Stigmasterol and Stigmasterone from Methanol Extract of Calophyllum soulattri Burm. F. Stem Bark

Marliyana, Soerya Dewi; Wibowo, Fajar Rakhman; Handayani, Desi Suci; Kusumaningsih, Triana; Suryanti, Venty; Firdaus, Maulidan; Annisa, Ema Nur

doi:10.14710/jksa.24.4.108-113

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…Based on FTIR results and compared with literature data, the isolated compound is stigmasterol [12].…”

Section: Fig2 Ft-ir Chromatogrammentioning

confidence: 99%

Isolation of Antioxidant and Antibacterial Compound from Solanum ferox L Leaves

Hazimah,

Haiyul,

Dina

et al. 2023

BIO Web Conf.

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The sour eggplant plant (Solanum ferox L), a vegetable that is frequently used in cooking and is a member of the Solanaceae family, is also used as an antipyretic and to treat syphilis, body aches, lack of appetite, fever, itching, cuts, and bruising. This is due to the presence of several bioactive chemicals that have toxic, rheumatoid, antiviral, anticancer, antibacterial, and anti-asthma properties. The objective of this work was to separate antioxidant and antibacterial substances from Solanum ferox L. leaves. N-hexane and methanol were used to extract the leaves of Solanum ferox. The characterization of chemicals in the n-hexane extract was done while the antioxidant and antibacterial properties of both extracts were examined. UV-Vis spectra, FTIR, proton and carbon nuclear magnetic resonance (1H-NMR and 13C-NMR), and FTIR were used to characterize the compound). Using vitamin C as a reference and the DPPH (1,1-diphenyl-2-picrylhydrazyl) technique to measure free radicals, the antioxidant activity of the Solanum ferox plant extract was assessed. E. coli, S. aureus, and S. subtilis bacteria are used in an agar diffusion method test for antimicrobial activity. The solvent used to dissolve the sample served as the negative control while amoxsan served as the positive standard. One pure substance that has been isolated with success is stigmasterol, with the chemical formula C29H48O. The IC50 of Solanum ferox leaf n-hexane extract at 1218.5 ppm, which included not active, indicated that the IC50 of Solanum ferox leaf methanol extract at 268.5 ppm was ineffective. E. coli is inhibited by n-hexane extract in antibacterial tests at an inhibition value of 9.27 mm. Methanol extracts had an inhibitory potency of 7.82 mm against E. coli and 11.29 mm against S. aureus.

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“…Based on FTIR results and compared with literature data, the isolated compound is stigmasterol [12].…”

Section: Fig2 Ft-ir Chromatogrammentioning

confidence: 99%

Isolation of Antioxidant and Antibacterial Compound from Solanum ferox L Leaves

Hazimah,

Haiyul,

Dina

et al. 2023

BIO Web Conf.

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“…C-H stretch, sharp[22],[26] 3293.1 ---3400-3200 -OH stretching, broad [22], [26]  ISSN: 2252-8814 Int J Adv Appl Sci, Vol. 12, No.…”

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confidence: 99%

Double step method in lipid extraction from biomass Aurantiochytrium sp powder

Sulistiawati,

Suhendra,

Setyawan

et al. 2023

IJAAS

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Aurantiochytrium sp is a marine microalgae that is rich in lipids. Extraction of lipids from microalgae requires effort to select a suitable solvent and extraction method. This research used a double-step extraction method to study a mixture of n-hexane and methanol as a solvent. The variables studied were stirring time, the n-hexane to methanol (H/M) mixture ratio, and the solvent-to-biomass ratio (S/B). This research concluded that an optimum stirring time was 30 min, and a mixture of n-hexane and methanol solvents with a volume ratio of 1:1 is optimum. The optimum solvent-to-biomass ratio was S/B=20 mL/g dry microalgae. Under these conditions, the yield of oil was 83.88%. Double-step extraction can increase the yield by 10-40%.

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