Stimulated Raman scattering microscopy: an emerging tool for drug discovery

Tipping, William J.; Lee, Martin; Serrels, Alan; Brunton, Valerie G.; Hulme, Alison N.

doi:10.1039/c5cs00693g

Cited by 216 publications

(167 citation statements)

References 111 publications

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“…In an elegantly performed study, the authors show that ovarian cancer stem cells and cancer cells grown as spheroids have a higher ratio of unsaturated to saturated fatty acids, and that this ratio is essential for the cells to retain stemness. To perform the study, Li et al use single cell stimulated Raman scattering (SRS) microscopy to compare the lipid content and the levels of unsaturation in the intracellular lipid pools of living single cells (Tipping et al, 2016). By scanning single cells with a femtosecond laser and measuring Raman shifts pertaining to lipid C-H vibrations, the authors show that ovarian CSCs have significantly higher levels of lipid droplets, and that these droplets contain a higher percentage of unsaturated lipids.…”

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confidence: 99%

Unsaturated Fatty Acids Maintain Cancer Cell Stemness

2017

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confidence: 99%

Unsaturated Fatty Acids Maintain Cancer Cell Stemness

2017

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“…As a result of the added benefit of lack of non-resonant background in SRS compared to CARS, as well as a linear concentration dependence and lack of spectral distortion, SRS could perhaps provide the answer to high throughput spectroscopic drug screening. Tipping et al [20] recently reviewed SRS as "an emerging tool for drug discovery". In this review, the potential for SRS as the latest development for in vitro drug screening is explored, pointing towards SRS as the future for spectroscopic HTS.…”

Section: An Alternative Technique -Surface Enhanced Raman Spectroscopmentioning

confidence: 99%

“…They are nonlinear Raman techniques, which overcome the inherent weakness of spontaneous Raman spectroscopy. [20] Therefore, measurements can be performed faster, a vital benefit for HTS. This review will therefore focus on the potential of vibrational spectroscopy for use in analysing the interaction of drugs with cells and, in particular, how these techniques could potentially be employed for the screening of drug candidates in a high throughput manner, a label free method providing a wealth of biochemical information that could reveal important information regarding the interaction of drug candidates with cells.…”

Section: Introductionmentioning

confidence: 99%

Vibrational spectroscopy as a tool for studying drug-cell interaction: Could high throughput vibrational spectroscopic screening improve drug development?

Jamieson

Byrne²

2017

Vibrational Spectroscopy

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. (2017). Vibrational spectroscopy as a tool for studying drug-cell interaction: could high throughput vibrational spectroscopic screening improve drug development? Vibrational Spectroscopy, vol. 91, July, pp. 16-30. doi.org/10.1016/ j.vibspec.2016 Vibrational spectroscopy as a tool for studying drug-cell interaction: could high throughput vibrational spectroscopic screening improve drug development? AbstractVibrational spectroscopy is currently widely explored as a tool in biomedical applications. An area at the forefront of this field is the use of vibrational spectroscopy for disease diagnosis, ultimately aiming towards spectral pathology. However, while this field shows promising results, moving this technique into the clinic faces the challenges of widespread clinical trials and legislative approval. While spectral pathology has received a lot of attention, there are many other biomedical applications of vibrational spectroscopy, which could potentially be translated to applications with greater ease. A particularly promising application is the use of vibrational spectroscopic techniques to study the interaction of drugs with cells. Many studies have demonstrated the ability to detect biochemical changes in cells in response to drug application, using both infrared and Raman spectroscopy. This has shown potential for use in high throughput screening (HTS) applications, for screening of efficacy and mode of action of potential drug candidates, to speed up the drug discovery process. HTS is still a relatively new and growing area of research and, therefore, there is more potential for new techniques to move into and shape this field. Vibrational spectroscopic techniques come with many benefits over the techniques used currently in HTS, primarily based on fluorescence assays to detect specific binding interactions or phenotypes. They are label free, and an infrared or Raman spectrum provides a wealth of biochemical information, and therefore could reveal not only information about a specific interaction, but about how the overall biochemistry of a cell changes in response to application of a drug candidate. Therefore, drug mode of action could be elucidated. This review will investigate the potential for vibrational spectroscopy, particularly FTIR and Raman spectroscopy, to benefit the field of HTS and improve the drug development process. In addition to FTIR and Raman spectroscopy, surface enhanced Raman spectroscopy (SERS), coherent anti-Stokes Raman spectroscopy (CARS) and stimulated Raman spectroscopy (SRS), will be investigated as an alternative tool in the HTS process.

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“…Im Gegensatz dazu ist die konfokale Raman-Mikroskopie eine markerfreie Imaging-Methode,d ie fähig ist, kleine Moleküle in Zellen zu lokalisieren. [11][12][13] Die Raman-Empfindlichkeit ist jedoch begrenzt, und in früheren Studien wurden hçhere Konzentrationen (2 mm-100 mm)v on Molekülen verwendet, um geeignete Signal-Rausch-Verhältnisse zu erzielen. [11][12][13][15][16][17] Hier verfolgen wir die Aufnahme,d ie intrazelluläre räumliche Verteilung und den Metabolismus von Neratinib in signifikant niedrigeren Konzentrationen (0.5-10 mm)i nv erschiedenen Krebszellen unter Verwendung markerfreier Raman-Mikrospektroskopie,w omit eine Annäherung an die klinisch relevanten Konzentrationen erzielt wurde.H ER2-positive Brustkrebszellen (SK-BR-3) und NSCLC-Zellen mit ,d as Neratinib repräsentiert.…”

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