Stem cell niches functionalized strategies for organ regeneration and manufacturing

Liu, Huan; Hu, Lei; Zhang, Dake; Wang, Xiaogang; Wang, Songlin

doi:10.59717/j.xinn-med.2023.100037

Cited by 4 publications

(1 citation statement)

References 139 publications

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“…43 It should be noted that engineering techniques can improve the efficacy of organoid modeling by utilizing concentration gradients. For example, the extrusion-printed kidney organoid, 44 the cube method, 45 or microfluidics for creating concentration gradient, which shows parallels with organoids-on-chip discussed later.…”

Section: The Single Organoid Is the Elemental Basismentioning

confidence: 99%

Converging neural-centric and mechano-regulation in organoid modeling for system biology and medicine

Tang,

Wang,

Khutsishvili

et al. 2024

TIME

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<p>The understanding of complex biological systems and the development of effective precision medicine strategies necessitate controllable and tractable experimental models. The human body is composed of systemic and systematic interactions at multiple levels such as occurs between cells, tissues, and organs. Hence, how to recapitulate the system complexity of the human body has become an inevitable problem. This review emphasizes the need to understand complex interactions between organs by exploring the potential use of organoids and their derivatives. We focus on the nervous system and its pivotal roles in the regulation of peripheral organs, and meanwhile, highlight the importance of often overlooked mechanobiological factors. The nervous system controls many neuromodulation processes and is capable of transmitting biological information through electrophysiology. In addition, mechano-regulation operates at the cellular and microenvironment levels, functioning through system-level regulation. It can influence neural tissue or collaborate with nerves to direct skin and visceral tissue responses and their immunity. To achieve <i>in situ</i> probing and manipulation of such processes, we recommend the use of organoid assembloids that directly fusion individual organoids to create interactive structures of neural-centric complexes and mechano-regulation conditions, or organoids-on-a-chip that relies on microfluidic chips as tailorable bioreactors to form multi-organ associations and simulate and incorporate mechanobiological and neurological regulations. Based on mechano-regulatory influence at the cellular and microenvironment levels, we may develop more systematic, biomimetic, and robust in-vitro models. These models not only approach genuine physiology and pathology in humans without sacrificing real-time observation capabilities but present minimal ethical concerns and offer substantial potential for industrial scalability.</p>

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Section: The Single Organoid Is the Elemental Basismentioning

confidence: 99%

Converging neural-centric and mechano-regulation in organoid modeling for system biology and medicine

Tang,

Wang,

Khutsishvili

et al. 2024

TIME

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A Novel Superparamagnetic‐Responsive Hydrogel Facilitates Disc Regeneration by Orchestrating Cell Recruitment, Proliferation, and Differentiation within Hostile Inflammatory Niche

Xue,

Peng,

Zhang

et al. 2024

Advanced Science

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In situ disc regeneration is a meticulously orchestrated process, which involves cell recruitment, proliferation and differentiation within a local inflammatory niche. Thus far, it remains a challenge to establish a multi‐staged regulatory framework for coordinating these cellular events, therefore leading to unsatisfactory outcome. This study constructs a super paramagnetically‐responsive cellular gel, incorporating superparamagnetic iron oxide nanoparticles (SPIONs) and aptamer‐modified palladium‐hydrogen nanozymes (PdH‐Apt) into a double‐network polyacrylamide/hyaluronic acid (PAAm/HA) hydrogel. The Aptamer DB67 within magnetic hydrogel (Mag‐gel) showed a high affinity for disialoganglioside (GD2), a specific membrane ligand of nucleus pulposus stem cells (NPSCs), to precisely recruit them to the injury site. The Mag‐gel exhibits remarkable sensitivity to a magnetic field (MF), which exerts tunable micro/nano‐scale forces on recruited NPSCs and triggers cytoskeletal remodeling, consequently boosting cell expansion in the early stage. By altering the parameters of MF, the mechanical cues within the hydrogel facilitates differentiation of NPSCs into nucleus pulposus cells to restore disc structure in the later stage. Furthermore, the PdH nanozymes within the Mag‐gel mitigate the harsh inflammatory microenvironment, favoring cell survival and disc regeneration. This study presents a remote and multi‐staged strategy for chronologically regulating endogenous stem cell fate, supporting disc regeneration without invasive procedures.

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Macrophage membrane-functionalized manganese dioxide nanomedicine for synergistic treatment of atherosclerosis by mitigating inflammatory storms and promoting cholesterol efflux

Chen,

Zhang,

Tang

et al. 2024

J Nanobiotechnol

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Atherosclerosis (AS) poses a significant threat to human life and health. However, conventional antiatherogenic medications exhibit insufficient targeting precision and restricted therapeutic effectiveness. Moreover, during the progression of AS, macrophages undergo polarization toward the proinflammatory M1 phenotype and generate reactive oxygen species (ROS) to accelerate the occurrence of inflammatory storms, and ingest excess lipids to form foam cells by inhibiting cholesterol efflux. In our study, we developed a macrophage membrane-functionalized hollow mesoporous manganese dioxide nanomedicine (Col@HMnO 2 -MM). This nanomedicine has the ability to evade immune cell phagocytosis, enables prolonged circulation within the body, targets the inflammatory site of AS for effective drug release, and alleviates the inflammatory storm at the AS site by eliminating ROS. Furthermore, Col@HMnO 2 -MM has the ability to generate oxygen autonomously by breaking down surplus hydrogen peroxide generated at the inflammatory AS site, thereby reducing the hypoxic microenvironment of the plaque by downregulating hypoxia-inducible factor (HIF-1α), which in turn enhances cholesterol efflux to inhibit foam cell formation. In an APOE −/− mouse model, Col@HMnO 2 -MM significantly reduced inflammatory factor levels, lipid storage, and plaque formation without significant long-term toxicity. In summary, this synergistic treatment significantly improved the effectiveness of nanomedicine and may offer a novel strategy for precise AS therapy. Supplementary Information The online version contains supplementary material available at 10.1186/s12951-024-02939-x.

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Stem cell niches functionalized strategies for organ regeneration and manufacturing

Cited by 4 publications

References 139 publications

Converging neural-centric and mechano-regulation in organoid modeling for system biology and medicine

Converging neural-centric and mechano-regulation in organoid modeling for system biology and medicine

A Novel Superparamagnetic‐Responsive Hydrogel Facilitates Disc Regeneration by Orchestrating Cell Recruitment, Proliferation, and Differentiation within Hostile Inflammatory Niche

Macrophage membrane-functionalized manganese dioxide nanomedicine for synergistic treatment of atherosclerosis by mitigating inflammatory storms and promoting cholesterol efflux

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