Stem Cell Therapies for Wound Healing

Grada, Ayman; Falanga, Vincent

doi:10.1007/15695_2017_100

Cited by 4 publications

(6 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These proteins play an important role in enhancing reepithelization, angiogenesis, and wound closure (Clark et al, 1997;Solberg et al, 2001;Blasi and Carmeliet, 2002;Li et al, 2003;Werner and Grose, 2003;Barrientos et al, 2008;D'Alessio et al, 2008;McDowall et al, 2008;de Kretser et al, 2012;Pakyari et al, 2013;Rao et al, 2013;Liu et al, 2016;Grada and Falanga, 2018;Cooper et al, 2018;Ferreira and Gomes, 2018;Mahmood et al, 2018;Park et al, 2018;Ahangar et al, 2020;Huang et al, 2020;Johnson et al, 2020;Ham et al, 2021). Interestingly, Insulin and VEGF concentrations were higher in hypoxic CM than in normoxic CM.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, Insulin and VEGF concentrations were higher in hypoxic CM than in normoxic CM. It is known that Insulin and VEGF expressions are up-regulated in hypoxia and stimulate angiogenesis by promoting endothelial cell survival, proliferation, migration, and remodeling (Werner and Grose, 2003;Das et al, 2010;Escudero et al, 2017;Ferreira and Gomes, 2018;Grada and Falanga, 2018;Ahangar et al, 2020;Huang et al, 2020). Previous studies also demonstrated that hypoxic CM enhanced wound closure compared with normoxic CM and found that hypoxic CM contains higher amounts of proteins, e.g., bFGF, VEGF, IL-6, and TGFβ-1 (Chen et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…The major role of FGF-7 (Werner and Grose, 2003;Barrientos et al, 2008;Grada and Falanga, 2018;Huang et al, 2020), Follistatin (McDowall et al, 2008de Kretser et al, 2012;Ham et al, 2021), IL-6 (Werner and Grose, 2003;Ferreira and Gomes, 2018;Johnson et al, 2020), TGFβ-1 (Clark et al, 1997;Barrientos et al, 2008;Pakyari et al, 2013;Liu et al, 2016;Ahangar et al, 2020), and uPAR (Solberg et al, 2001;Blasi and Carmeliet, 2002;Li et al, 2003;D'Alessio et al, 2008;Rao et al, 2013;Cooper et al, 2018;Mahmood et al, 2018) includes promoting re-epithelialization, extracellular matrix production and wound closure. ANG-1 (Werner and Grose, 2003;Ferreira and Gomes, 2018;Grada and Falanga, 2018;Ahangar et al, 2020), HGF (Werner and Grose, 2003;Ferreira and Gomes, 2018;Grada and Falanga, 2018;Park et al, 2018;Huang et al, 2020), Insulin (Liu et al, 2009;Escudero et al, 2017), and VEGF (Werner and Grose, 2003;Ferreira and Gomes, 2018;Grada and Falanga, 2018;Ahangar et al, 2020;Huang et al, 2020) promote angiogenesis. Based on the proteomics study, the composition and concentration of nine r...…”

Section: Hpsc-derived Conditioned Medium Analysismentioning

confidence: 99%

See 2 more Smart Citations

Identification and characterization of stem cell secretome-based recombinant proteins for wound healing applications

Kim

Green

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Stem cells have been introduced as a promising therapy for acute and chronic wounds, including burn injuries. The effects of stem cell-based wound therapies are believed to result from the secreted bioactive molecules produced by stem cells. Therefore, treatments using stem cell-derived conditioned medium (CM) (referred to as secretome) have been proposed as an alternative option for wound care. However, safety and regulatory concerns exist due to the uncharacterized biochemical content and variability across different batches of CM samples. This study presents an alternative treatment strategy to mitigate these concerns by using fully characterized recombinant proteins identified by the CM analysis to promote pro-regenerative healing. This study analyzed the secretome profile generated from human placental stem cell (hPSC) cultures and identified nine predominantly expressed proteins (ANG-1, FGF-7, Follistatin, HGF, IL-6, Insulin, TGFβ-1, uPAR, and VEGF) that are known to contribute to wound healing and angiogenesis. These proteins, referred to as s (CMFs), were used in combination to test the effects on human dermal fibroblasts (HDFs). Our results showed that CMF treatment increased the HDF growth and accelerated cell migration and wound closure, similar to stem cell and CM treatments. In addition, the CMF treatment promoted angiogenesis by enhancing new vessel formation. These findings suggest that the defined CMF identified by the CM proteomic analysis could be an effective therapeutic solution for wound healing applications. Our strategy eliminates the regulatory concerns present with stem cell-derived secretomes and could be developed as an off-the-shelf product for immediate wound care and accelerating healing.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Hpsc-derived Conditioned Medium Analysismentioning

confidence: 99%

See 1 more Smart Citation

Identification and characterization of stem cell secretome-based recombinant proteins for wound healing applications

Kim

Green

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Endothelial cells, fibroblasts and stem cells are triggered to promote tissue repair by stimulating proliferation, migration and differentiation of the most important cell types (keratinocytes and fibroblasts), increasing angiogenesis and restraining leukocyte transmigration [45]. Moreover, MSCs have the capability to release immunosuppressive factors that suppress immune cells proliferation, and this anti-inflammatory effect helps to reduce the scar and optimize tissue repair [28,46]. The skin regeneration varies upon the wound's microenvironment, which depends itself from systemic factors (like systemic diseases or inflammation) [7].…”

Section: Cell-based Therapies In Wound Healingmentioning

confidence: 99%

The Application of Mesenchymal Stem Cells on Wound Repair and Regeneration

et al. 2021

View full text Add to dashboard Cite

In the past decades, regenerative medicine applied on skin lesions has been a field of constant improvement for both human and veterinary medicine. The process of healing cutaneous wound injuries implicates a well-organized cascade of molecular and biological processes. However, sometimes the normal process fails and can result in a chronic lesion. In addition, wounds are considered an increasing clinical impairment, due to the progressive ageing of the population, as well as the prevalence of concomitant diseases, such as diabetes and obesity, that represent risk-aggravating factors for the development of chronic skin lesions. Stem cells’ regenerative potential has been recognized worldwide, including towards skin lesion repair, Tissue engineering techniques have long been successfully associated with stem cell therapies, namely the application of three-dimensional (3D) bioprinted scaffolds. With this review, we intend to explore several stem cell sources with promising aptitude towards skin regeneration, as well as different techniques used to deliver those cells and provide a supporting extracellular matrix environment, with effective outcomes. Furthermore, different studies are discussed, both in vitro and in vivo, in terms of their relevance in the skin regeneration field.

show abstract

“…Besides, MSCs have the capability of releasing immunosuppressive factors that suppress immune cells proliferation, this anti-inflammatory effect helps to reduce the scar and optimize tissue repairment. [31], [55] The skin regeneration varies upon the wound's microenvironment, which depends itself from systemic factors (like systemic diseases or inflammation). [7] Stem cell secretome, such as conditioned media or purified exosomes, is another viable therapeutic option.…”

Section: Cell-based Therapies In Wound-healingmentioning

confidence: 99%

The Application of Mesenchymal Stem Cells on Wound Repair and Regeneration

Lopes¹,

Sousa²,

Alvites³

et al. 2021

Preprint

View full text Add to dashboard Cite

In the past decades, regenerative medicine applied on skin lesions has been a field of constant improvement for both human and veterinary medicine. The process of healing cutaneous wound injuries implicates a well-organized cascade of molecular and biological processes. However, sometimes the normal process fails and can result in a chronic lesion. In addition, wounds are considered an increasing clinical impairment, due to the progressive ageing of the population, as well as the prevalence of concomitant diseases, such as diabetes and obesity, that represent risk aggravating factors for the development of chronic skin lesions. Stem cells regenerative potential has been recognized worldwide, including towards skin lesion repair, Tissue engineering techniques have long been successfully associated with stem cell therapies, namely the application of 3D bioprinted scaffolds. With this review we intend to explore several stem cell sources with promising aptitude towards skin regeneration, as well as different techniques used to deliver those cells and provide a supporting extracellular matrix environment, with effective outcomes. Furthermore, different studies are discussed, both in vitro and in vivo, towards their relevance in the skin regeneration field.

show abstract

Stem Cell Therapies for Wound Healing

Cited by 4 publications

References 87 publications

Identification and characterization of stem cell secretome-based recombinant proteins for wound healing applications

Identification and characterization of stem cell secretome-based recombinant proteins for wound healing applications

The Application of Mesenchymal Stem Cells on Wound Repair and Regeneration

The Application of Mesenchymal Stem Cells on Wound Repair and Regeneration

Contact Info

Product

Resources

About