Stereology, morphometry, and mapping: the whole is greater than the sum of its parts

Glaser, Jack R.; Glaser, Edmund M.

doi:10.1016/s0891-0618(00)00073-9

Cited by 87 publications

(58 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Quantitative analyses of glandular inflammation were done with Stereoinvestigtor software v3.0 (MBF Bioscience, Williston, VT, USA) (Glaser and Glaser, 2000). Briefly, a contour was traced around each piece of gland, and a sampling grid of 400 µm x 400 µm was randomly placed over the contour.…”

Section: Antibody Analysismentioning

confidence: 99%

Type I Interferon Receptor Deficiency Prevents Murine Sjögren’s Syndrome

et al. 2013

View full text Add to dashboard Cite

In Sjögren's Syndrome (SS), inherent glandular defects, autoimmunity, and mononuclear cell infiltration within the salivary glands cause reduced salivation leading to xerostomia. Excessive production of type I interferons (IFN), triggered by environmental and genetic factors, is considered pathogenic in this disorder. However, whether type I IFN production is causative or an outcome of the disease process is not known. To address this question, we introduced a deficiency of interferon alpha receptor 1 (Ifnar1) into B6.Aec1Aec2 mice, which are known to have the genetic loci necessary for developing a SS-like disorder. This new mouse strain, B6.Aec1Aec2Ifnar1 -/-, lacking type I IFN-mediated signaling, was characterized for pilocarpine-induced salivation, the presence of serum autoantibodies, sialoadenitis, and dacryoadenitis. Compared with the B6.Aec1Aec2Ifnar1 +/+ (wild-type) mice, the B6.Aec1Aec2Ifnar1 -/-(knockout) mice had significantly lower mononuclear cell infiltration in the salivary and lacrimal glands. The knockout mice were completely protected from salivary gland dysfunction. Surprisingly, they had a robust autoantibody response comparable with that of the wild-type mice. These findings demonstrate that, in the absence of type I IFN-mediated signaling, systemic autoantibody responses can be dissociated from glandular pathology. Our study suggests that, in genetically susceptible individuals, the type I IFN pathway can instigate certain features of SS.

show abstract

Section: Antibody Analysismentioning

confidence: 99%

Type I Interferon Receptor Deficiency Prevents Murine Sjögren’s Syndrome

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Cells were counted only if they did not intersect forbidden lines. The optical fractionator method ( 25 ) was used to count TH-positive and TH-negative (cresyl violet-positive only) cellular profi les. Stereological counts were performed blindly by two independent investigators.…”

Section: Downloaded Frommentioning

confidence: 99%

Transgenic conversion of omega-6 into omega-3 fatty acids in a mouse model of Parkinson's disease

Bousquet

Gue

Émond

et al. 2011

Journal of Lipid Research

View full text Add to dashboard Cite

“…C.A.S.T. GRID, Olympus Denmark; StereoInvestigator, MicroBrightField, Inc.; Digital Stereology, Kinetic Imaging, Ltd.; Stereologer, Systems Planning and Analysis, Inc.; BIOQUANT Stereology Toolkit, R&M Biometrics, Inc.; for more information on contemporary stereological systems see Glaser and Glaser, 2000). Such systems can be too complex and expensive if number, numerical density or mean volume of particles, are of the main interest in the given biological experiment.…”

Section: Discussionmentioning

confidence: 99%

Disector Program for Unbiased Estimation of Particle Number, Numerical Density and Mean Volume

Tomori¹,

Krekule²,

Kubínová³

2011

Image Anal Stereol

View full text Add to dashboard Cite

A DISECTOR program is presented, offering the possibility to count particles by the disector or unbiased sampling brick principles as well as to apply the point-counting method needed for estimation of the particle volume density or mean particle volume. Three modes of counting, two semi-automatic and one automatic, are offered, allowing the user to choose the one most suitable for his image data. In a semi-automatic regime, the user marks and counts individual particles by a mouse during browsing through the stack of images. In the algorithm working in an automated mode, the role of a human operator is suppressed, assuming that segmented objects are available in individual levels. The settings of the point grid and 3-D probe can be tailored for each application. The DISECTOR program applications are shown on the examples of the estimation of the number and numerical density of mesophyll cells in a Norway spruce needle and the mean volume of tubular cells in a chick embryonic kidney.

show abstract

Stereology, morphometry, and mapping: the whole is greater than the sum of its parts

Cited by 87 publications

References 26 publications

Type I Interferon Receptor Deficiency Prevents Murine Sjögren’s Syndrome

Type I Interferon Receptor Deficiency Prevents Murine Sjögren’s Syndrome

Transgenic conversion of omega-6 into omega-3 fatty acids in a mouse model of Parkinson's disease

Disector Program for Unbiased Estimation of Particle Number, Numerical Density and Mean Volume

Contact Info

Product

Resources

About