Stem cells and growth factors-based delivery approaches for chronic wound repair and regeneration: A promise to heal from within

Sharma, Priyank; Kumar, Arun; Dey, Asmita Deka; Behl, Tapan; Chadha, Swati

doi:10.1016/j.lfs.2020.118932

Cited by 41 publications

(26 citation statements)

References 202 publications

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“…Nevertheless, nanofibers are presently being studied and improved for their application in tissue regeneration, both in wound healing and beauty treatment. In the former case, nanofibers are exploited to deliver drugs or other biological components (e.g., growth factors [118] and stem cells [119] to the wound site; in the latter, facial masks made of nanofibers are actually used for the release of collagen and vitamins on human skin [41]. A schematic example of a nanofiber-based wound dressing could be found in Figure 2.…”

Section: Nanofibers and Scaffoldsmentioning

confidence: 99%

“…A schematic example of a nanofiber-based wound dressing could be found in Figure 2. Among the many examples of drug-loaded nanofibers, a cerium-doped bioactive glass in a chitosan/polyethylene oxide nanofiber showed remarkably high antibacterial action against both Gram-negative and Gram-positive bacterial strains [119]. Heparin has high affinity for many growth factors that are crucial biological mediators in the wound healing mechanism.…”

Section: Nanofibers and Scaffoldsmentioning

confidence: 99%

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Nanomaterials in Skin Regeneration and Rejuvenation

Bellu

Medici

Coradduzza

et al. 2021

IJMS

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Skin is the external part of the human body; thus, it is exposed to outer stimuli leading to injuries and damage, due to being the tissue mostly affected by wounds and aging that compromise its protective function. The recent extension of the average lifespan raises the interest in products capable of counteracting skin related health conditions. However, the skin barrier is not easy to permeate and could be influenced by different factors. In the last decades an innovative pharmacotherapeutic approach has been possible thanks to the advent of nanomedicine. Nanodevices can represent an appropriate formulation to enhance the passive penetration, modulate drug solubility and increase the thermodynamic activity of drugs. Here, we summarize the recent nanotechnological approaches to maintain and replace skin homeostasis, with particular attention to nanomaterials applications on wound healing, regeneration and rejuvenation of skin tissue. The different nanomaterials as nanofibers, hydrogels, nanosuspensions, and nanoparticles are described and in particular we highlight their main chemical features that are useful in drug delivery and tissue regeneration.

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Section: Nanofibers and Scaffoldsmentioning

confidence: 99%

Section: Nanofibers and Scaffoldsmentioning

confidence: 99%

Nanomaterials in Skin Regeneration and Rejuvenation

Bellu

Medici

Coradduzza

et al. 2021

IJMS

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“…Skin wound healing is a complex biological process involving a huge variety of cells producing matrix modeling proteins, proteinases, cytokines such as chemoattractants, growth factors, and angiogenic factors [ 1 ]. Chronic wounds, such as leg ulcers associated with sickle cell disease, pressure ulcers, and vascular ulcers, occur as consequence of a prolonged inflammatory phase during the healing process [ 2 , 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…Current treatment for chronic wounds involves preventing wound infections, debriding the tissue and selecting appropriate dressings to maintain favorable wound-healing environment. Many wound dressings have been developed to try to both protect the healing wound from infection and also to help promote the wound healing process itself [ 1 , 6 , 7 ]. As a matter of fact, the approach of regenerative medicine using stem cells has emerged to provide new therapeutic options in the domain of wound healing [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…The most common stem cells used in skin regeneration and wound healing are adult stem cells owing to containing significant proliferative capacity and having the ability to differentiate into limited tissue-specific cells. Among the different types of adult stem cells, keratinocytes (KER) or mesenchymal stem cells (MSCs) have gained considerable attention as suitable candidates to enhance tissue regeneration [ 1 , 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Clinical Grade Human Pluripotent Stem Cell-Derived Engineered Skin Substitutes Promote Keratinocytes Wound Closure In Vitro

Domingues

Darle

Masson

et al. 2022

Cells

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Chronic wounds, such as leg ulcers associated with sickle cell disease, occur as a consequence of a prolonged inflammatory phase during the healing process. They are extremely hard to heal and persist as a significant health care problem due to the absence of effective treatment and the uprising number of patients. Indeed, there is a critical need to develop novel cell- and tissue-based therapies to treat these chronic wounds. Development in skin engineering leads to a small catalogue of available substitutes manufactured in Good Manufacturing Practices compliant (GMPc) conditions. Those substitutes are produced using primary cells that could limit their use due to restricted sourcing. Here, we propose GMPc protocols to produce functional populations of keratinocytes and fibroblasts derived from pluripotent stem cells to reconstruct the associated dermo-epidermal substitute with plasma-based fibrin matrix. In addition, this manufactured composite skin is biologically active and enhances in vitro wounding of keratinocytes. The proposed composite skin opens new perspectives for skin replacement using allogeneic substitute.

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Hydrogel foam dressings with angiogenic and immunomodulatory factors from mesenchymal stem cells

Lan,

Fletcher,

Bender

et al. 2024

J Biomedical Materials Res

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Stem cell therapy and skin substitutes address the stalled healing of chronic wounds in order to promote wound closure; however, the high cost and regulatory hurdles of these treatments limit patient access. A low‐cost method to induce bioactive healing has the potential to substantially improve patient care and prevent wound‐induced limb loss. A previous study reported that bioactive factors derived from apoptotic‐like mesenchymal stem cells (MSCs) demonstrated anti‐inflammatory and proangiogenic effects and improved ischemic muscle regeneration. In this work, these MSC‐derived bioactive factors were loaded into a hydrogel foam to harness immunomodulatory and angiogenic properties from MSC components to facilitate chronic wound healing without the high cost and translational challenges of cell therapies. After incorporation of bioactive factors, the hydrogel foam retained high absorbency, moisture retention, and target water vapor transmission rate. High loading efficiency was confirmed and release studies indicated that over 90% of loaded factors were released within 24 h. Ethylene oxide sterilization and 4‐week storage did not affect the bioactive factor release profile or physical properties of the hydrogel foam dressing. Bioactivity retention of the released factors was also confirmed for as‐sterilized, 4°C‐stored, and −20°C‐stored bioactive hydrogel foams as determined by relevant gene expression levels in treated pro‐inflammatory (M1) macrophages. These results support the use of the bioactive dressings as an off‐the‐shelf product. Overall, this work reports a new method to achieve a first‐line wound dressing with the potential to reduce persistent inflammation and promote angiogenesis in chronic wounds.

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Stem cells and growth factors-based delivery approaches for chronic wound repair and regeneration: A promise to heal from within

Cited by 41 publications

References 202 publications

Nanomaterials in Skin Regeneration and Rejuvenation

Nanomaterials in Skin Regeneration and Rejuvenation

Clinical Grade Human Pluripotent Stem Cell-Derived Engineered Skin Substitutes Promote Keratinocytes Wound Closure In Vitro

Hydrogel foam dressings with angiogenic and immunomodulatory factors from mesenchymal stem cells

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