Steroidal saponin profiles and their key genes for synthesis and regulation in Asparagus officinalis L. by joint analysis of metabolomics and transcriptomics

Cheng, Qiqun; Zeng, Liangqin; Wen, Hao; Brown, Sylvia E; Li, Xingyu; Lin, Chun Yi; Liu, Zhengjie; Mao, Zichao

doi:10.1186/s12870-023-04222-x

Cited by 10 publications

(2 citation statements)

References 37 publications

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“…Additionally, various TFs have been shown to play crucial roles in plant growth, development, metabolic pathways, and stress responses. For instance, ZFs, MYBs, and WRKYs [ 47 ] are predicted to be associated with the synthesis of steroid saponin in Asparagus officinalis L. Similarly, WRKYs, MYBs, and bHLHs [ 48 ] are speculated to be associated with steroid and terpene skeleton biosynthesis in Trillium govanianum . Several studies have shown the involvement of WRKY, AP2/ERF, and other TFs [ 49 , 50 ] in growth and developmental processes mediated by BRs.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide methylation, transcriptome and characteristic metabolites reveal the balance between diosgenin and brassinosteroids in Dioscorea zingiberensis

Li,

Geng

et al. 2024

Horticulture Research

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Diosgenin (DG) is a bioactive metabolite isolated from Dioscorea species, renowned for its medicinal properties. Brassinosteroids (BRs) are a class of crucial plant steroidal hormones. Cholesterol and campesterol are important intermediates of DG and BRs biosynthesis, respectively. DG and BRs are structurally similar components, however, the regulatory network and metabolic interplays have not been fully elucidated. In an effort to decode these complex networks, we conducted a comprehensive study integrating genome-wide methylation, transcriptome and characteristic metabolites data from Dioscorea zingiberensis. Leveraging these data, we were able to construct a comprehensive regulatory network linking DG and BRs. Mass spectrometry results enabled us to clarify the alterations in cholesterol, campesterol, diosgenin and castasterone (one of the major active BRs). The DG content decreased by 27.72% at 6 h following brassinolide treatment, whereas the content increased by 85.34% at 6 h after brassinazole treatment. Moreover, we pinpointed DG/BR-related genes, such as CASs, CYP90s and B3-ARFs, implicated in the metabolic pathways of DG and BRs. Moreover, CASs and CYP90s exhibit hypomethylation, which is closely related to their high transcription. These findings provide robust evidence for the homeostasis between DG and BRs. In conclusion, our research revealed the existence of a balance between DG and BRs in D. zingiberensis. Furthermore, our work not only provides new insights into the relationship between the two pathways but also offers a fresh perspective on the functions of secondary metabolites.

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Section: Discussionmentioning

confidence: 99%

Genome-wide methylation, transcriptome and characteristic metabolites reveal the balance between diosgenin and brassinosteroids in Dioscorea zingiberensis

Li,

Geng

et al. 2024

Horticulture Research

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“…At present, metabolites can be analyzed by gas chromatography-mass spectrometry (GC-MS) or LC-MS to study gene-induced metabolic disorders [42,43]. In clinical practice, the targeting analysis of certain known compounds is usually used to determine the occurrence and development of the disease.…”

Section: Targeted and Untargeted Analysismentioning

confidence: 99%

Review on analytical technologies and applications in metabolomics

MENG,

LIU,

et al. 2024

BIOCELL

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Over the past decade, the swift advancement of metabolomics can be credited to significant progress in technologies such as mass spectrometry, nuclear magnetic resonance, and multivariate statistics. Currently, metabolomics garners widespread application across diverse fields including drug research and development, early disease detection, toxicology, food and nutrition science, biology, prescription, and chinmedomics, among others.Metabolomics serves as an effective characterization technique, offering insights into physiological process alterations in vivo. These changes may result from various exogenous factors like environmental conditions, stress, medications, as well as endogenous elements including genetic and protein-based influences. The potential scientific outcomes gleaned from these insights have catalyzed the formulation of innovative methods, poised to further broaden the scope of this domain. Today, metabolomics has evolved into a valuable and widely accepted instrument in the life sciences. However, comprehensive reviews focusing on the sample preparation and analytical methodologies employed in metabolomics within the life sciences are surprisingly scant. This review aims to fill that gap, providing an overview of current trends and recent advancements in metabolomics. Particular emphasis is placed on sample preparation, sophisticated analytical techniques, and their applications in life science research.

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Two previously undescribed cholestanol saponins from the rhizomes of Paris fargesii var. petiolata

Tian,

Bi,

Chen

et al. 2024

Fitoterapia

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Steroidal saponin profiles and their key genes for synthesis and regulation in Asparagus officinalis L. by joint analysis of metabolomics and transcriptomics

Cited by 10 publications

References 37 publications

Genome-wide methylation, transcriptome and characteristic metabolites reveal the balance between diosgenin and brassinosteroids in Dioscorea zingiberensis

Genome-wide methylation, transcriptome and characteristic metabolites reveal the balance between diosgenin and brassinosteroids in Dioscorea zingiberensis

Review on analytical technologies and applications in metabolomics

Two previously undescribed cholestanol saponins from the rhizomes of Paris fargesii var. petiolata

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