Stem Cells and Hydrogels for Liver Tissue Engineering: Synergistic Cure for Liver Regeneration

Nadi, Akram; Moradi, Lida; Ai, Jafar; Asadpour, Shiva

doi:10.1007/s12015-020-10060-3

Cited by 19 publications

(9 citation statements)

References 118 publications

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“…The current study assumes that the mixture of gelatin and silk with AA and PCL provides a better biomaterial with appropriate mechanical strength, flexibility, water absorption, biocompatibility, and biological interactions. Among all cell sources, mesenchymal stem cells (MSCs), because of their capability of differentiation to different mesenchymal lineages, anti-inflammatory, and immunomodulatory features [28][29][30][31][32][33][34] are of particular interest. Adipose-derived mesenchymal stem cells (ASCs) obtained from the infrapatellar fat pad of the knee can differentiate into chondrocytes and produce proteoglycans and type II collagen.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Macroporous Polycaprolactone/Silk Fibroin/Gelatin/Ascorbic Acid Composite Scaffolds and In Vivo Results in a Rabbit Model for Meniscus Cartilage Repair

et al. 2021

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Objective Meniscus injuries in the inner avascular zone have weak intrinsic self-healing capacity and often progress to osteoarthritis. This study focused on evaluating the effects of polycaprolactone/silk fibroin/gelatin/ascorbic acid (PCL/SF/Gel/AA) composite scaffolds seeded with adipose-derived mesenchymal stem cells (ASCs), in the meniscus repair. Design To this end, composite scaffolds were cross-linked using N-hydroxysuccinimide and 1-ethyl-3-(3-dimethyl-aminopropyl)-1-carbodiimide hydrochloride. Scaffolds were then characterized by scanning electron microscope, mechanical tests, total antioxidant capacity, swelling, and toxicity tests. Results The PCL/SF/Gel/AA scaffolds exhibited suitable mechanical properties. Furthermore, vitamin C rendered them the highest antioxidant capacity. The PCL/SF/Gel/AA scaffolds also showed good biocompatibility and proliferation for chondrocytes. Moreover, the PCL/SF/Gel/AA scaffold seeded with allogeneic ASCs was engrafted in New Zealand rabbits who underwent unilateral punch defect in the medial meniscus of the right knee. After 2 months postimplantation, macroscopic and histologic studies for new meniscus cartilage were performed. Conclusions Our results indicated that the PCL/SF/Gel/AA composite scaffolds seeded with allogeneic ASCs could successfully improve meniscus healing in damaged rabbits.

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Section: Introductionmentioning

confidence: 99%

Characterization of Macroporous Polycaprolactone/Silk Fibroin/Gelatin/Ascorbic Acid Composite Scaffolds and In Vivo Results in a Rabbit Model for Meniscus Cartilage Repair

et al. 2021

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“…There are different treatment modalities and ongoing research studies concentrating on new treatment options (Table 1) [21,28,37,39,47,[52][53][54][55][56][57][58][61][62][63][64][65][66][67][68][69][70][71][72][73][74][75][76][77][78][79]. One of the most important facts in treatment are to hold plasma homocysteine as close as to the normal range [1-8, 21, 23, 24, 26].…”

Section: Current and Novel Therapy Optionsmentioning

confidence: 99%

“…Different studies were performed in the last years to optimize liver engineered organoids and to make it transplantable in any way into patients with different liver diseases. This could also be an interesting treatment option of HCU patients in the future [75][76][77][78][79].…”

Section: Liver-directed Therapy (Three Dimensional (3d) Bioprinting/l...mentioning

confidence: 99%

“…A very interesting aspect curing the disease is based on liver-directed therapy like 3-D-bioprinting and liver bioengineering. Engineering of liver organoids in vitro and later implantation of these tissues into the HCU patient could have a curing aspect in patients with HCU to restore the function of cystathionine beta synthase without completely removing the whole organ in cases of liver transplantation [75,76,78,[80][81][82]. Liver transplantation therefore should only be performed in serious and individual cases, where no other treatment option leads to success.…”

Section: Gene Therapymentioning

confidence: 99%

“…Liver transplantation therefore should only be performed in serious and individual cases, where no other treatment option leads to success. Another aspect is the transplantation of liver progenitor cells from bone marrow for liver regeneration and restoring the function of the disturbed enzyme [71][72][73][74][75][76][77][78][79]. Cure of patients with HCU could also be achieved by onestage gene therapy maneuvers in the future.…”

Section: Gene Therapymentioning

confidence: 99%

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Current and Novel Therapeutical Approaches of Classical Homocystinuria in Childhood With Special Focus on Enzyme Replacement Therapy, Liver-Directed Therapy and Gene Therapy

Bittmann¹,

Villalon²,

Moschüring-Alieva³

et al. 2023

J Clin Med Res

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Classical homocystinuria is a hereditary defect of the enzyme cystathionine beta synthase, which is produced in the liver. If this enzyme fails, the synthesis pathway of cysteine from methionine is interrupted, leading to the accumulation of homocysteine in the blood plasma and homocysteine in the urine. After birth, the children are unremarkable except for the characteristic laboratory findings. Symptoms rarely appear before the second year of life. The most common symptom is a prolapse of the crystalline lens. This finding is seen in 70% of untreated 10-year-old affected individuals. As the earliest symptom, psychomotor retardation occurs in the majority of patients already during the first two years of life. Limiting factors in terms of life expectancy are thromboembolism, peripheral arterial disease, myocardial infarction, and stroke. These symptoms are due to the damage to the vessels caused by the elevated amino acid levels. About 30% suffer a thromboembolic event by the age of 20, about half by the age of 30. This review focus on present and new therapeutical approaches like the role of enzyme replacement with presentation of different novel targets in research like pegtibatinase, pegtarviliase, CDX-6512, erymethionase, chaperones, proteasome inhibitors and probiotic treatment with SYNB 1353. Furthermore, we analyze the role of liver-directed therapy with three dimensional (3D) bioprinting, liver bioengineering of liver organoids in vitro and liver transplantation. The role of different gene therapy options to treat and cure this extremely rare disease in childhood will be discussed.

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Hybrid Bioactive Hydrogel Promotes Liver Regeneration through the Activation of Kupffer Cells and ECM Remodeling After Partial Hepatectomy

Pan,

Zheng,

Zhang

et al. 2024

Adv Healthcare Materials

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Partial hepatectomy is an essential surgical technique used to treat advanced liver diseases such as liver tumors, as well as for performing liver transplants from living donors.However, postoperative complications such as bleeding, abdominal adhesions, wound infections, and accelerated liver regeneration pose significant challenges and increase morbidity and mortality rates.We have developed a self‐repairing mixed hydrogel (O5H2/Cu2+/SCCK) containing bioactive cell factors (SCCK) derived from Human Umbilical Cord Mesenchymal Stem Cells (HUMSCs) treated with the traditional Chinese remedy Tanshinone IIA (TSA).This hydrogel, in conjunction with O5H2, demonstrated remarkable effects on Kupffer cell activation and extracellular matrix (ECM) remodeling.This led to the secretion of critical growth factors promoting enhanced proliferation of hepatocytes and endothelial cells, thereby facilitating liver regeneration and repair after partial hepatectomy.Furthermore, the hydrogel, featuring macrophage‐regulating properties, effectively mitigated inflammation and oxidative stress damage in the incision area, creating an optimal environment for postoperative liver regeneration.The injectability and strong adhesion of the hydrogel enables rapid hemostasis at the incision site, while its physical barrier function prevents postoperative abdominal adhesions.Furthermore, the hydrogel's incorporation of Cu2+ provided comprehensive antibacterial effects, protecting against a wide range of bacteria types and reducing the chances of infections after surgery. This combined method shows promise in improving tissue healing and functional results after partial hepatectomy and living donor liver transplantation, ultimately enhancing patients' quality of life.This article is protected by copyright. All rights reserved

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Stem Cells and Hydrogels for Liver Tissue Engineering: Synergistic Cure for Liver Regeneration

Cited by 19 publications

References 118 publications

Characterization of Macroporous Polycaprolactone/Silk Fibroin/Gelatin/Ascorbic Acid Composite Scaffolds and In Vivo Results in a Rabbit Model for Meniscus Cartilage Repair

Characterization of Macroporous Polycaprolactone/Silk Fibroin/Gelatin/Ascorbic Acid Composite Scaffolds and In Vivo Results in a Rabbit Model for Meniscus Cartilage Repair

Current and Novel Therapeutical Approaches of Classical Homocystinuria in Childhood With Special Focus on Enzyme Replacement Therapy, Liver-Directed Therapy and Gene Therapy

Hybrid Bioactive Hydrogel Promotes Liver Regeneration through the Activation of Kupffer Cells and ECM Remodeling After Partial Hepatectomy

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