Stem cells in intervertebral disc regeneration–more talk than action?

Kraus, Petra; Samanta, Ankita; Lufkin, Sina; Lufkin, Thomas

doi:10.32604/biocell.2022.018432

Cited by 4 publications

(5 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regenerative medicine employs cell-based therapies amidst other strategies ( Kraus et al, 2021 ). Stem or progenitor cells are a valuable but sparse resource and typically require expansion in culture for quality control and enrichment purposes prior to therapeutic applications.…”

Section: Discussionmentioning

confidence: 99%

“…Several transcripts we found higher in FAT over IVD cells. A benefit of adult stromal cells is their multipotent over pluripotent differentiation potential, a restriction rendering them less tumorigenic than induced pluripotent or embryonic stem cells, however, potentially hampering their plasticity in replacing ailing cells ( Pittenger et al, 1999 ; Kraus et al, 2021 ). Oct4 is known as a stemness and reprogramming marker but also has a role in lineage specification ( Zeineddine et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

“…IVDD cell therapies long engaged in the idea of using stem cells to replace ailing NP cells, increase ECM production, and restore IVD function ( Wuertz et al, 2008 ; Sivakamasundari and Lufkin, 2013 ; Pennicooke et al, 2016 ; Kraus and Lufkin, 2017 ; Sakai and Schol, 2017 ; Xia et al, 2019 ; Wang et al, 2021b ). Mesenchymal stem or stromal cells (MSCs) have attracted attention given their multi-over pluripotency, with the benefit of reduced tumorigenicity ( Kraus et al, 2021 ). Among several stromal cell sources in the context of IVDD, the AF tissue would be more easily accessible than NP tissue, whereas subcutaneous adipose MSCs are an established source ( Rodriguez et al, 2004 ; Rodriguez et al, 2005 ) ( Table 1 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Glis1 and oxaloacetate in nucleus pulposus stromal cell somatic reprogramming and survival

Lufkin

Samanta

Baker

et al. 2022

Front. Mol. Biosci.

View full text Add to dashboard Cite

Regenerative medicine aims to repair degenerate tissue through cell refurbishment with minimally invasive procedures. Adipose tissue (FAT)-derived stem or stromal cells are a convenient autologous choice for many regenerative cell therapy approaches. The intervertebral disc (IVD) is a suitable target. Comprised of an inner nucleus pulposus (NP) and an outer annulus fibrosus (AF), the degeneration of the IVD through trauma or aging presents a substantial socio-economic burden worldwide. The avascular nature of the mature NP forces cells to reside in a unique environment with increased lactate levels, conditions that pose a challenge to cell-based therapies. We assessed adipose and IVD tissue-derived stromal cells through in vitro transcriptome analysis in 2D and 3D culture and suggested that the transcription factor Glis1 and metabolite oxaloacetic acid (OAA) could provide NP cells with survival tools for the harsh niche conditions in the IVD.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Glis1 and oxaloacetate in nucleus pulposus stromal cell somatic reprogramming and survival

Lufkin

Samanta

Baker

et al. 2022

Front. Mol. Biosci.

View full text Add to dashboard Cite

show abstract

“…IVDD can also secrete Stromal cell-derived factor-1a (SDF-1a) to induce the chemotaxis of NPSCs to the NPs center for in situ regeneration (27). IVD regeneration is mainly achieved by inducing the differentiation of MSCs into NPCs or AFCs (28)(29)(30). Although MSCs have achieved good results in bone and cartilage regeneration, similar effects cannot currently be exerted in IVDD, even if the MSCs were delivered to IVDs, due to the lack of vascular systems in the discs (31).…”

Section: Introductionmentioning

confidence: 99%

Biomaterials delivery strategies to repair degenerated intervertebral discs by regulating the inflammatory microenvironment

et al. 2023

View full text Add to dashboard Cite

Intervertebral disc degeneration (IVDD) is one of the leading causes of lower back pain. Although IVDD cannot directly cause death, it can cause pain, psychological burdens, and economic burdens to patients. Current conservative treatments for IVDD can relieve pain but cannot reverse the disease. Patients who cannot tolerate pain usually resort to a strategy of surgical resection of the degenerated disc. However, the surgical removal of IVDD can affect the stability of adjacent discs. Furthermore, the probability of the reherniation of the intervertebral disc (IVD) after surgery is as high as 21.2%. Strategies based on tissue engineering to deliver stem cells for the regeneration of nucleus purposes (NP) and annulus fibrosus (AF) have been extensively studied. The developed biomaterials not only locally withstand the pressure of the IVD but also lay the foundation for the survival of stem cells. However, the structure of IVDs does not provide sufficient nutrients for delivered stem cells. The role of immune mechanisms in IVDD has recently become clear. In IVDD, the IVD that was originally in immune privilege prevents the attack of immune cells (mainly effector T cells and macrophages) and aggravates the disease. Immune regulatory and inflammatory factors released by effector T cells, macrophages, and the IVD further aggravate IVDD. Reversing IVDD by regulating the inflammatory microenvironment is a potential approach for the treatment of the disease. However, the biological factors modulating the inflammatory microenvironment easily degrade in vivo. It makes it possible for different biomaterials to modulate the inflammatory microenvironment to repair IVDD. In this review, we have discussed the structures of IVDs and the immune mechanisms underlying IVDD. We have described the immune mechanisms elicited by different biological factors, including tumor necrosis factors, interleukins, transforming growth factors, hypoxia-inducible factors, and reactive oxygen species in IVDs. Finally, we have discussed the biomaterials used to modulate the inflammatory microenvironment to repair IVDD and their development.

show abstract

“…Given that one of the key elements of the chain of IVD degeneration is a decrease in the number of cells and their metabolic activity, it can be assumed that cell therapy of this pathology should show a fairly high clinical efficacy [5]. In fact, the administration of mesenchymal stem cells (MSCs), which have high regenerative potential and the ability to differentiate in several directions, including chondrogenic, has already given promising results in this pathology [6]. In our previous studies, we have also obtained good outcomes: MSCs contributed to IVD restoration providing the most pronounced stimulatory effect on the fibrochondrocyte density in annulus fibrosus [7].…”

Section: Introductionmentioning

confidence: 99%

Effect of potentiated cryopreserved mesenchymal stem cells in intervertebral disc repair

Yukhta¹,

Volkova²,

Stepanyuk³

et al. 2022

Fiziol Zh

View full text Add to dashboard Cite

The aim of the study was to investigate the effect of cryopreserved mesenchymal stem cells (CrMSCs) from different tissue (bone marrow, adipose tissue, cartilage) potentiated with some growth and differentiation factors (TGFβ and bFGF) on intervertebral disc (IVD) cartilage repair using compression model of degenerative damage. In the group with self-healing histological changes in IVD cartilage tissue were pronounced. Administration of untreated CrMSCs (regardless of their origin) regenerated IVD structure up to a moderate degree on the 45th day. After therapy with TGFβ-potentiated cells, the histological structure of the disc remained similar to untreated CrMSC influence, which according to the integrated semi-quantitative scale corresponded to moderate degenerative changes. Whereas, the administration of bFGF-potentiated cells contributed to the restoration of the disc structure up to a mild degree of histological damages. In the latter case, there was an increase in cellularity of the fibrous ring, regeneration of cracks and gaps, and restoration of collagen febrile structure in annulus fibrosus on the 45th day after therapy. At the same time, the average cell density of fibrous rings increased by 1.35, 1.50, and 1.39 times and IVD height grew by 1.33, 1.36, and 1.37 times for bone marrow-, adipose- and cartilage-derived CrMSC of potentiated by bFGF respectively compared to untreated analog. Thus, administration of CrMSCs from all studied sources activates regenerative processes in degenerated intervertebral discs of rats, the rate of which increases using of bFGF-potentiated cells.

show abstract

Stem cells in intervertebral disc regeneration–more talk than action?

Cited by 4 publications

References 84 publications

Glis1 and oxaloacetate in nucleus pulposus stromal cell somatic reprogramming and survival

Glis1 and oxaloacetate in nucleus pulposus stromal cell somatic reprogramming and survival

Biomaterials delivery strategies to repair degenerated intervertebral discs by regulating the inflammatory microenvironment

Effect of potentiated cryopreserved mesenchymal stem cells in intervertebral disc repair

Contact Info

Product

Resources

About