Sterilization and preservation influence the biophysical properties of human amnion grafts

Versen-Hoeynck, F. von; Steinfeld, A.; Becker, Jakob; Hermel, Martin; Rath, Werner; Hesselbarth, Uwe

doi:10.1016/j.biologicals.2008.02.001

Cited by 51 publications

(40 citation statements)

References 49 publications

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“…Previous works have demonstrated that AD-AM possesses similar biophysical properties to AM preserved by storage in glycerol at 4°C and by deep freezing at 80% [19], but to our knowledge the present-study was the first attempt to compare the biological properties of PAA-sterilized AD-AM and Cryo-AM manufactured according to the procedure applied in this study. We focused on three clinically important aspects of cryo-preserved and PAA/ ethanol-sterilized air-dried AM: the release of wound-healing modulating soluble proteins, the preservation of the basement membrane and the ability to serve as a substrate for the cultivation of HLECs.…”

Section: Discussionmentioning

confidence: 87%

“…In AD-AM, the distribution of collagen IV and fibronectin was similar to that in Cryo-AM, but collagen VII laminin and laminin 5 were not traceable. Previous studies have also not detected collagen VII in AD-AM [19]. Since laminin 5 is connected to collagen VII [48], it is possible that the preservation process affects both proteins, leading to their destruction.…”

Section: Discussionmentioning

confidence: 89%

“…The AD-AM is produced according to a standardized procedure [19]. The process involves sterilization in a mixture containing peracetic acid (PAA) and ethanol for 4 h at room temperature.…”

Section: Discussionmentioning

confidence: 99%

“…The ready-to-use sterilized AD-AM (#GT1100, #GT1080) was obtained from the Deutsche Institut für Zell-und Gewebeersatz GmbH (DIZG Berlin, Germany). The preparation method of AD-AM used by DIZG has been described elsewhere [19].…”

Section: Preparation Of Ammentioning

confidence: 99%

“…Every step of the handling procedures, including preparation, preservation and sterilization, may affect the biological, chemical and physical properties of a biological material. Microscopic studies have revealed that different sterilization and preservation procedures influence the histological and biophysical properties of amniotic membrane [19,20]. Unfortunately, available data concerning the effect of each sterilization/preservation method on the biological function is rather limited.…”

Section: Introductionmentioning

confidence: 97%

See 4 more Smart Citations

Comparison of cryopreserved and air-dried human amniotic membrane for ophthalmologic applications

Thomasen

Pauklin

Steuhl

et al. 2009

Graefes Arch Clin Exp Ophthalmol

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show abstract

Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 89%

“…The AD-AM is produced according to a standardized procedure [19]. The process involves sterilization in a mixture containing peracetic acid (PAA) and ethanol for 4 h at room temperature.…”

Section: Discussionmentioning

confidence: 99%

Section: Preparation Of Ammentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

See 3 more Smart Citations

Comparison of cryopreserved and air-dried human amniotic membrane for ophthalmologic applications

Thomasen

Pauklin

Steuhl

et al. 2009

Graefes Arch Clin Exp Ophthalmol

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Amniotic membrane transplantation for acute ocular burns

Clare

Suleman

Bunce

et al. 2012

Cochrane Database of Systematic Reviews

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Assessment of fresh and preserved amniotic membrane for guided bone regeneration in mice

Fénelon

Etchebarne

Siadous

et al. 2020

J Biomedical Materials Res

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Thanks to its biological properties, the human amniotic membrane (HAM) can be used as a barrier membrane for guided bone regeneration (GBR). However, no study has assessed the influence of the preservation method of HAM for this application. This study aimed to establish the most suitable preservation method of HAM for GBR. Fresh (F), cryopreserved (C) lyophilized (L), and decellularized and lyophilized (DL) HAM were compared. The impact of preservation methods on collagen and glycosaminoglycans (GAG) content was evaluated using Masson's trichrome and alcian blue staining. Their suture retention strengths were assessed. In vitro, the osteogenic potential of human bone marrow mesenchymal stromal cells (hBMSCs) cultured on the four HAMs was evaluated using alkaline phosphatase staining and alizarin red quantification assay. In vivo, the effectiveness of fresh and preserved HAMs for GBR was assessed in a mice diaphyseal bone defect after 1 week or 1 month healing. Micro‐CT and histomorphometric analysis were performed. The major structural components of HAM (collagen and GAG) were preserved whatever the preservation method used. The tearing strength of DL‐HAM was significantly higher. In vitro, hBMSCs seeded on DL‐HAM displayed a stronger ALP staining, and alizarin red staining quantification was significantly higher at Day 14. In vivo, L‐HAM and DL‐HAM significantly enhanced early bone regeneration. One month after the surgery, only DL‐HAM slightly promoted bone regeneration. Several preserving methods of HAM have been studied for bone regeneration. Here, we have demonstrated that DL‐HAM achieved the most promising results for GBR.

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Sterilization and preservation influence the biophysical properties of human amnion grafts

Cited by 51 publications

References 49 publications

Comparison of cryopreserved and air-dried human amniotic membrane for ophthalmologic applications

Comparison of cryopreserved and air-dried human amniotic membrane for ophthalmologic applications

Amniotic membrane transplantation for acute ocular burns

Assessment of fresh and preserved amniotic membrane for guided bone regeneration in mice

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