Stem cell proteomes: A profile of human mesenchymal stem cells derived from umbilical cord blood

Abstract

Multipotent mesenchymal stem cells (MSCs) derived from human umbilical cord blood (UCB) represent promising candidates for the development of future strategies in cellular therapy. To create a comprehensive protein expression profile for UCB-MSCs, one UCB unit from a full-term delivery was isolated from the unborn placenta, transferred into culture, and their whole-cell protein fraction was subjected to two-dimensional electrophoresis (2-DE). Unambiguous protein identification was achieved with peptide mass fingerprinting matrix-assisted laser desorption/ionization - time of flight - mass spectrometry (MALDI-TOF-MS), peptide sequencing (MALDI LIFT-TOF/TOF MS), as well as gel-matching with previously identified databases. In overall five replicate 2-DE runs, a total of 2037 +/- 437 protein spots were detected of which 205 were identified representing 145 different proteins and 60 isoforms or post-translational modifications. The identified proteins could be grouped into several functional categories, such as metabolism, folding, cytoskeleton, transcription, signal transduction, protein degradation, detoxification, vesicle/protein transport, cell cycle regulation, apoptosis, and calcium homeostasis. The acquired proteome map of nondifferentiated UCB-MSCs is a useful inventory which facilitates the identification of the normal proteomic pattern as well as its changes due to activated or suppressed pathways of cytosolic signal transduction which occur during proliferation, differentiation, or other experimental conditions.

Mass spectrometry based proteomic analysis of human stem cells: a brief review

“…While mesenchymal stem cells (MSCs) possess the desirable characteristics of stem cells, they are easy to obtain and culture, making these cells attractive candidates for tissue engineering approaches in mesenchymal tissue regeneration (Feldmann et al, 2005). Their main resource is the bone marrow (BM).…”

“…Human umbilical cord blood (UCB) is an alternative source of hMSCs that is relatively free of such disadvantages. Feldmann et al (2005) compared the difference between BM-MSCs and UCB-MSCs. Immunological phenotype determined by surface proteins of UCB-MSCs was analyzed using FACS flow cytometry.…”

“…The metabolic protein group encompassed numerous biochemical pathways such as Krebs cycle, amino acid metabolism, cellular housekeeping, and protein biosynthesis. Feldmann et al (2005) identified the presence of protein vimentin in differentiating stem cells, which is normally absent in undifferentiated forms. Vimentin is the most omnipresent intermediate filament protein and is one of the first proteins to be expressed during the differentiation of the cell.…”

“…The protein prohibitin has been known for key functions in progenitor cells; two prohibitin proteins, Phb1p and Phb2p (BAP37), are involved in various functions such as cell cycle regulation, mitosis and proliferation, apoptosis, assembly of mitochondrial respiratory chain enzymes, and aging. Since prohibitins participate in G1/S phase regulation in cell cycle and interact with the retinoblastoma protein (pRB), prohibitins are considered as latent tumor suppressors (Feldmann et al, 2005).…”

Protein Expression Profiles during Osteogenic Differentiation of Mesenchymal Stem Cells Derived from Human Umbilical Cord Blood

“…Mesenchymal stem cells (MSCs) have the potential to differentiate along multiple lineages and can be expanded in vitro, making them highly attractive candidates for cell therapy and tissue engineering applications (Feldmann et al 2005). Laboratory methods for inducing MSC differentiation into osteoblasts are well established (Bieback et al 2004;Yang et al 2004), and a number of studies have exploited osteogenic mechanisms, utilizing differentiated osteoblasts as a bone-defect replacement strategy.…”