STIM1-ORAI1 direct interaction cannot govern store-operated calcium entry (SOCE) in platelets

Abstract

Store-operated calcium entry (SOCE) plays an important role in platelet function. It is generally assumed that the mechanism of SOCE relies on the direct interaction of STIM1 and ORAI1 proteins with specific STIM1:ORAI1 stoichiometry. However, in platelets, other pathways may take place. Here we aim to investigate the mechanisms of SOCE in platelets. We developed a lattice-based mathematical model that represented STIM1-ORAI1 interactions and applied it to both HEK cells, where SOCE mechanism is well established, and platelets. The model was able to describe STIM1-ORAI1 behavior in HEK cells successfully. We used the same parameters for protein interaction and applied them to platelets. As a result, we demonstrated that the number of STIM1 proteins on ER membrane could not assure the needed stoichiometry to proper SOCE in platelets.

Systems Biology and Physiology Reports in 2021: a yearly report

“…After a year of development, SBPReports boasts a highly professional and dedicated editorial board formed by experts in the systems biology field from Belarus, Canada, China, India, Russia, UK and USA. The three major sections of the edition are related to the three major levels of understanding of the biological systems: molecular [1,2], cellular [3,4], and organism [5,6] levels. The papers published in the four issues during this year include original articles [1,2,4,6], reviews [5,[7][8][9], and methodological studies [10,11].…”

“…The three major sections of the edition are related to the three major levels of understanding of the biological systems: molecular [1,2], cellular [3,4], and organism [5,6] levels. The papers published in the four issues during this year include original articles [1,2,4,6], reviews [5,[7][8][9], and methodological studies [10,11]. In addition, two large abstract collections Systems Biology and Systems Physiology Congress 2021 and International Congress devoted to the Memory of the Founders of Biophysics Department of Physics Faculty of Lomonosov Moscow State Universityprofessor L.A. Blumenfeld and professor S.E.…”