivici, Engin

doi:10.1007/5584_2020_539

Cited by 11 publications

(25 citation statements)

References 133 publications

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“…In the experimental group, a rotating cell culture system (RCCS-4D, SYNTHECON) was used to mimic some aspects of microgravity on the encapsulated follicles 14 . The underlying principle of the RCCS is that an increase in the rate of rotation will result in a decrease in the perimeter of the circular path 58 . The microgravity condition is simulated when the Matrigel/follicles eventually begin to rotate around their own axis.…”

Section: Methodsmentioning

confidence: 99%

Simulated microgravity reduces quality of ovarian follicles and oocytes by disrupting communications of follicle cells

et al. 2023

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Ovarian follicles are the fundamental structures that support oocyte development, and communications between oocytes and follicle somatic cells are crucial for oogenesis. However, it is unknown that whether exposure to microgravity influences cellular communications and ovarian follicle development, which might be harmful for female fertility. By 3D culturing of ovarian follicles under simulated microgravity (SMG) conditions in a rotating cell culture system, we found that SMG treatment did not affect the survival or general growth of follicles but decreased the quality of cultured follicles released oocytes. Ultrastructure detections by high-resolution imaging showed that the development of cellular communicating structures, including granulosa cell transzonal projections and oocyte microvilli, were markedly disrupted. These abnormalities caused chaotic polarity of granulosa cells (GCs) and a decrease in oocyte-secreted factors, such as Growth Differentiation Factor 9 (GDF9), which led to decreased quality of oocytes in these follicles. Therefore, the quality of oocytes was dramatically improved by the supplementations of GDF9 and NADPH-oxidase inhibitor apocynin. Together, our results suggest that exposure to simulated microgravity impairs the ultrastructure of ovarian follicles. Such impairment may affect female fertility in space environment.

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Section: Methodsmentioning

confidence: 99%

Simulated microgravity reduces quality of ovarian follicles and oocytes by disrupting communications of follicle cells

et al. 2023

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“…In clinostats, the sample holder size is kept in millimeter scale to minimize acceleration, but limiting the capacity of the sample volume [80]. In RPMs, the addition of the vertical rotation axis generates acceleration in all directions, allowing for more sample volume [81]. Overall, RPM has more flexibility in selecting the parameters of the sample holder, which makes RPM more suitable for holding LOC devices.…”

Section: Random Positioning Machinementioning

confidence: 99%

Lab-On-a-Chip Technologies for Microgravity Simulation and Space Applications

Vashi¹,

Sreejith²,

Nguyen³

2022

Preprint

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Gravity played an important role in the development of life on earth. The effect of gravity on a living organisms can be investigated by controlling the magnitude of gravity. Most reduced gravity experiments are conducted on the lower earth orbit (LEO) in the International Space Station (ISS). However, running experiments in ISS face challenges such as high cost, extreme condition, lack of direct accessibility, and long waiting period. Therefore, researchers have developed various ground-based devices and methods to perform reduced gravity experiments. However, the search for life outside the earth requires more intensive research. Advancements in conventional methods and the development of new tools are required for this purpose. The advantages of Lab-on-a-Chip (LOC) devices make them an attractive option for simulating microgravity. This paper briefly reviews the advancement of LOC technologies for simulating microgravity in a earth-based laboratory.

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“…In clinostats, the sample holder size is kept in millimeter scale to minimize acceleration but limiting the capacity of the sample volume [ 82 ]. In RPMs, the addition of the vertical rotation axis generates acceleration in all directions, allowing for more sample volume [ 83 ]. Overall, RPM has more flexibility in selecting the parameters of the sample holder, which makes RPM more suitable for holding LOC devices.…”

Section: Lab-on-a-chip Technologies In Conventional Simulated Microgr...mentioning

confidence: 99%

Lab-on-a-Chip Technologies for Microgravity Simulation and Space Applications

2022

View full text Add to dashboard Cite

Gravity plays an important role in the development of life on earth. The effect of gravity on living organisms can be investigated by controlling the magnitude of gravity. Most reduced gravity experiments are conducted on the Lower Earth Orbit (LEO) in the International Space Station (ISS). However, running experiments in ISS face challenges such as high cost, extreme condition, lack of direct accessibility, and long waiting period. Therefore, researchers have developed various ground-based devices and methods to perform reduced gravity experiments. However, the advantage of space conditions for developing new drugs, vaccines, and chemical applications requires more attention and new research. Advancements in conventional methods and the development of new methods are necessary to fulfil these demands. The advantages of Lab-on-a-Chip (LOC) devices make them an attractive option for simulating microgravity. This paper briefly reviews the advancement of LOC technologies for simulating microgravity in an earth-based laboratory.

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Stem Cell Culture Under Simulated Microgravity

Cited by 11 publications

References 133 publications

Simulated microgravity reduces quality of ovarian follicles and oocytes by disrupting communications of follicle cells

Simulated microgravity reduces quality of ovarian follicles and oocytes by disrupting communications of follicle cells

Lab-On-a-Chip Technologies for Microgravity Simulation and Space Applications

Lab-on-a-Chip Technologies for Microgravity Simulation and Space Applications

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