Stem cell factor/c-Kit signaling in in vitro cultures supports early mouse embryonic development by accelerating proliferation via a mechanism involving Akt-downstream genes

Lim, Jinho; Eum, Jin Hee; Lee, Jeoung Eun; Kim, Eun Sun; Chung, Hyung Min; Yoon, Tae Ki; Kim, Kye-Seong; Lee, Dong Ryul

doi:10.1007/s10815-010-9449-9

Cited by 14 publications

(17 citation statements)

References 31 publications

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“…It has been recently shown that early onset-ofcleavage in human embryos is a predictor of normal development and high chance of live birth when compared with late onset-of-cleavage embryos in IVF/ICSI cycles [31]. Successful implantation of early cleaving embryos may be the result, among others, of a more optimal synchronization between embryonic development and the preparation of a receptive uterine environment [32].…”

Section: Discussionmentioning

confidence: 99%

Improvement of mouse embryo quality by myo-inositol supplementation of IVF media

Colazingari

Fiorenza

Carlomagno³

et al. 2014

J Assist Reprod Genet

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Objective Myo-inositol (myoIns) has a positive role in mammalian development and human reproduction. Since experiments on farming species suggest a similar role in preimplantation development, we evaluated the hypothesis that the inclusion of myoIns in human embryo culture media would produce an increase in embryo quality in IVF cycles, using the mouse embryo assay. Methods To determine the effect of myoIns on completion of preimplantation development in vitro, one-cell embryos of the inbred C57BL/6N mouse strain were produced by ICSI, cultured in human fertilization media in the presence of myoIns (myoIns+) or in its absence (myoIns-) and evaluated morphologically. Daily progression through cleavage stages, blastocyst production and expansion and blastomere number at 96 hours post fertilization were assessed. Results Compared to myoIns-embryos, myoIns+ embryos displayed a faster cleavage rate and by the end of preimplantation development, the majority of myoIns+ blastocysts was expanded and formed by a higher number of blastomeres. Conclusion The presence of myoIns resulted in both an increase in proliferation activity and developmental rate of in vitro cultured early mouse embryos, representing a substantial improvement of culture conditions. These data may identify myoIns as an important supplement for human embryo preimplantation culture.

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Section: Discussionmentioning

confidence: 99%

Improvement of mouse embryo quality by myo-inositol supplementation of IVF media

Colazingari

Fiorenza

Carlomagno³

et al. 2014

J Assist Reprod Genet

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“…SCF is highly expressed in oviducts and uterus through early pregnancy, demonstrating the prospect of paracrine signalling to the developing embryo (Arceci et al 1992). Addition of SCF improves blastocyst formation, and silencing c-kit reduces the kinetics of blastocyst formation indicating a proliferation-inducing effect (Lim et al 2010). Since both c-fms and c-kit are tyrosine kinases that activate similar signalling pathways, there is a high likelihood of cross-compensation between the two ligand-receptor systems.…”

Section: Colony Stimulating Factor-1 and Stem Cell Factormentioning

confidence: 99%

Female Tract Cytokines and Developmental Programming in Embryos

Robertson

Chin

Schjenken

et al. 2015

Advances in Experimental Medicine and Biology

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In the physiological situation, cytokines are pivotal mediators of communication between the maternal tract and the embryo. Compelling evidence shows that cytokines emanating from the oviduct and uterus confer a sophisticated mechanism for 'fine-tuning' of embryo development, influencing a range of cellular events from cell survival and metabolism, through division and differentiation, and potentially exerting long-term impact through epigenetic remodelling. The balance between survival agents, including GM-CSF, CSF1, LIF, HB-EGF and IGFII, against apoptosis-inducing factors such as TNFα, TRAIL and IFNg, influence the course of preimplantation development, causing embryos to develop normally, adapt to varying maternal environments, or in some cases to arrest and undergo demise. Maternal cytokine-mediated pathways help mediate the biological effects of embryo programming, embryo plasticity and adaptation, and maternal tract quality control. Thus maternal cytokines exert influence not only on fertility and pregnancy progression but on the developmental trajectory and health of offspring. Defining a clear understanding of the biology of cytokine networks influencing the embryo is essential to support optimal outcomes in natural and assisted conception.

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“…mTOR is considered as a crucial factor in cellular adaptive responses to energy, nutrients and growth factors in mammals (Cetrullo, D'Adamo, Tantini, Borzi, & Flamigni, 2015). Akt can activate mTOR, which regulates cell proliferation (Lim et al, 2010). In addition, ANXA8 upregulates the mRNA expression of vEGF, which is a key regulator of angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

Annexin A8 (ANXA8) regulates proliferation of porcine endometrial cells via Akt signalling pathway

Jiang

Xue

Kang

et al. 2018

Reprod Domestic Animals

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Annexin A8 (ANXA8) gene, a member of the annexin family, encodes an anticoagulant protein involved in blood coagulation cascade and acts as an indirect inhibitor of the thromboplastin-specific complex. However, little is known about the function of ANXA8 in porcine endometrial cells so far. Here, ANXA8 mRNA was found to be abundant in porcine endometrium on days 11-13 of pregnancy. Real-time RT-PCR analysis indicated that the mRNA expression of the leukaemia inhibitory factor (LIF) and the epidermal growth factor (EGF) was upregulated by ANXA8 in porcine endometrial cells. Immunofluorescence technology and cell cycle analysis revealed that ANXA8 promoted the proliferation of endometrial cells, as evidenced by the abundant proliferating cell nuclear antigen (PCNA) expression and an increase in the S phase. Western blot analysis results indicated that ANXA8 activated the phosphorylation of the target protein kinase B (Akt) protein. Immunofluorescence technology results showed that the PCNA protein had no significant change in porcine endometrial cells with both ANXA8 overexpression and the addition of Akt inhibitor. Furthermore, the number of implantation sites was significantly reduced by injection of mus-siRNA-ANXA8 into the uterine horn of mice. Collectively, these results suggest that ANXA8 promotes the proliferation of endometrial cells through the Akt signalling pathway.

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Stem cell factor/c-Kit signaling in in vitro cultures supports early mouse embryonic development by accelerating proliferation via a mechanism involving Akt-downstream genes

Cited by 14 publications

References 31 publications

Improvement of mouse embryo quality by myo-inositol supplementation of IVF media

Improvement of mouse embryo quality by myo-inositol supplementation of IVF media

Female Tract Cytokines and Developmental Programming in Embryos

Annexin A8 (ANXA8) regulates proliferation of porcine endometrial cells via Akt signalling pathway

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