Stem cells on regenerative and reproductive science in domestic animals

Pieri, Naira Caroline Godoy; Souza, Aline Fernanda de; Botigelli, Ramon César; Machado, Liliane S.; Ambrósio, Carlos Eduardo; Martins, Daniele dos Santos; Andrade, André Furugen Cesar de; Meirelles, Flávio Vieira; Hyttel, Poul; Bressan, Fabiana Fernandes

doi:10.1007/s11259-019-9744-6

Cited by 25 publications

(23 citation statements)

References 124 publications

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“…The generation of iPSCs from these species presents a major objective to facilitate the application of advanced reproductive technologies[41] including allowing easier genetic manipulation (knock-ins or knock-outs) in pluripotent cells used for chimera generation, improving SCNT efficiency by using iPSCs as nuclei donors[42,43] or producing functional gametes in vitro [44-46]. The first studies in cattle reported that the four Yamanaka factors were insufficient for inducing pluripotency, and that NANOG or NANOG plus LIN28 were additionally required[47,48].…”

Section: Domestic Ruminantsmentioning

confidence: 99%

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Induced pluripotent stem cells throughout the animal kingdom: Availability and applications

Pessôa

Bressan

Freude³

2019

WJSC

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Up until the mid 2000s, the capacity to generate every cell of an organism was exclusive to embryonic stem cells. In 2006, researchers Takahashi and Yamanaka developed an alternative method of generating embryonic-like stem cells from adult cells, which they coined induced pluripotent stem cells (iPSCs). Such iPSCs possess most of the advantages of embryonic stem cells without the ethical stigma associated with derivation of the latter. The possibility of generating “custom-made” pluripotent cells, ideal for patient-specific disease models, alongside their possible applications in regenerative medicine and reproduction, has drawn a lot of attention to the field with numbers of iPSC studies published growing exponentially. IPSCs have now been generated for a wide variety of species, including but not limited to, mouse, human, primate, wild felines, bovines, equines, birds and rodents, some of which still lack well-established embryonic stem cell lines. The paucity of robust characterization of some of these iPSC lines as well as the residual expression of transgenes involved in the reprogramming process still hampers the use of such cells in species preservation or medical research, underscoring the requirement for further investigations. Here, we provide an extensive overview of iPSC generated from a broad range of animal species including their potential applications and limitations.

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Section: Domestic Ruminantsmentioning

confidence: 99%

“…The swine, however, is considered one of the “non-permissive” species meaning that bona fide robust pluripotent stem cells derived from blastocysts-ESCs have not yet been successfully generated[41,76,77]. The generation of iPSCs is of great importance.…”

Section: Swinementioning

confidence: 99%

Induced pluripotent stem cells throughout the animal kingdom: Availability and applications

Pessôa

Bressan

Freude³

2019

WJSC

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“…iPSC technology suggests that although species-specific differences are evident, the basic mechanisms of pluripotency acquisition may follow similar patterns in mammals, and iPSCs have been reported from these animals [25][26][27]. Specifically, large farm animals are important models not only for pre-clinical stem cell therapies due to their physiological and morphological similarity to humans [28,29], but they also may greatly benefit from pluripotency in vitro to the generation of genetically superior or modified organisms for agricultural and biomedical applications, for example, for reproductive sciences [30].…”

Section: Introductionmentioning

confidence: 99%

“…A deeper understanding of the process of acquisition and maintenance of pluripotency and reprogramming in large domestic models will allow the optimization of several reproductive biotechnologies, the development of genetically engineered herds that may be useful as preclinical models for gene and cellular therapies, enhanced animal breeding programs and bioreactors [30]. This study tested whether the mechanisms used to induce pluripotency in human and mouse models can generate and maintain pluripotency in bovine and equine cells.…”

Section: Introductionmentioning

confidence: 99%

Generation of induced pluripotent stem cells from large domestic animals

et al. 2020

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Background: Induced pluripotent stem cells (iPSCs) have enormous potential in developmental biology studies and in cellular therapies. Although extensively studied and characterized in human and murine models, iPSCs from animals other than mice lack reproducible results. Methods: Herein, we describe the generation of robust iPSCs from equine and bovine cells through lentiviral transduction of murine or human transcription factors Oct4, Sox2, Klf4, and c-Myc and from human and murine cells using similar protocols, even when different supplementations were used. The iPSCs were analyzed regarding morphology, gene and protein expression of pluripotency factors, alkaline phosphatase detection, and spontaneous and induced differentiation. Results: Although embryonic-derived stem cells are yet not well characterized in domestic animals, generation of iPS cells from these species is possible through similar protocols used for mouse or human cells, enabling the use of pluripotent cells from large animals for basic or applied purposes. Herein, we also infer that bovine iPS (biPSCs) exhibit similarity to mouse iPSCs (miPSCs), whereas equine iPSs (eiPSCs) to human (hiPSCs). Conclusions: The generation of reproducible protocols in different animal species will provide an informative tool for producing in vitro autologous pluripotent cells from domestic animals. These cells will create new opportunities in animal breeding through transgenic technology and will support a new era of translational medicine with large animal models.

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“…In the context of this review, the term mesenchymal stromal cells has been adopted over mesenchymal stem cells due to the finite self-renewing property of MSCs that does not support the traditionally recognized self-renewing characteristic of stem cells [6]. The versatility of iPSCs may make them preferential over MSCs that are limited in their differentiation potential due to their multipotent nature [7][8][9]. ESCs offer a similar versatility to iPSCs as they are both pluripotent, but not without limitations [8].…”

Section: Introductionmentioning

confidence: 99%

The use of induced pluripotent stem cells in domestic animals: a narrative review

et al. 2020

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Induced pluripotent stem cells (iPSCs) are undifferentiated stem cells characterized by the ability to differentiate into any cell type in the body. iPSCs are a relatively new and rapidly developing technology in many fields of biology, including developmental anatomy and physiology, pathology, and toxicology. These cells have great potential in research as they are self-renewing and pluripotent with minimal ethical concerns. Protocols for their production have been developed for many domestic animal species, which have since been used to further our knowledge in the progression and treatment of diseases. This research is valuable both for veterinary medicine as well as for the prospect of translation to human medicine. Safety, cost, and feasibility are potential barriers for this technology that must be considered before widespread clinical adoption. This review will analyze the literature pertaining to iPSCs derived from various domestic species with a focus on iPSC production and characterization, applications for tissue and disease research, and applications for disease treatment.

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Stem cells on regenerative and reproductive science in domestic animals

Cited by 25 publications

References 124 publications

Induced pluripotent stem cells throughout the animal kingdom: Availability and applications

Induced pluripotent stem cells throughout the animal kingdom: Availability and applications

Generation of induced pluripotent stem cells from large domestic animals

The use of induced pluripotent stem cells in domestic animals: a narrative review

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