Stem Cells are Mobilized from the Bone Marrow into the Peripheral Circulation in Response to Retinal Pigment Epithelium Damage—A Pathophysiological Attempt to Induce Endogenous Regeneration

Machalińska, Anna; Kłos, Patrycja; Baumert, Bartłomiej; Baśkiewicz, Magdalena; Kawa, Miłosz; Rudnicki, Michał; Lubiński, Wojciech; Wiszniewska, Barbara; Karczewicz, Danuta; Machaliński, Bogusław

doi:10.3109/02713683.2011.576796

Cited by 21 publications

(20 citation statements)

References 31 publications

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Section: Sources Of Endogenous Stem Cells/progenitor Cellsmentioning

confidence: 99%

“…In mice, it has been shown that endogenous BMCs can migrate to the subretinal space in the damaged retina, presumably to initiate or participate in neural repair [52][53][54] . However, no evidence has been suggested that they can transdifferentiate into cells with anatomical or functional characteristics of retinal neurons [53][54] . To date, Müller cells are the best characterized mammalian cell type that shows retinal progenitor cell properties and generates new retinal neurons after injury in adult mammals [55][56][57] .…”

Section: Sources Of Endogenous Stem Cells/progenitor Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Mobilizing Endogenous Stem Cells for Retinal Repair

Yu¹,

Talib²,

Vu³

et al. 2016

Translating Regenerative Medicine to the Clinic

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Section: Sources Of Endogenous Stem Cells/progenitor Cellsmentioning

confidence: 99%

Section: Sources Of Endogenous Stem Cells/progenitor Cellsmentioning

confidence: 99%

Mobilizing Endogenous Stem Cells for Retinal Repair

Yu¹,

Talib²,

Vu³

et al. 2016

Translating Regenerative Medicine to the Clinic

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“…An alternative approach is the employment of the endogenous population of BMSCs by mobilizing it out of the bone marrow (BM) into the periphery and directing its migration towards the site of injury (21). Therefore, we have investigated the potential of mobilized endogenous…”

Section: Introductionmentioning

confidence: 99%

CXCL12/SDF-1-Dependent Retinal Migration of Endogenous Bone Marrow-Derived Stem Cells Improves Visual Function after Pharmacologically Induced Retinal Degeneration

Enzmann

Lecaude

Kruschinski

et al. 2016

Stem Cell Rev and Rep

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Mobilized bone marrow-derived stem cells (BMSC) have been discussed as an alternative strategy for endogenous repair. Thereby, different approaches for BMSC mobilization have been pursued. Herein, the role of a newly discovered oligonucleotide for retinal homing and regeneration capability of BMSCs was investigated in the sodium iodate (NaIO 3 ) model of retinal degeneration. Mobilization was achieved in GFP-chimera with NOX-A12, a CXC-motif chemokine ligand 12 (CXCL12)/stromal cell-derived factor 1 (SDF-1)-neutralizing L-aptamer.

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“…Furthermore, BMSCs can be triggered to commit to and progress along the RPE differentiation pathway in culture [12] . BMSCs also home to the subretinal space if systemically transferred or endogenously mobilized where they express RPE lineage markers [13][14][15] .…”

Section: Research Articlementioning

confidence: 99%

Bone marrow-derived stem cells differentiate into retinal pigment epithelium-like cells in vitro but are not able to repair retinal degeneration in vivo

Lecaude

Wolf

Abdillahi

et al. 2015

Stem Cell Transl Investig

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The bone marrow (BM) is home to different stem/progenitor populations, including tissue-committed stem cells. In this context, we have cocultured BM-derived stem cells (BMSC) in order to investigate their differentiation capacity towards the retinal pigment epithelial (RPE) lineage in vitro. Furthermore, pre-differentiated BMSC were transplanted into the pharmacologically damaged subretinal space to determine their rescue ability in vivo. BM was harvested from the tibias and femurs of adult GFP + C57BL/6 mice. Differentiated hematopoietic cells were removed by lineage depletion, and CD45 -BMSCs were separated by magnetic activated cell sorting (MACS). To induce differentiation, the cells were then cocultured with murine RPE for 10 days, and retinal markers were assessed using immunohistochemistry (IHC). To induce retinal degeneration, mice were treated with sodium iodate (NaIO 3 ). Seven days later, approx. 60,000 pre-differentiated GFP + BMSC, sorted by FACS, were transplanted subretinally. Optical coherence tomography (OCT) was used to follow the transplants and to quantify the retinal thickness over time. Visual acuity was measured concurrently using the optokinetic reflex (OKR). Finally, IHC was performed to investigate the expression of retina-specific markers in the transplants. CD45-BMSC adopted an RPE-like elongated morphology and showed expression of the RPE markers RPE65 and bestrophin after coculture. After transplantation of CD45 -BMSC, visual acuity increased in individual animals compared to the contralateral control eye, but did not reach baseline levels. Additionally, no significant increase in retinal thickness in the transplanted eye was found. However, the cells were detectable in the subretinal space for up to 28 days and expressed the RPE markers RPE65 and bestrophin. In summary, the BMSC differentiated into RPE-like cells but were not able to restore visual function or rescue retinal morphology after subretinal transplantation.Keywords: retina; degeneration; sodium iodate; transplantation; differentiation; bone marrow-derived stem cells To cite this article: Stéphanie Lecaudé, et al. Bone marrow-derived stem cells differentiate into retinal pigment epithelium-like cells in vitro but are not able to repair retinal degeneration in vivo.

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Stem Cells are Mobilized from the Bone Marrow into the Peripheral Circulation in Response to Retinal Pigment Epithelium Damage—A Pathophysiological Attempt to Induce Endogenous Regeneration

Cited by 21 publications

References 31 publications

Mobilizing Endogenous Stem Cells for Retinal Repair

Mobilizing Endogenous Stem Cells for Retinal Repair

CXCL12/SDF-1-Dependent Retinal Migration of Endogenous Bone Marrow-Derived Stem Cells Improves Visual Function after Pharmacologically Induced Retinal Degeneration

Bone marrow-derived stem cells differentiate into retinal pigment epithelium-like cells in vitro but are not able to repair retinal degeneration in vivo

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