Weight loss reality TV shows, which portray obese individuals and their struggle to lose weight, are highly popular. However, the shows often contain negative and stereotypical portrayals of obese individuals that can contribute to the formation of weight bias among viewers. In particular, adolescents might be highly susceptible to such portrayals, since physical appearance and body image play an important role during adolescence. In our experimental study, we investigated the priming effects of exposure to weight loss reality TV shows on implicit and explicit attitudes toward obese individuals among 353 adolescents. We exposed a treatment group (n = 173) to video clips of a weight loss reality TV show and a control group (n = 180) to video clips of an information-based TV magazine. Results indicated that for individuals who expressed a fear of being obese, exposure to the weight loss reality TV show reinforced negative explicit attitudes toward obese individuals by activating a perception of weight controllability. Exposure to the weight loss reality TV show also enhanced negative implicit attitudes toward obese individuals among all adolescents, irrespective of their fear of being obese. Altogether, our findings underscore the role of media in perpetuating weight bias. Western societies highly stigmatize obesity. Overweight or obese individuals of all ages face multiple forms of discrimination in different situations of daily life, including in health, employment, and educational settings. In perpetuating the stigmatization of obesity, or weight bias, media play an important role. On the one hand, results of content analytical research have provided evidence that various media genres demonstrate weight bias (Ata & Thompson, 2010). In particular, by emphasizing the lack of willpower, isolation, and laziness of obese individuals, reality TV shows depicting weight loss (e.g., The Biggest Loser, Secret Eaters, Extreme Weight Loss) promote the stigmatization and degradation of obese individuals (Sender & Sullivan, 2008). On the other hand, research on media priming has shown that stereotypical portrayals enhance stereotypical CONTACT Kathrin Karsay