Steroid and β-adrenergic receptor modifications in target organs of broiler chickens fed with a diet containing β2-adrenergic agents

Badino, P.; Odore, R.; Pagliasso, S.; Schiavone, Achille; Girardi, C.

doi:10.1016/j.fct.2008.02.025

Cited by 10 publications

(6 citation statements)

References 62 publications

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“…The data obtained in the subacute experiment are in line with former reports that demonstrated a downregulation of β 2 ‐adrenergic receptors in various experimental animals after a prolonged CLB treatment (Schiavone et al. , 2004; Badino et al. , 2008).…”

Section: Discussionsupporting

confidence: 91%

Effects of the β2-agonist clenbuterol on testicular steroidogenic acute regulatory protein mRNA expression in adult rats

Zhu

2010

Journal of Veterinary Pharmacology and Therapeutics

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This study was carried out to investigate the effects of clenbuterol (CLB) on the testicular (steroidogenic acute regulatory, StAR) protein mRNA expression in rats. Thirty adult male rats were administered CLB by gavage daily at the doses of 0.4, 2.0 and 18.5 mg/kg bw for 14 days in the subacute experiment, whereas 20 rats received a single treatment with CLB at the doses of 20 and 40 mg/kg bw in the acute experiment and 20 rats were treated with 0.9% NaCl solution as vehicle groups. Testicular tissues were collected and snap-frozen in liquid nitrogen and stored at -70 °C until use. The levels of StAR mRNA were detected by RT-PCR. The levels of StAR mRNA were markedly increased (P < 0.05) at both dosages of 20 and 40 mg/kg bw but the effects were not dose-dependent and the mRNA levels of StAR were returned to near normal level after 7 days of CLB withdrawal, compared with the control animals. In the subacute experiment, CLB induced a dose-dependent but no statistical significant reduction (P > 0.05) in the expression levels of StAR mRNA, and the mRNA levels were recovered to near normal level in the groups treated with CLB at dosages of 0.4 and 2.0 mg/kg bw/day following a 7-day withdrawal period, compared with the control animals. The mRNA levels of StAR showed a significant decrease in the groups treated with CLB at the dosage of 18.5 mg/kg bw/day (P < 0.05) after a 1- or 7-day withdrawal period with respect to the control animals. These results demonstrated transient stimulative effects of CLB on testicular StAR mRNA levels and inhibitory effects after treatment with CLB for 14 consecutive days.

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Section: Discussionsupporting

confidence: 91%

Effects of the β2-agonist clenbuterol on testicular steroidogenic acute regulatory protein mRNA expression in adult rats

Zhu

2010

Journal of Veterinary Pharmacology and Therapeutics

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“…Clenbuterol, a typical weight loss agent, is a kind of β2-adrenergic agonist, and was illegally used as a feed additive to improve production performance and a carcass composition in many countries [3]–[4]. With the increased use of internet sales, the internet has made this even banned product to be readily accessible for the aim of weight loss or dieting addition [2].…”

Section: Introductionmentioning

confidence: 99%

“…Administration of growth-promoting doses of clenbuterol adversely affected the liver function in female pigs [10]. Dietary administration of clenbuterol decreased androgen receptor (AnR) expression in testicle, glucocorticoid receptor (GR) expression in lymphoid tissues, and β-adrenergic receptor (β-AR) expression in targeted organs of chickens [3]. Clenbuterol caused an impairment of collagen turnover by down-regulating MMP-9 activity [11].…”

Section: Introductionmentioning

confidence: 99%

Adverse Effects from Clenbuterol and Ractopamine on Nematode Caenorhabditis elegans and the Underlying Mechanism

Zhuang

Zhao

et al. 2014

PLoS ONE

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In the present study, we used Caenorhabditis elegans assay system to investigate in vivo toxicity from clentuberol and ractopamine and the possible underlying mechanism. Both acute and prolonged exposures to clentuberol or ractopamine decreased brood size and locomotion behavior, and induced intestinal autofluorescence and reactive oxygen species (ROS) production. Although acute exposure to the examined concentrations of clentuberol or ractopamine did not induce lethality, prolonged exposure to 10 µg/L of clentuberol and ractopamine reduced lifespan. At relatively high concentrations, ractopamine exhibited more severe toxicity than clentuberol on nematodes. Overexpression of sod-2 gene encoding a Mn-SOD to prevent induction of oxidative stress effectively inhibited toxicity from clentuberol or ractopamine. Besides oxidative stress, we found that clentuberol might reduce lifespan through influencing insulin/IGF signaling pathway; however, ractopamine might reduce lifespan through affecting both insulin/IGF signaling pathway and TOR signaling pathway. Ractopamine more severely decreased expression levels of daf-16, sgk-1, skn-1, and aak-2 genes than clentuberol, and increased expression levels of daf-2 and age-1 genes at the examined concentration. Therefore, the C. elegans assay system may be useful for assessing the possible toxicity from weight loss agents, and clentuberol and ractopamine may induce toxicity through different molecular mechanisms.

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“…It found that clenbuterol at concentrations 10 −7 M and 10 −8 M stimulated the proliferation of a hormone-dependent human breast cancer line (CG-5) in vitro. Furthermore, results of in vivo studies on chicken suggest the role of clenbuterol as a growth stimulator (Badino et al, 2008). Claudine et al (1999) found that clenbuterol as a β2-adrenergic receptor-selective agonist prevented Jo2-mediated liver apoptosis and death in F28 mice.…”

Section: Resultsmentioning

confidence: 99%

Mutagenicity and DNA-damaging potential of clenbuterol and its metabolite 4-amino-3,5-dichlorobenzoic acid in vitro

Vulić

Durgo

Pleadin

et al. 2015

Food and Chemical Toxicology

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Steroid and β-adrenergic receptor modifications in target organs of broiler chickens fed with a diet containing β2-adrenergic agents

Cited by 10 publications

References 62 publications

Effects of the β2-agonist clenbuterol on testicular steroidogenic acute regulatory protein mRNA expression in adult rats

Effects of the β2-agonist clenbuterol on testicular steroidogenic acute regulatory protein mRNA expression in adult rats

Adverse Effects from Clenbuterol and Ractopamine on Nematode Caenorhabditis elegans and the Underlying Mechanism

Mutagenicity and DNA-damaging potential of clenbuterol and its metabolite 4-amino-3,5-dichlorobenzoic acid in vitro

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