Stem cell-derived hepatocyte therapy using versatile biomimetic nanozyme incorporated nanofiber-reinforced decellularized extracellular matrix hydrogels for the treatment of acute liver failure

Electrospun Nanofiber Scaffold for Skin Tissue Engineering: A Review

“…71 The study revealed that incorporating soluble eggshell membrane protein led to enhanced spinnability and mechanical strength of PLGA. 72…”

Reactive Oxygen Species Scavenging Nanozymes: Emerging Therapeutics for Acute Liver Injury Alleviation

“…In order to better study the mechanism and treatment of acute liver injury, different kinds of acute liver injury animal models have been established, including drug-induced liver injury (eg APAP-induced liver injury, [58][59][60] anthracyclineinduced liver injury 61 ), chemical-induced liver injury (eg CCl 4 -induced liver injury 62,63 ), immune-induced liver injury (eg lipopolysaccharide (LPS)-induced liver injury, 64,65 D-galactosamine-induced liver injury, 66 concanavalin A-induced liver injury 67,68 ), alcohol-induced liver injury, 69,70 and hepatic ischemia-reperfusion injury. 7,35,71 Transition Metal-Based Nanozymes…”

“…13 Jin et al further fabricated a synergistic therapeutic platform (HLCs/Cu NZs@fiber/dECM) consisting of Cu 5.4 O nanozymes-loaded PLGA nanofibers and decellularized extracellular matrix hydrogels for the alleviation of ALI and preventing the deterioration of hepatocytes necrosis (Figure 4B). 62…”

Microfluidic Fabrication of MicroRNA-Induced Hepatocyte-Like Cells/Human Umbilical Vein Endothelial Cells-Laden Microgels for Acute Liver Failure Treatment

“…Orthotopic liver transplantation is considered as the most recommended treatment for severe liver failure, but it is usually restricted by donor shortage and high immunosuppression from allogeneic grafts. , Transplantation of hepatocytes or hepatocyte-like cells is viewed as a promising and less invasive option to replace orthotopic liver transplants in ALF therapy by supplying rapid support. , Besides, the transplanted cells have paracrine functions to ameliorate inflammation and promote liver regeneration, thus restoring liver functions. − However, isolated primary hepatocytes are difficult to generally proliferate and maintain hepatic function in vitro . , Stem cells such as embryonic stem cells, induced pluripotent stem cells and mesenchymal stem cells have unlimited proliferation capability and can generate hepatocyte-like cells in vitro . Stem cell-induced hepatocyte-like cells have great potential for transplantation in clinical ALF treatment due to their potential to be cryopreserved and banked technically in vitro . ,, Nevertheless, it is still complicated and expensive to efficiently generate functional hepatocyte-like cells from stem cells by traditional hepatic differentiation strategies. − Excessive reactive oxygen species (ROS) in injured tissues would also damage the cell viability and survival of the transplanted cells, which also restricted therapeutic effects in ALF treatment. , …”

“…10 Stem cell-induced hepatocyte-like cells have great potential for transplantation in clinical ALF treatment due to their potential to be cryopreserved and banked technically in vitro. 3,11,12 Nevertheless, it is still complicated and expensive to efficiently generate functional hepatocyte-like cells from stem cells by traditional hepatic differentiation strategies. 13−15 Excessive reactive oxygen species (ROS) in injured tissues would also damage the cell viability and survival of the transplanted cells, which also restricted therapeutic effects in ALF treatment.…”